... Regulation Chimera Considerable research activity is currently focused on a variety of human organoids and similar self-organizing structures such as gastruloids (hereafter collectively referred to as ʻorganoids', unless otherwise specified). Such research has already led to an enhanced...

...). With rodent PSCs, chimera formation using pre-implantation embryos is the gold-standard assay of pluripotency (competence of progeny to differentiate into all three germ layers). In human PSCs (hPSCs), however, this can only be monitored via teratoma formation or in vitro differentiation, as ethical concerns...

... and its receptor in the embryonic gut, but not in the BF, further supports an ectodermal origin for the bursal rudiment. Using chick-quail chimeras, quail tail bud ectoderm was homotopically transplanted into ectoderm-ablated chick, resulting in quail-derived bursal follicle formation. Chimeric bursal...

... ( Schoonjans et al., 2003 ), and genotyped by PCR and Southern blotting( Chang et al., 1999 ). Chimeras between ES cell lines and diploid Rosa-βgeo-26 morulae were generated as described ( Goumans et al.,1999 ). The majority of wild-type→WT lacZ ,Smad5 +/m1 →WT lacZ , and of Smad5 m1/m1 →WT lacZ...

... of a subset of posterior mesendodermal genes, and loss of ability to induce the DVE. The loss of some DVE genes such as Hex and goosecoid is rescued in chimeras where only the epiblast was wild type; however, these DVE markers were no longer restricted distally but covered the entire epiblast. Thus, the Apc...

... expression in the Müllerian duct is dynamic, corresponding to its formation and differentiation in females and regression in males. Although female Lim1 -null neonates had ovaries they lacked a uterus and oviducts. A novel female mouse chimera assay was developed and revealed that Lim1 is required cell...

... Schwann cells in peripheral cranial nerves, pericytes in the choroid membrane and around the endothelial wall of the blood vessels, as well as loose mesenchyme in the head ( Fig. 7 B,D). Their participation in cartilage was investigated in E8 chimeras ( n =3): in the diencephalic region, the sella turcica...

...-mail: [email protected] and [email protected] ) 8 3 2002 © 2002. 2002 Stem cells Endothelium Pericyte Angiogenesis Chimera Mesoderm Histogenesis Mouse Quail Our current appreciation of stem cells plasticity has changed dramatically in the last years, following the report...

... for correspondence (e-mail [email protected] ) 15 12 2000 08 02 2000 © 2000 by Company of Biologists 2000 Pax6 Mouse chimaera Chimera Eye morphogenesis Correctly regulated expression of wild-type Pax6 is necessary for normal development of the mammalian CNS and sensory organs...

... was composed of predominantly wild-type cells, we found that Lim1 −/− cells were able to contribute to the anterior mesendoderm of embryonic day 7.5 chimeric embryos but that embryonic day 9.5 chimeric embryos displayed a range of head defects. In addition, early somite stage chimeras generated by injecting...

... cells when analysed in the adult doubly homozygous mutant chimeras. These examples strongly support a primary and cell autonomous role for TEK in maturing endothelia during embryonic development. In conclusion, our studies have demonstrated that TEK and TIE do not mediate a redundant function...

... for correspondence (e-mail: [email protected]; [email protected] ) 25 05 1999 19 07 1999 © 1999 by Company of Biologists 1999 Pericyte Telencephalon Vascularization Neural crest Meninges Forebrain Chimera Chick Quail After neurulation, the cells of the vertebrate...

[email protected] ) 27 10 1999 27 11 1999 © 1999 by Company of Biologists 1999 Regeneration Blastema formation Dorsoventral interaction Planarian Chimera Transplantation The planarian is remarkable in its ability to regenerate. Even a small fragment cut from...

...Neil A. McHale; Michael Marcotrigiano ABSTRACT The role of LAM1 in dorsoventrality and lateral growth of the leaf blade was investigated in the ‘bladeless’ lam1 mutant of Nicotiana sylvestris and in periclinal chimeras with lam1 and wild-type ( N. glauca ) cell layers. Mutant lam1 primordia show...

... ectoderm. * Author for correspondence (e-mail: [email protected] ) 10 06 1998 06 08 1998 © 1998 by Company of Biologists 1998 Sex-chromosome aneuploidy X chromosome inactivation Genomic imprinting Mouse lacZ transgene Chimera Studies mainly in humans...

..., the intercostal muscles and the distal ribs were deficient, while the proximal ribs were more or less normal. Quail tissues including the dermomyotome, the ectoderm and the medial edge of lateral plate, were transplanted to replace chick dermomyotomes. In these chimeras, the ribs, which would be deficient without...

... to an apparently identical phenotype. In this study, we demonstrate by chimera analysis that the cardiomyocyte phenotype in RXR α −/− embryos is a non-cell-autonomous phenotype. In chimeric embryos made with embryonic stem cells lacking RXRα, cardiomyocytes deficient in RXRα develop normally and contribute...

.... (1995) , except that the pair of primers for detection of the targeted allele were: forward 5′-aacaccgagcgaccctgcag-3′ and reverse 5′-agagcagccgattgtctgttgt-3′. Staining of whole mounts to visualize lacZ expression in chimeras was performed as described by Hogan et al. (1994) . For staining...

..., and subsequently in the neuroectoderm for the specification of forebrain and midbrain territories. * Author for correspondence (e-mail: [email protected] ) 12 12 1998 04 02 1998 © 1998 by Company of Biologists 1998 Otx2 forebrain midbrain patterning chimera ES cell...

...K. John McLaughlin; Helga Kochanowski; Davor Solter; Georg Schwarzkopf; Piroska E. Szabó; Jeffrey R. Mann ABSTRACT Mouse chimeras made with androgenetic (two paternal genomes) ova or embryonic stem cells frequently die at the perinatal stage and exhibit a range of defects, the most noticeable being...