... kinase, Wee1/Myt1, on Thr14 (T14) and Tyr15 (Y15). This triply phosphorylated Cdk1 is activated at the G2/M transition by the dephosphorylation of T14 and Y15 by the dual-specificity phosphatase Cdc25. Likewise, the kinases and phosphatases that regulate Cdk1 are also subject to positive and negative...

...Zhigang Jin; Ellen M. Homola; Philip Goldbach; YunHee Choi; Julie A. Brill; Shelagh D. Campbell The metazoan Wee1-like kinases Wee1 and Myt1 regulate the essential mitotic regulator Cdk1 by inhibitory phosphorylation. This regulatory mechanism, which prevents Cdk1 from triggering premature mitotic...

... for correspondence (e-mail: [email protected] ) * Present address: Abbott Laboratories; Abbott Park, IL 60064, USA 22 12 2003 © 2004. 2004 Xenopus Wee1 Wee2 Cdk Cell cycle Morphogenesis Convergent extension Somitogenesis During embryogenesis, there must...

... mitotic cell cycle, the mid-blastula transition (MBT) and at gastrulation. In this report, we investigate the contribution of maternal Wee1, a kinase inhibitor of CyclinB/Cdc2, to these crucial developmental transitions. By depleting Wee1 protein levels using antisense morpholino oligonucleotides, we show...

...Monica S. Murakami; George F. Vande Woude ABSTRACT In Xenopus , cdc2 tyrosine phosphorylation is detected in the first 60-75 minute cell cycle but not in the next eleven cell cycles (cycles 2-12) which are only 30 minutes long. Here we report that the wee1/cdc25 ratio increases before the first...