... RNAi Primary cilium Midline crossing Ift88 Arl13b Mouse Chicken Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung http://dx.doi.org/10.13039/501100001711 Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659 SFB 488...

...-137 Chicken Agencia Nacional de Promoción Científica y Tecnológica http://dx.doi.org/10.13039/501100003074 PICT 2018-1879 In all chordates, the ectoderm layer becomes segregated into the neural plate (NP) and the surrounding non-neural ectoderm. The NP subsequently folds...

... at tyrosine 489 by Abelson kinase, to the distal axon, which in turn is necessary for the correct navigation of post-crossing commissural axons in the developing chicken spinal cord. * Author for correspondence ( [email protected] ) Handling Editor: James Briscoe Competing interests...

...Martin A. Estermann; Andrew T. Major; Craig A. Smith ABSTRACT During gonadal sex determination, the supporting cell lineage differentiates into Sertoli cells in males and pre-granulosa cells in females. Recently, single cell RNA-seq data have indicated that chicken steroidogenic cells are derived...

... are poorly understood. In this study, we use single-cell transcriptomics to define the cell-fate decisions directing LPM specification, subdivision and early initiation of the forelimb mesenchyme in chicken embryos. We establish a transcriptional atlas and global cell-cell signalling interactions...

... by The Company of Biologists Ltd 2022 Summary: This Review presents the intrinsic and extrinsic mechanical forces acting on the embryonic gut and their determining role in structuring the development of this organ. Biomechanics Intestine Embryo Mouse Chicken Human Université de Paris...

... live imaging of anterior epiblast cells at the brain-forming stages in avian embryos is reported, revealing their long-distance migration and interaction with the anterior mesendoderm to form brain tissues. Epiblast Node Anterior mesendoderm Brain primordia Live imaging Chicken...

... physiological (molting) and plucking injury-induced regeneration in chickens. Proliferation analyses reveal quiescent, transient-amplifying (TA) and long-term label-retaining dermal cell (LRDC) states. During the growth phase, LRDCs are activated to make new dermal components with distinct cellular flows...

... ). A series of normal stages in the development of the chick embryo . J. Morphol. 88 , 49 - 92 . 10.1002/jmor.1050880104 Harris , M. P. , Hasso , S. M. , Ferguson , M. W. J. and Fallon , J. F. ( 2006 ). The development of archosaurian first-generation teeth in a chicken mutant...

... to systematically annotate the functions of lncRNAs in the chicken genome. To monitor gene expression changes during feather regeneration, we took an unbiased whole-genome profiling approach based on next-generation sequencing technology (RNA-seq data deposited in GEO under the accession number GSE42017 ; Chu...

... kinase (LTK), an ALK-type receptor tyrosine kinase, is expressed by neural crest cells during early migratory stages in chicken embryos. Loss of LTK in the cranial neural crest impairs migration and results in increased levels of apoptosis. Conversely, midkine, previously proposed as a ligand for ALK...

... unclear how the keratinocytes sense this gradient and orient barb growth. Here, we show that in chicken, owing to feather branching, the global Wnt gradient is subdivided into periodic barbs. Within each barb, the anterior barbule plate cells tilt before the posterior cells. The core planar cell polarity...

... is a large HSPG that is abundantly expressed in the developing brain and in virtually all basal laminae of the developing organs. Agrin is also expressed by Schwann cell precursors, immature Schwann cells ( Lasrado et al., 2017 ) and developing chicken sensory ganglia ( Halfter et al., 1997 ). It has been...

... an optimised, adaptable toolkit enabling genome and epigenome engineering in the chicken embryo, and demonstrate its utility by probing gene regulatory interactions mediated by neural crest enhancers. First, we optimise novel efficient guide-RNA mini expression vectors utilising chick U6 promoters, provide...

..., which is upregulated in mutant lateral LMC motor neurons. Genetic and molecular analyses showed that the mutation acts in the EphA4–Limk1–Cfl1/cofilin–actin pathway to modulate growth cone extension/collapse. In the chicken, both experimental upregulation of Limk1 by electroporation and pharmacological...

... construct, which serves as a dominant-negative form by repressing transcription of GATA3 downstream genes. Such a strategy has previously been used for Gata4 ( Kamei et al., 2011 ). Infection with m Gata3 -EnR in chicken limbs resulted in a spectrum of malformations, with some of the characteristics similar...

...Laurent Yvernogeau; Catherine Robin Hematopoietic stem cells (HSCs), which are responsible for blood cell production, are generated during embryonic development. Human and chicken embryos share features that position the chicken as a reliable and accessible alternative model to study developmental...

...Lorna Taylor; Daniel F. Carlson; Sunil Nandi; Adrian Sherman; Scott C. Fahrenkrug; Michael J. McGrew In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 ( vasa ) locus in chicken primordial germ cells (PGCs). Vasa is a key germ cell determinant in many animal...

... , transcription factors essential for vestibular development, through the canonical pSMAD pathway and a non-canonical pathway, respectively. Dorsalization Inner ear Otocyst Polarization Signaling Chicken The inner ear is a morphologically complex three-dimensional organ with regionally...

... by rescue experiments ( Fig. 5 ). The effect on midline crossing induced by a miR targeting chicken RabGDI was rescued by co-electroporation of a plasmid encoding human RABGDI that was not targeted by miRabGDI ( Fig. 5 A). The effect of loss of calsyntenin 1, induced by dsRNA derived from the 3′ UTR...