... Skeleton Wnt Sea urchin National Science Foundation http://dx.doi.org/10.13039/100000001 IOS 1656752 The movement of ions is responsible for regulating processes such as wound healing, regeneration and development ( Adams and Levin, 2006 ; Adams et al., 2007 ; Akasaka et al., 1997...

... + UM. Moreover, precocious and enhanced canonical WNT signaling led to cellular and molecular changes that mimicked the loss of Tbx18 in this domain: Dach1 , Fzd10, Pla2g7 and Wnt4 were ectopically activated, while UM-specific genes ( Sox9 , Ptch1 and Bmp4 ) were lost; the UM was highly...

... show that hypoxia-induced T expression enables symmetry breaking and axial elongation in the absence of exogenous WNT activation. When combined with exogenous WNT activation, hypoxia enhances lineage representation in gastruloids, as demonstrated by highly enriched signatures of gut endoderm, notochord...

...Keagan Dunville; Fabrizio Tonelli; Elena Novelli; Azzurra Codino; Verediana Massa; Anna Maria Frontino; Silvia Galfrè; Francesca Biondi; Stefano Gustincich; Matteo Caleo; Luca Pandolfini; Claudia Alia; Federico Cremisi ABSTRACT Using the timely re-activation of WNT signalling in neuralizing human...

.... Conditional inactivation of the Wnt secretion protein Wls in Pax3-expressing lineage cells disrupted frontonasal primordial patterning, cell survival and directional outgrowth, resulting in altered facial structures, including midfacial hypoplasia and midline facial clefts. Single-cell RNA sequencing revealed...

... with that of diffusion of locally expressed Wnt ligands, suggesting a cooperative relationship between these two PCP regulators. Next, we considered the possibility that the tension-induced change in cell shape might act as a cue for PCP. When a tissue is stretched uniaxially, the cells also elongate in the same...

...Nicolle A. Bonar; David I. Gittin; Christian P. Petersen ABSTRACT Tissue identity determination is crucial for regeneration, and the planarian anteroposterior (AP) axis uses positional control genes expressed from body wall muscle to determine body regionalization. Canonical Wnt signaling...

... of adhesion, including activation of latent-TGFβs in the ECM. Quantitative sequencing of the brain endothelial cell transcriptome identifies TGFβ-regulated genes with putative links to blood vessel morphogenesis, including several genes linked to Wnt/β-catenin signaling. These results reveal that the β8...

...Soumyashree Das; Qiang Feng; Iyshwarya Balasubramanian; Xiang Lin; Haoran Liu; Oscar Pellón-Cardenas; Shiyan Yu; Xiao Zhang; Yue Liu; Zhi Wei; Edward M. Bonder; Michael P. Verzi; Wei Hsu; Lanjing Zhang; Timothy C. Wang; Nan Gao ABSTRACT Although Wnt signaling is clearly important for the intestinal...

... mechanisms underlying the epithelial-mesenchymal interaction during alveologenesis are poorly understood. Here, we report that Wnts produced by epithelial cells are crucial for neonatal alveologenesis. Deletion of the Wnt chaperone protein Wntless homolog (Wls) disrupts alveolar formation, resulting...

... to overproliferation of interstitial cells regionally in the kidney medulla. Analysis of markers for BMP and TGFβ pathway activation reveals that loss of Smad4 primarily reduces TGFβ signaling in the interstitium. Whereas TGFβ signaling is reduced in these cells, marker analysis shows that Wnt/β-catenin signaling...

... quiescent and are stimulated by niche signals to activate and produce neurons. Wnt/β-catenin signalling acts at different steps along the hippocampal neurogenic lineage, but whether it has a direct role in the regulation of NSCs remains unclear. Here, we used Wnt/β-catenin reporters and transcriptomic data...

...), that is required for the progression of tooth development. IK cell dynamics demonstrate the hallmarks of a signaling center: cell cycle exit, condensation and eventual silencing through apoptosis. IK initiation and maturation are defined by the juxtaposition of cells with high Wnt activity to Shh -expressing non...

..., is identified as a master gene in hindbrain choroid plexus patterning and multiciliogenesis. Choroid plexus Patterning Development Hindbrain Wnt BMP Cilium Multiciliated cells Multiciliated epithelium Microvilli Zfp423 ZNF423 Joubert syndrome JS19 Hydrocephalus The choroid plexus...

.... Wnt proteins are evolutionarily conserved polarity cues, yet Wnt mutants display variable PCP defects; thus, how Wnts regulate PCP remains unresolved. Here, we have used the developing cochlea as a model system to show that secreted Wnts regulate PCP through polarizing a specific subset of PCP...

... give rise to zymogenic cells in culture. Modulating the activity of the WNT, BMP and Notch pathways in vivo and ex vivo , we found that WNTs are essential for the maintenance of Lgr5 + epithelial cells. Notch prevents differentiation of the embryonic epithelial cells along all secretory lineages...

...Olga Ossipova; Keiji Itoh; Aurelian Radu; Jerome Ezan; Sergei Y. Sokol ABSTRACT Among the three embryonic germ layers, the mesoderm plays a central role in the establishment of the vertebrate body plan. The mesoderm is specified by secreted signaling proteins from the FGF, Nodal, BMP and Wnt...

... pathway, that increases Wnt production from motor neurons. We also provide evidence that this neuronal Wnt contributes to EGL-30-stimulated vulval development, with muscle producing a parallel developmental signal. As diverse sensory modalities stimulate motor neurons via acetylcholine, this mechanism...

... molecular signals ensure ovary versus testis differentiation with canonical Wnt/β-catenin signals paramount for promoting the ovary pathway. Notably, few direct downstream targets have been identified. We report that Wnt/β-catenin signaling directly stimulates Irx3 and Irx5 transcription in the developing...

... are an excellent model for investigating how signaling control facilitates the generation of defined neuronal populations. Wnt signaling is required for habenular neuron type specification, asymmetry and axonal connectivity. The temporal regulation of this pathway and the players involved have, however, remained...