...Tom P. Fleming; Mark Hay; Qamar Javed; Sandra Citi ABSTRACT The molecular maturation of the tight junction in the mouse early embryo has been investigated by monitoring the distribution of cingulin, a 140 10 3 M r peripheral (cytoplasmic) membrane constituent of the junction, at different stages...

...Qamar Javed; Tom P. Fleming; Mark Hay; Sandra Citi ABSTRACT The expression of the tight junction peripheral membrane protein, cingulin (140 10 3 M r ), was investigated in mouse eggs and staged preimplantation embryos by immunoblotting and immunoprecipitation. Polyclonal antibody to chicken brush...

... this period, initial focal junction sites gradually extend laterally to become zonular and acquire the peripheral tight junction proteins ZO-1 and cingulin. Our studies indicate that junction components assemble in a temporal sequence with ZO-1 assembly preceding that of cingulin, suggesting that the junction...