... to radially sort and myelinate peripheral axons. Elevation of cAMP, but not Rac1 activity, in csp −/− restores myelin ensheathment. Most importantly, we show a significant decrease in laminin expression within csp −/− posterior lateral line nerve and that forcing Laminin 2 expression in csp −/− fully restores...

... of the signalling pathways underlying the balance of GluR subtypes, we performed an RNA interference screen of candidate genes and found that postsynaptic-specific knockdown of dunce , which encodes cAMP phosphodiesterase, increased levels of GluR subtype A but decreased subtype B. Furthermore, bidirectional...

...Nancy Nader; Raphael Courjaret; Maya Dib; Rashmi P. Kulkarni; Khaled Machaca Vertebrate oocytes arrest at prophase of meiosis I as a result of high levels of cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) activity. In Xenopus , progesterone is believed to release meiotic arrest...

...% develop a prolapse of the hindgut by the late prism stage (∼8 h after C5a protein expression normally peaks). Since C5a orthologs efflux cyclic nucleotides, and cAMP-dependent protein kinase ( Sp -CAPK/PKA) is expressed in pigment cells, we examined whether C5a could be involved in gastrulation through...

... stage of meiotic prophase I. In the current study, we provide evidence that the level of cyclic AMP (cAMP) in oocytes regulates oocyte meiotic prophase I and primordial folliculogenesis in the perinatal mouse ovary. Our results show that the early meiotic development of oocytes is closely correlated...

... target gene expression was impaired, Smo-dependent activation of downstream components of the signaling pathway was increased in the absence of Gprk2. This suggests that Gprk2 does indeed play a role in terminating Smo signaling. However, loss of Gprk2 resulted in a decrease in cellular cAMP...

...Leung Kim; Joseph Brzostowski; Amit Majithia; Nam-Sihk Lee; Vanessa McMains; Alan R. Kimmel In Dictyostelium , the interaction of secreted cAMP with specific cell surface receptors regulates the activation/de-activation of GSK3, which mediates developmental cell patterning. In addition...

... signal from the somatic cells is cGMP. Using FRET-based cyclic nucleotide sensors in follicle-enclosed mouse oocytes, we find that cGMP passes through gap junctions into the oocyte,where it inhibits the hydrolysis of cAMP by the phosphodiesterase PDE3A. This inhibition maintains a high concentration...

...-inducing action of DIF-1 but largely refractory to the repressive effect exerted by extracellular cAMP. During normal development, apically situated prestalk cells express the ecmB gene just as they commit themselves to stalk cell differentiation. In the Ax2/gskA– mutant, ecmB is expressed throughout...

... of rickets , which encodes a G-protein coupled receptor in the glycoprotein hormone family that is a putative bursicon receptor. Furthermore, stimulation of components downstream of bursicon, such as a membrane permeant analog of cAMP, or ectopic expression of constitutively active forms of G proteins or PKA...

... active in GalT I-null sperm. Consistent with this, GalT I-null sperm have increased levels of cAMP that correlate closely with both the accelerated kinetics and co-factor-independence of GalT I-null sperm capacitation. By contrast, the kinetics of protein tyrosine phosphorylation and sperm motility...

.... This pharmacological inhibition can be rescued by supplementing with protoporphyrin IX free acid,an activator of sGC, and membrane-permeant cGMP, indicating that NO/cGMP signalling is essential for MG neuron migration. Conversely, the stimulation of the cAMP/protein kinase A signalling cascade results in an inhibition...

... cAMP Oocyte maturation Sea urchin Eggs of most animal species are fertilized by a single sperm and fusion of these two cells is essential for the activation of the developmental program of the zygote. Cases in which multiple sperm fertilize an egg, a condition known as polyspermy...

...Dirk Dormann; Tomoaki Abe; Cornelis J. Weijer; Jeffrey Williams ABSTRACT Dd-STATa, the Dictyostelium STAT (signal transducer and activator of transcription) protein, is selectively localised in the nuclei of a small subset of prestalk cells located in the slug tip. Injection of cAMP...

... form) and basic fibroblast growth factor (bFGF; human) were both from Upstate Biotechnology (Lake Placid, NY). Norepinephrine, isoproterenol, kainic acid, 8Br-cAMP and dibutyryl-cAMP (db-cAMP) were all from Sigma (St Louis, MO). Phenylephrine and clonidine were from Research Biochemicals International...

... regulates cell fate during Dictyostelium development, but in this case it appears to mediate the effects of extracellular cAMP. By direct measurement of GSK-3 kinase activity during Dictyostelium development, we find that there is a rise in activity at the initiation of multicellular development which can...

..., whereas most of the posterior zone consists of prespore cells. We now present evidence that the cAMP-dependent protein kinase (PKA) and the RING domain/leucine zipper protein rZIP interact genetically to mediate a transcriptional activation gradient that regulates the differentiation of prespore cells...

...Rachel Gollop; Alan R. Kimmel ABSTRACT To understand how positional information within an organism specifies patterning during development, we are analyzing spatially regulated gene expression in Dictyostelium . CAR3 is a member of the cAMP, 7-span receptor family which directs the transition from...

...: Centro de Investigaciones Biológicas, c/Veláquez 144, 28006-Madrid, Spain 3 January 1997 © 1997 by Company of Biologists 1997 zinc fingers cAMP SH3 domains trinucleotide repeats Dictyostelium In many developmental systems, cell fate is determined by cellsurface...

... differentiation is induced by Differentiation Inducing Factor (DIF), while spore cell differentiation occurs in response to cAMP. We have discovered the first developmentally regulated Dictyostelium gene, the glycogen phosphorylase gene 2 ( gp2 ) gene, that can be induced by both DIF-1 and cAMP, suggesting...