...)-conjugated rat anti-mouse CD45 (PTPRC) (30-F11, BD Bioscience) on ice for 30-40 minutes, washed, and incubated with allophycocyanin (APC)-conjugated rat anti-mouse c-KIT (2B8, BD Bioscience) on ice for 30-40 minutes. The cells were washed and resuspended in SM containing 3 mg/ml propidium iodide (PI...

... that Endothelin receptor B2 (EDNRB2) and EphB2 are both determinants in this process, and that, unlike in other species, c-KIT is not. We demonstrate that the overexpression of EDNRB2 can maintain normal dorsolateral migration of melanoblasts in the absence of EphB2, and vice versa, suggesting that changes...

.... However, it is not yet clear when, and in what order, crest cells become specified to adopt a particular fate. We report that the receptor tyrosine kinases TrkC and C-Kit are expressed by distinct neural crest subpopulations in vitro. We then analyzed the lineages of individual receptor-expressing crest...

..., such as those dysfunctioned at Steel ( Sl ) locus encoding the c-kit ligand or Dominant white spotting ( W ) locus encoding the receptor c-kit. In the seminiferous tubules of Sl/Sl d or Sl 17H /Sl 17H mice, transplanted donor germ cells proliferated and formed colonies of undifferentiated c-kit...

... corresponds to an orthologue of c- kit . This finding allows us to further elucidate morphogenetic roles for this c- kit -related gene in melanocyte morphogenesis. Our analyses of zebrafish melanocyte development demonstrate that the c- kit orthologue identified in this study is required both for normal...

...Takahiro Kunisada; Hisahiro Yoshida; Hidetoshi Yamazaki; Akitomo Miyamoto; Hiroaki Hemmi; Emi Nishimura; Leonard D. Shultz; Shin-Ichi Nishikawa; Shin-Ichi Hayashi ABSTRACT Mutations at the murine dominant white spotting ( Kit W ) and steel (Mgf Sl ) loci, encoding c-Kit receptor kinase and its...

...Claudio Sette; Arturo Bevilacqua; Andrea Bianchini; Franco Mangia; Raffaele Geremia; Pellegrino Rossi ABSTRACT A truncated form of the c-kit tyrosine kinase receptor, cor-responding to the phosphotransferase portion of the cyto-plasmic catalytic domain and the carboxyterminus (tr-kit...

...Hisahiro Yoshida; Takahiro Kunisada; Moriaki Kusakabe; Satomi Nishikawa; Shin-Ichi Nishikawa ABSTRACT The injection of an antagonistic anti-murine c -kit mono-clonal antibody ACK2 during mouse embryonic development produced three distinctive pigmentation patterns on the coat of the offspring...

...Cristina Albanesi; Raffaele Geremia; Marco Giorgio; Susanna Dolci; Claudio Sette; Pellegrino Rossi ABSTRACT In the postnatal testis, the c- kit transmembrane tyrosine-kinase receptor is expressed in type A spermatogonia, and its transcription ceases at the meiotic phase of spermato-genesis...

... of crest-derived cells remaining in the migration staging area expresses mRNAs for the receptor tyrosine kinase, c-kit, and tyrosinase-related protein-2, both of which are characteristic of melanocyte precursors. These putative melanocyte precursors are subsequently observed on the lateral crest migration...

...Minetaro Ogawa; Satomi Nishikawa; Kazuya Yoshinaga; Shin-Ichi Hayashi; Takahiro Kunisada; Junji Nakao; Tatsuo Kina; Tetsuo Sudo; Hiroaki Kodama; Shin-Ichi Nishikawa ABSTRACT The protooncogene c- kit encodes a receptor type tyro-sine kinase and is allelic with the W locus of mice. SLF, the c-Kit...

...Hitomi Maeda; Akihiro Yamagata; Satomi Nishikawa; Kazuya Yoshinaga; Shigeru Kobayashi; Katsuhide Nishi; Shin-Ichi Nishikawa ABSTRACT A discovery that the protooncogene encoding the receptor tyrosine kinase, c -kit , is allelic with the Dominant white spotting (W) locus establishes that c- kit plays...

...Osamu Ohneda; Nobuaki Yanai; Masuo Obinata ABSTRACT Mutations at the murine dominant-white spotting locus (W) (c-Jtir) affect various aspects of hematopoiesis. We have made antibodies against c-Kit with the synthetic peptides deduced from the murine c-kit gene and examined the role of c-Kit...

... nature of W and Sl mutations, respectively, it has recently been shown that W encodes the c-kit receptor tyrosine kinase while Sl encodes a ligand for this receptor. Previous in situ hybridization analysis has shown that both c-kit and steel are expressed in the embryo in anatomical sites known...

...Kazuya Yoshinaga; Satomi Nishikawa; Minetaro Ogawa; Shin-Ichi Hayashi; Takahiro Kunisada; Toyoaki Fujimoto; Shin-Ichi Nishikawa ABSTRACT Recent studies have shown that the dominant white spotting (W) locus encodes the proto-oncogene c-kit , a member of the tyrosine kinase receptor family. One...

...Katya Manova; Karl Nocka; Peter Besmer; Rosemary F. Bachvarova Recently, it has been shown that the c-kit proto-oncogene is encoded at the white spotting ( W) locus in mice. Mutations of this gene cause depletion of germ cells, some hematopoietic cells and melanocytes. In order to define further...