... for renal cell fate choice and ciliogenesis in zebrafish. esrrγa deficiency altered proximodistal nephron patterning, decreased the multiciliated cell populace and disrupted ciliogenesis in the nephron, Kupffer's vesicle and otic vesicle. These phenotypes were consistent with interruptions in prostaglandin...

... always lead to early embryonic lethality, but zebrafish lines with RBC deficiencies can survive to larval stage. By taking advantage of zebrafish model, we find that the survival of nascent HSPCs is impaired in alas2 - or alad -deficient embryos with aberrant heme biosynthesis in RBCs. Heme-deficient...

.... The canonical eIF4E1A is required for cell viability, yet other related eIF4E families exist and are utilized in specific contexts or tissues. Here, we describe a family called Eif4e1c for which we find roles during heart development and regeneration in zebrafish. The Eif4e1c family is present in all aquatic...

...Lili Zhou; Anthony R. McAdow; Hunter Yamada; Brooke Burris; Dana Klatt Shaw; Kelsey Oonk; Kenneth D. Poss; Mayssa H. Mokalled ABSTRACT Unlike mammals, adult zebrafish undergo spontaneous recovery after major spinal cord injury. Whereas reactive gliosis presents a roadblock for mammalian spinal cord...

... signaling cues and thyroid transcription factors remained elusive. By analyzing Pax2a and Nkx2.4b expression dynamics in relation to endodermal FGF and BMP signaling activities in zebrafish embryos, we identified a Pax2a-expressing thyroid progenitor population that shows enhanced FGF signaling but lacks...

... cellular cohorts interact with an active epithelium. In zebrafish neuromasts, nascent sibling hair cells invert positions by rotating ≤180° around their geometric center after acquiring different identities via Notch1a-mediated asymmetric repression of Emx2. Here, we show that this multicellular rotation...

... cells. In zebrafish, the transcription factor npas4l ( cloche ) is required for the specification of hemato-vascular progenitor cells. However, it is unclear whether npas4l is the sole factor at the top of the hemato-vascular specification cascade. Here, we show that arnt1 and arnt2 genes are required...

... signaling pathway genes cause human skeletal diseases such as Robinow syndrome and Brachydactyly Type B1, which disrupt bone growth throughout the endochondral skeleton. Ror2 is one of several non-canonical Wnt receptor/co-receptors. Here, we show that ror2 −/− mutant zebrafish have craniofacial skeletal...

...; Nicolas Rohner; C. Ben Lovely; Kristen M. Kwan; Alexa Burger; Christian Mosimann ABSTRACT Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Gateway-compatible vector collections has transformed zebrafish transgenesis with an accessible modular system. Here...

... of the vasculature. We therefore performed a longitudinal analysis of zebrafish caudal fin vascular assembly, revealing the existence of temporally and spatially distinct morphogenetic processes. Initially, vein-derived endothelial cells (ECs) generated arteries in a reiterative process requiring vascular...

...-dependency and function of her genes in the thalamic proliferation zone of zebrafish larvae. Nine Notch-dependent genes, her2 , her4.1-4.5 , her12 , her15.1-15.2 , and two Notch-independent genes, her6 and her9 , are differentially expressed and define distinct NSC and progenitor populations. her6...

... of such cells during β-cell regeneration is currently unknown. Here, using a zebrafish nitroreductase (NTR)-mediated β-cell ablation model, we reveal that ghrelin-positive ε-cells in the pancreas act as contributors to neogenic β-cells after extreme β-cell loss. Further studies show that the overexpression...

... cardiac regeneration. Here, we report that the forkhead transcription factor Foxm1 is required for cardiomyocyte proliferation after injury through transcriptional regulation of cell cycle genes. Transcriptomic analysis of injured zebrafish hearts revealed that foxm1 expression is increased in border zone...

...Sreelaja Nair; Elaine L. Welch; Cara E. Moravec; Ryan L. Trevena; Christina L. Hansen; Francisco Pelegri ABSTRACT We show that the zebrafish maternal-effect mutation too much information ( tmi ) corresponds to zebrafish prc1-like ( prc1l ), which encodes a member of the MAP65/Ase1/PRC1 family...

...) regional apicobasal vertical-to-horizontal alignment. Using GliaMorph, we quantified MG development on a global and single-cell level, showing increased feature elaboration and subcellular morphological rearrangement in the zebrafish retina. As proof of principle, we analysed expression changes in a mouse...

... epithelium. (B) Timelines of eye development in different vertebrate species. (C) Graph representing the optic cup volume relative to that of zebrafish in the different depicted species: (from the right) human, mouse, chicken, medaka and zebrafish. The correct execution of all these morphogenetic...

... zebrafish primordial germ cells that migrate throughout the developing embryo. When migrating within an ectodermal environment, the germ cells form fewer and smaller blebs when compared with their behavior within mesodermal environment. We find that cortical tension of neighboring cells is a parameter...

...Amey Y. Rayrikar; Ganesh A. Wagh; Manas K. Santra; Chinmoy Patra ABSTRACT Intervertebral disc (IVD) degeneration is the primary cause of back pain in humans. However, the cellular and molecular pathogenesis of IVD degeneration is poorly understood. This study shows that zebrafish IVDs possess...

... mutants of nanog (M nanog ) in zebrafish, we demonstrated that Nanog tightly controls translation of maternal mRNA during oogenesis via transcriptional repression of eukaryotic translation elongation factor 1 alpha 1, like 2 ( eef1a1l2 ). Loss of maternal Nanog led to defects of egg maturation, increased...

... of hematopoietic progenitor cell fate is essential for the development of adequate blood cell subpopulations, although regulators of cell fate commitment have not been fully defined. Here, we show that primary erythropoiesis and myelopoiesis in zebrafish embryos are synergistically regulated by blf and the drl...