... in mouse blastocysts – both canonical and alternatively processed mRNAs – including those encoding cell cycle arrest and apoptosis factors, raising the possibility that NMD is essential for embryonic cell proliferation and survival. In support, the inner cell mass of Upf2 -null blastocysts rapidly...

... sites that permit implantation. When translating systems biology of the undifferentiated bovine trophectoderm, we uncovered that inhibition of RhoA/Rock promoted self-renewing proliferation and substantially increased blastocyst size. Analysis of transcripts suppressed by Rock inhibition revealed...

... proteins, which might not recapitulate endogenous protein activities. Here, we present two strategies for higher throughput production of endogenous protein reporters in mice, focusing on the blastocyst model of development. Our first strategy makes use of a split fluorescent protein, mNeonGreen2 (mNG2...

...Berenika Płusa; Anna Piliszek ABSTRACT Pre-implantation mammalian development unites extreme plasticity with a robust outcome: the formation of a blastocyst, an organised multi-layered structure ready for implantation. The process of blastocyst formation is one of the best-known examples of self...

... for trophectodermal Tat-Cre/loxP recombination. We used the natural permeability barrier in mouse blastocysts in combination with off-the-shelf Tat-Cre recombinase to achieve editing of conditional alleles in the trophoblast lineage. This direct approach enables gene function analysis during implantation...

.../ Highlighted Article: During primitive endoderm differentiation in mouse, ERK activation is regulated by NANOG, which induces the expression of both Fgf4 and Etv5 , providing intrinsic and extrinsic signals at the same time. Mouse Blastocyst Cell differentiation Signalling between cells...

... of primitive endoderm identity is conserved in primates. Unexpectedly, OTX2 is also associated with primitive endoderm specification in human and non-human primate blastocysts. Our cross-species analysis demarcates both conserved and primate-specific features of preimplantation development, and underscores...

...Xianle Shi; Zixi Yin; Bin Ling; Lingling Wang; Chang Liu; Xianhui Ruan; Weiyu Zhang; Lingyi Chen The Hippo pathway modulates the transcriptional activity of Yap to regulate the differentiation of the inner cell mass (ICM) and the trophectoderm (TE) in blastocysts. Yet how Hippo signaling...

... specification in rabbit more closely resembling that of human and primate embryos. Blastocyst Epiblast FGF Primitive endoderm Rabbit Preimplantation development in the mammalian embryo is characterised by two consecutive cell fate specification events. During the first, cells located...

...Marilyn B. Renfree; Jane C. Fenelon Embryonic diapause – a period of embryonic suspension at the blastocyst stage – is a fascinating phenomenon that occurs in over 130 species of mammals, ranging from bears and badgers to mice and marsupials. It might even occur in humans. During diapause...

... in their architecture in preparation for embryo implantation. Implantation Receptivity Uterus Blastocyst Embryo Confocal Imaging Surface curvature Wnt5a The uterus is the prerequisite for propagating mammalian species, as implantation in this tissue establishes pregnancy and protects the developing...

... by The Company of Biologists Ltd 2016 Summary: Generation of a new targeted Ctnnb1 allele shows that maternal/zygotic Ctnnb1 null embryos cannot complete preimplantation development due to defective blastomere adhesion. β-catenin allele Ctnnb1 tm1Knw Blastocyst Preimplantation Peri...

... populations reminiscent of the blastocyst, identifying the target cells of LIF is necessary to understand the propagation of pluripotency. We recently found that LIF acts under self-renewing conditions to stimulate the fraction of ESCs that express extraembryonic markers, but has little impact on pluripotent...

... how the chromatin remodelling protein CHD4 facilitates the first lineage decision in mammalian embryogenesis. Embryos lacking CHD4 can form a morphologically normal early blastocyst, but are unable to successfully complete the first lineage decision and form functional trophectoderm (TE...

... and maintenance of embryonic stem cells and for reprogramming somatic cells to pluripotency. In vivo , it prevents the ectopic differentiation of early embryos into trophoblast. Here, we further explore the role of Oct4 in blastocyst formation and specification of epiblast versus primitive endoderm lineages using...

...Kotaro J. Kaneko; Melvin L. DePamphilis It has been suggested that during mouse preimplantation development, the zygotically expressed transcription factor TEAD4 is essential for specification of the trophectoderm lineage required for producing a blastocyst. Here we show that blastocysts can form...

... implantation or blastocyst lineage specification. We have previously shown that mice generated by aggregation of Porcn mutant ESCs with tetraploid embryos die at gastrulation stages, making it impossible to generate a viable mouse line ( Biechele et al., 2011 ; Cox et al., 2010 ). In order...

...Minjung Kang; Anna Piliszek; Jérôme Artus; Anna-Katerina Hadjantonakis The emergence of pluripotent epiblast (EPI) and primitive endoderm (PrE) lineages within the inner cell mass (ICM) of the mouse blastocyst involves initial co-expression of lineage-associated markers followed by mutual exclusion...

... F. , Galloway J. , Sequerra R. , Kasper J. , Ayala R. , Stewart A. F. , Dymecki S. M. ( 2000 ). High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP . Nat. Genet. 25 , 139 - 140 . Rossant J. , Tam P. P. ( 2009 ). Blastocyst...

... proposed that mouse pre-implantation patterning may be a result of stochastic processes and that, although some cells have begun to express markers of different lineages, cell fate is not determined until the early blastocyst stage ( Motosugi et al., 2005 ; Dietrich and Hiiragi, 2007 ; Ralston...