... hypothesis on X-chromosome inactivation in mammals and the 30th anniversary of XIST/Xist discovery, the meeting had to be postponed because of the COVID-19 pandemic. Seven years after the latest XCI meeting in London, the enthusiasm and expectations of the community were at their highest, bringing together...

... targeted the TAD neighboring that of the noncoding transcript Xist , which controls X-chromosome inactivation. Inverting 245 kb within the TAD led to expected rearrangement of CTCF-based contacts but revealed heterogeneity in the ‘contact’ potential of different CTCF sites. Expression of most genes therein...

...Andreas Postlmayr; Charles Etienne Dumeau; Anton Wutz ABSTRACT We previously identified the cyclin dependent kinase Cdk8 as a putative silencing factor for Xist . To investigate its role in X inactivation, we engineered a Cdk8 mutation in mouse embryonic stem cells (ESCs) carrying an inducible...

... current knowledge of how X chromosome inactivation, an important model for epigenetic gene regulation, is initiated and controlled during early human embryo development. X chromosome inactivation Human Germline Embryo XIST XACT Pluripotent stem cells Université de Recherche Paris...

...Atsushi Fukuda; Atsushi Mitani; Toshiyuki Miyashita; Akihiro Umezawa; Hidenori Akutsu Repression of maternal Xist (Xm- Xist ) during preimplantation in mouse embryos is essential for establishing imprinted X chromosome inactivation. Nuclear transplantation (NT) studies using nuclei derived from non...

.... , Achame E. M. , Ghazvini M. , Boers R. , Kenter A. , Rentmeester E. , Grootegoed J. A. , Gribnau J. ( 2011 ). RNF12 activates Xist and is essential for X chromosome inactivation . PLoS Genet. 7 , e1002001 . Borsani G. , Tonlorenzi R. , Simmler M...

... X chromosomes in order to balance X-linked gene dosage with their male counterparts. The process of XCI is dependent upon the long non-coding RNA Xist , which is expressed from and coats the inactivated X chromosome (Xi) in cis. During mouse embryogenesis, imprinted XCI inactivates the paternally...

... inactivation Xist X chromosome X-chromosome inactivation (XCI) is the process that has evolved in mammals to equalise the dosage of X-linked genes in XX females relative to XY males. Cells of early XX mammalian embryos silence a single X chromosome. Once established, chromosome silencing...

... The authors declare no competing financial interests. © 2011. X chromosome X-inactivation hypothesis Epigenetics Xist X-chromosome inactivation (XCI) refers to the silencing of one of the two X chromosomes in females during early development, thus ensuring dosage compensation...

... inactivation is regulated by a cis-acting master switch, the Xist locus, the product of which is a large non-coding RNA that coats the chromosome from which it is transcribed, triggering recruitment of chromatin modifying factors that establish and maintain gene silencing chromosome wide. Chromosome coating...

...Yuko Hoki; Naomi Kimura; Minako Kanbayashi; Yuko Amakawa; Tatsuya Ohhata; Hiroyuki Sasaki; Takashi Sado X-inactivation in female mammals is triggered by the association of non-coding Xist RNA in cis with the X chromosome. Although it has been suggested that the A-repeat located in the proximal part...

...Takashi Sado; Yuko Hoki; Hiroyuki Sasaki Dosage differences of X-linked genes between male and female mammals are compensated for by a mechanism known as X-inactivation, and the noncoding Xist gene plays a crucial role in this process. The expression of Xist is regulated in cis by its noncoding...

... and Xist while in the embryo proper imprinted expression for the most part was preserved. Loss of imprinted expression was associated with a decrease in methylation at the H19 and Snrpn imprinting control regions. These results indicate that tissues of trophectoderm origin are unable to restore genomic...

...Takashi Sado; Masaki Okano; En Li; Hiroyuki Sasaki Xist (X-inactive specific transcript) plays a crucial role in X-inactivation. This non-coding RNA becomes upregulated on the X chromosome that is to be inactivated upon differentiation. Previous studies have revealed that although maintenance-type...

...Takashi Sado; Zhenjuan Wang; Hiroyuki Sasaki; En Li ABSTRACT In mammals, X-chromosome inactivation is imprinted in the extra-embryonic lineages with paternal X chromosome being preferentially inactivated. In this study, we investigate the role of Tsix , the antisense transcript from the Xist locus...

...Carolyn H. Buzin; Jeffrey R. Mann; Judith Singer-Sam ABSTRACT We have investigated expression of the Xist gene in mouse female adult kidney, embryos and embryonic stem (ES) cells undergoing in vitro differentiation as embryoid bodies. Using the quantitative RT-PCR single nucleotide primer extension...