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Keywords: ZO-1
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Journal Articles
Journal: Development
Development (2011) 138 (19): 4155–4166.
Published: 1 October 2011
... Bank, 1:10), and with polyclonal antibodies to ZO-1 (Zymed 40-2200, 1:100), aPKC (Santa Cruz sc-216, 1:60), laminin (Sigma L9393, 1:100), LGN (Sigma HPA007327, 1:100), MyoD [from B. Paterson (NIH, Bethesda, MD, USA), 1:1000], and γ-tubulin (Sigma T3559, 1:500). Secondary antibodies were coupled to Cy2...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (2002) 129 (23): 5323–5337.
Published: 1 December 2002
... the typical polarized and localized distribution of E-cadherin and ZO-1 and lacked β-tubulin accumulation. Polarized epithelial cells interact with each other through specialized junctional complexes ( Farquhar and Palade, 1963 ). Junctional complexes and microtubules are involved not only in maintaining...
Journal Articles
Journal: Development
Development (1997) 124 (10): 2027–2037.
Published: 15 May 1997
... that individual components of the tight junction assemble in a temporal sequence, with membrane assembly of the cytoplasmic plaque protein ZO-1 occurring 12 hours before that of cingulin. Subsequently, two alternatively spliced isoforms of ZO-1 (α + and α − ), differing in the presence or absence of an 80 residue...
Journal Articles
Journal: Development
Development (1993) 117 (3): 1135–1144.
Published: 1 March 1993
..., although earlier assembly occurs in a minority (up to 20%) of specimens. Cingulin assembly at the tight junction is sensitive to cycloheximide and is identifiable approx. 10 hours after cell adhesion is initiated and ZO-1 protein assembles. Collectively, our results indicate that (i) cingulin from...
Journal Articles
Journal: Development
Development (1992) 116 (Supplement): 105–112.
Published: 1 April 1992
... this period, initial focal junction sites gradually extend laterally to become zonular and acquire the peripheral tight junction proteins ZO-1 and cingulin. Our studies indicate that junction components assemble in a temporal sequence with ZO-1 assembly preceding that of cingulin, suggesting that the junction...