... embryonic germ cells (EGCs) in culture in the presence of particular extracellular factors, such as combinations of Steel factor (KITL), LIF and bFGF (FGF2). Early PGCs form EGCs more readily than do later PGCs, and PGCs lose the ability to form EGCs by embryonic day (E) 15.5. Here, we examined the effects...

... and secondary notochord. Conversely, primary notochord precursors isolated prior to induction formed notochord in presence of BMP-4 protein. While bFGF protein had a similar activity, notochord precursors showed a different time window of competence to respond to BMP-4 and bFGF. Our data are consistent...

..., respectively, indicating that the type of tissue induced depends on differences in the responsiveness of the signal-receiving blastomeres. Basic fibroblast growth factor (bFGF), but not activin A, induced formation of mesenchyme cells as well as notochord cells. Treatment of mesenchyme-muscle precursors...

... ), a receptor for basic fibroblast growth factor (bFGF), as well as scl , a transcription factor, for their potential to generate BL-CFCs and Flk1 + cells, to further define events leading to hemangioblast development. Our data suggest that bFGF-mediated signaling is critical for the proliferation...

... ) † Present address: United Medical and Dental School (UMDS), Developmental Neurobiology, Guy’s Hospital, London Bridge, SE1 9RT London, UK 07 01 1997 © 1997 by Company of Biologists 1997 bFGF Engrailed mid-/hindbrain junction Protease nexin-1 transcription mouse Induction...

... expression in the posterior paraxial mesoderm and ventral blastopore, and its exclusion from the most dorsal mesodermal structure, the notochord. Antipodean is induced by several molecules of the TGF-β class, but in contrast to Xbrachyury , not by bFGF. This result suggests that the expression of these T-box...

..., activins and basic fibroblast growth factor (bFGF). In the ascidian, Halocynthia roretzi , treatment with bFGF of presumptive-notochord blastomeres that had been isolated at the early 32-cell stage promoted the formation of notochord at a low concentration of bFGF (0.02 ng/ml), while activin failed...

... axial tissue induces midbrain and hindbrain fates from prospective forebrain. The growth factor bFGF mimics the effect of the posterior dorsal explant in that it (i) induces forebrain to express hindbrain markers, (ii) induces prospective hindbrain explants to make spinal cord, but not forebrain...

... cord. Here we present evidence that bFGF can mimic the organizer action by inducing Xenopus ectoderm cells in culture to express four position-specific neural markers ( XeNK-2, En-2, XlHbox1 and XlHbox6 ) along the anteroposterior axis. bFGF also induced the expression of a general neural marker NCAM...

... with bFGF and noggin express Xslu but not NCAM although the mesoderm marker Xbra is also expressed. Explants treated with noggin alone express NCAM only. An indication that induction of the neural plate border is regulated independently of the neural plate is obtained from experiments using ultraviolet...

... that cultured explants from the posterior marginal zone at stages XI to XIII (consisting of the posterior marginal zone and part of Koller’s sickle) have a high propensity to form haemoglobin (Hb), which could be inhibited at stage XI by adding antibody against basic fibroblast growth factor (bFGF...

...Elizabeth Noll; Robert H. Miller ABSTRACT During development, oligodendrocyte precursors undergo sequential stages of differentiation characterized by expression of distinct cell surface properties and proliferative responses. Although both PDGF and bFGF are mitogenic for these cells, the factors...

... in cultured periotic mesenchyme. In this study, we provide evidence that basic fibroblast growth factor (bFGF) can elicit a specific but limited chondrogenic response in cultured periotic mesenchymal cells. We also demonstrate that simultaneous addition of bFGF and TGF-β 1 to cultured periotic mesenchyme...

... signal, to initially establish the dorsal-ventral axis of the mesoderm. The Xenopus bFGF protein is encoded by a 4.5 kb mRNA transcribed during oogenesis and again after the gastrula stage during embryogenesis ( Kimelman et al., 1988 ). Four small open reading frames precede the initiation codon...

...Jonathan Cooke; Adeline Wong ABSTRACT Xenopus and murine activin A homologues (XTC-MIF and WEHI-MIF) and Xenopus and bovine basic fibroblast growth factor (bFGFs) are potent inducers of mesodermal and endodermal pathways of development in amphibian blastular animal cap cells. Porcine transforming...

... supplied growth factors and hormones. It was found that the corneal endothelial cells could be stimulated to initiate DNA synthesis by exposure to basic fibroblast growth factor (bFGF). The thymidine label ling index nearly doubled after bFGF addition. Northern blot analysis revealed the presence of bFGF...

... proliferation and cell loss from the culture. The addition of basic fibroblast growth factor (bFGF) to defined medium leads to an increase in cell number in the frontonasal mass, while the cell number of mandibular and maxillary cultures is relatively unaffected. The percentage of cells in S-phase is highest...

...Chaya Kalcheim; Gera Neufeld ABSTRACT Basic fibroblast growth factor (bFGF) promotes the survival of a subpopulation of non-neuronal cells developing from trunk neural crest (Kalcheim, Devi Biol. 134, 1-10, 1989). It was therefore important to determine whether this factor is present in the nervous...

... stage 10, and perhaps until stage 10.5, this suggests that bFGF cannot be the sole inducer of mesoderm in vivo . Taken together, these results are consistent with XTC-MIF being a dorsoanterior inducer and XbFGF a ventroposterior inducer, suggesting that body pattern is established by the interaction...

... 1989 organiser body pattern XTC-MIF bFGF dorsal blastoporal lip lineage labelling Xenopus Spemann and Mangold (1924) first described the graft, from the dorsal blastoporal lip of an early newt gastrula into the presumptive ventral region of a similar host, that led to formation...