... or not present in animal embryos. Because little is known about S/M checkpoints in plant cells and embryos, we have investigated the effect of aphidicolin, a specific inhibitor of DNA polymerases α and δ, on cell division and morphogenesis in Fucus and Pelvetia zygotes. Both DNA replication and cell division...

...Martha B. Rollins; Matthew T. Andrews ABSTRACT Xenopus embryos were transferred into media containing aphidicolin at late blastula, mid-gastrula, and early neurula stages. In each case, embryos continued to differentiate in the absence of DNA replication. When the inhibitor was added at late...

... studies revealed that a dose of 1000 rad reduced DNA synthesis of mid and late 12-day glands by 72 and 65 %, respectively. Histological examination of X-irradiated late 12-day gland showed that mitotic figures were rarely seen in the epithelium at 6 h of culture. Aphidicolin, a specific inhibitor of DNA...

...Roger K. W. Smith; Martin H. Johnson ABSTRACT The effects of aphidicolin, a reversible inhibitor of DNA polymerase alpha, both on replication and on development of the mouse embryo from the 2- and 4-cell stages to the compacted late 8-cell stage have been assessed. The continuous presence...