...Marina Ramiro-Pareta; Claudia Müller-Sánchez; Rosa Portella-Fortuny; Carolina Soler-Botija; Alejo Torres-Cano; Anna Esteve-Codina; Antoni Bayés-Genís; Manuel Reina; Francesc X. Soriano; Eloi Montanez; Ofelia M. Martínez-Estrada ABSTRACT Wt1 encodes a zinc finger protein that is crucial...

... of the ureter. Using misexpression and loss-of-function mice combined with molecular profiling approaches, we show that Tbx18 is required and sufficient to repress metanephric mesenchymal gene programs. We identify Wt1 as a functional target of TBX18. Our work suggests that TBX18 acts as a permissive factor...

... of the pulmonary mesothelium in dysregulated lung growth noted in the Wt1 knockout mouse model of CDH. Loss of WT1 leads to intrafetal effusions, altered lung growth, and branching defects prior to normal closure of the diaphragm. We found significant differences in key genes; however, when Wt1 null lungs were...

... apparatus WT1 Mouse The formation and growth of the mouse embryonic heart is a highly dynamic process that includes heart tube elongation and looping, chamber septation and growth. Early specified cardiac progenitors in the linear heart tube rapidly proliferate and differentiate, followed...

... a crucial role in embryonic heart development and adult heart repair; however, the molecular events underlying its maturation remain unknown. Wt1 , one of the main markers of the embryonic epicardium, is essential for epicardial development and function. Here, we analyse the transcriptomic profile...

...Nicholas D. Hastie ABSTRACT The study of genes mutated in human disease often leads to new insights into biology as well as disease mechanisms. One such gene is Wilms’ tumour 1 ( WT1 ), which plays multiple roles in development, tissue homeostasis and disease. In this Primer, I summarise how...

... gonads, but the mechanisms that control their differentiation during gonad development remain elusive. In this study, we found that deletion of Wt1 in the ovary after sex determination caused ectopic development of steroidogenic cells at the embryonic stage. Furthermore, differentiation of both Sertoli...

... been identified, including sonic hedgehog, bone morphogenetic protein 4 and multiple members of the Wnt/β-catenin signaling pathway. However, the regulation of these factors, including their induction, remains poorly understood. Here, we identify a crucial role for the Wilms' tumor 1 protein (WT1...

..., Pax2 or Wt1 gene function in the metanephric mesenchyme compromises the formation of the kidney. Moreover, it has been shown that the Hox11-Eya1-Pax2 complex activates the expression of Six2 and Gdnf in the metanephric mesenchyme to drive nephrogenesis. Here, we demonstrate that the orphan nuclear...

...Sunny Hartwig; Jacqueline Ho; Priyanka Pandey; Kenzie MacIsaac; Mary Taglienti; Michael Xiang; Gil Alterovitz; Marco Ramoni; Ernest Fraenkel; Jordan A. Kreidberg The Wilms' tumor suppressor 1 ( WT1 ) gene encodes a DNA- and RNA-binding protein that plays an essential role in nephron progenitor...

...-Catenin stabilization caused changes in Sertoli cell identity and misregulation of inter-Sertoli cell contacts. As Wt1 conditional knockout in Sertoli cells causes similar phenotypes to our stabilized β-catenin mutants, we then investigated the relationship of Wt1 and β-catenin in Sertoli cells and found...

... of these genes but notably reduces expression of Wt1,a transcription factor implicated in spleen morphogenesis and expressed in the mesothelium. These observations place Barx1 proximally within a Wt1 pathway of spleen development and reveal how a homeotic regulator employs different molecular mechanisms to mold...

... morphogenesis. The mesenchyme produces Gdnf, which stimulates branching, and the ureteric bud stimulates continued growth of the mesenchyme and differentiation of nephrons from the induced mesenchyme. Null mutation of the Wt1 gene eliminates outgrowth of the ureteric bud, but Gdnf has been identified...

..., D., Howells, N. and Englert,C. ( 1999 ). The Wilms tumor suppressor gene wt1 is required for development of the spleen. Curr. Biol. 9 , 837 -840. Hisa, T., Spence, S. E., Rachel, R. A., Fujita, M., Nakamura,T., Ward, J. M., Devor-Henneman, D. E., Saiki, Y., Kutsuna, H., Tessarollo, L. et...

... proliferation and differentiation of neuronal progenitor cells. Here, we report that the Wilms' tumour zinc-finger protein, Wt1, is required for normal formation of the olfactory epithelium. Wt1 was detected by immunohistochemistry in the developing olfactory epithelium of wild-type embryos between gestational...

... progenitor pool as well as for determining the proximal tubule and podocyte fates. * Authors for correspondence (e-mail: [email protected] and [email protected] ) 1 7 2003 © 2003. 2003 Metanephric culture Notch gamma-secretase DAPT Wt1 Mouse Notch proteins...

... differentiation is unaffected. In addition, pax2 . 1 expression in the lateral cells of the pronephric primordium is required to restrict the domains of Wilms’ tumor suppressor ( wt1 ) and vascular endothelial growth factor (VEGF) gene expression to medial podocyte progenitors. Ectopic podocyte-specific marker...

...Adrian W. Moore; Lesley McInnes; Jordan Kreidberg; Nicholas D. Hastie; Andreas Schedl The Wilms’ Tumour gene WT1 has important functions during development. Knock-out mice were shown to have defects in the urogenital system and to die at embryonic day E13.5, probably due to heart failure. Using...

... in a variety of kidney tumors. Yet, little is known about the signals regulating this transient expression pattern in the developing kidney. We have examined the spatial and temporal expression patterns of Pax-2 and the Wilms’ tumor suppresser protein WT1 with specific antibodies in developing mouse kidneys...