... ( han , hand2 ) acerebellar Fibroblast growth factors fgf8 Fate mapping Heart The endoderm gives rise to pharynx and intestine, and also to thyroid,lung, pancreas and liver. Development of these organs is initiated in different areas of the primitive gut, where, after induction...

... Cartilage Neural crest Segmentation Fgf8 Fgf3 acerebellar GFP Zebrafish The cartilages and bones that form the skeleton of the face and, in mammals, the middle ear, are derived from a specialized population of ectomesenchyme, the cranial neural crest( Le Douarin, 1982 ; Weston et al., 2004...

...József Jászai; Frank Reifers; Alexander Picker; Tobias Langenberg; Michael Brand In zebrafish acerebellar ( ace ) embryos, because of a point mutation in fgf8 , the isthmic constriction containing the midbrain-hindbrain boundary (MHB) organizer fails to form. The mutants lack cerebellar...

..., Fgf8 appears to be the principal ligand required for mesodermal development, as mouse Fgf8 mutants do not form mesoderm. In zebrafish, Fgf8 is encoded by the acerebellar locus, and, similar to its mouse otholog, is expressed in early mesodermal precursors during gastrulation. However, zebrafish fgf8...

... fate. By comparing GFP protein and her5 transcription, we further reveal the spatiotemporal dynamics of her5 expression that conditions neurogenesis progression towards the MHB over time. Finally, we trace the molecular identity of GFP-positive cells in the acerebellar ( ace ) and no-isthmus ( noi...

... FGF8 acerebellar valentino krox-20 Zebrafish Embryonic organizing centers, specialized groups of cells that are both necessary and sufficient to pattern a larger cell population to which they belong ( Struhl and Basler, 1993 ; Spemann and Mangold, 1924 ), have been shown to play...

... expression are not known. We describe here that the secreted signaling factor Fgf8 is expressed in and required for development of the zebrafish heart precursors, particularly during initiation of cardiac gene expression. fgf8 is mutated in acerebellar ( ace ) mutants, and homozygous mutant embryos do...

... organizer in acerebellar mutants, which lack a MHB and cerebellum, but retain a tectum, and are mutant for fgf8 , a candidate inducer and polarizer. We examine the retinotectal projection in the mutants to assay polarity in the tectum. In mutant tecta, retinal ganglion cell (RGC) axons form overlapping...

... 1999 © 1999 by Company of Biologists 1999 Neurogenesis Forebrain Optic stalk fgf8 pax genes acerebellar noisthmus Zebrafish Danio rerio During early vertebrate development, the nervous system becomes subdivided into discrete regions along its anterior- posterior...

... the structure of the Fgf8 gene, we show that acerebellar is a zebrafish Fgf8 mutation that may inactivate Fgf8 function. Homozygous acerebellar embryos lack a cerebellum and the midbrain-hindbrain boundary organizer. Fgf8 function is required to maintain, but not initiate, expression of Pax2 . 1 and other...

... Mutations in two genes affect the formation of the boundary between midbrain and hindbrain (MHB): no isthmus ( noi ) and acerebellar ( ace ). noi mutant embryos lack the MHB constriction, the cerebellum and optic tectum, as well as the pronephric duct. Analysis of noi mutant embryos with neuron-specific...