... ). Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon . Nat. Methods 11 , 529 - 534 . 10.1038/nmeth.2889 Gleason , J. E. and Eisenmann , D. M. ( 2010 ). Wnt signaling controls the stem cell-like asymmetric division of the epithelial...

... proteins. Although much focus has been on understanding intracellular Wnt signal transduction, the cellular machinery responsible for Wnt secretion became better understood only recently. After lipid modification by the acyl-transferase Porcupine, Wnt proteins bind their dedicated cargo protein Evi/Wntless...

...Paula Murphy; Chris Armit; Bill Hill; Shanmugasundaram Venkataraman; Patrick Frankel; Richard A. Baldock; Duncan R. Davidson ABSTRACT Wnt signalling controls patterning and differentiation across many tissues and organs of the developing embryo through temporally and spatially restricted expression...

... an important role in coordinating long-range extracellular levels of the hydrophobic morphogen Wnt/Wingless. Wnt secretion Wnt signalling Endosomal sorting Morphogen trafficking Deutsche Forschungsgemeinschaft 10.13039/501100001659 331351713 GR4810/2-1 Georg-August-Universität...

... of the terminal asymmetric divisions of neuronal progenitors. C. elegans Neuron Asymmetric division Polarity Wnt signalling Centre National de la Recherche Scientifique 10.13039/501100004794 Institut National de la Santé et de la Recherche Médicale 10.13039/501100001677...

... of Biologists Ltd 2019 Summary: This Review describes the trafficking mechanisms proposed to mediate the extracellular transport of Wnt proteins and discusses how they contribute to Wnt dispersal. Cytoneme Exosome Secretion Signal transduction Wnt signalling Wnt trafficking The Wnt...

... for the morphogenesis of the head and brain. The loss of function of the Wnt inhibitor Dkk1 results in elevated Wnt activity, loss of ectoderm lineage attributes from the anterior epiblast, and the posteriorisation of anterior germ layer tissue towards the mesendoderm. The modulation of Wnt signalling may therefore...

... , I. , Bugter , J. M. , Noordstra , I. , van der Kooij , J. , Low , T. Y. , Pimentel-Muiños , F. X. , Xanthakis , D. , et al. ( 2018 ). TMEM59 potentiates Wnt signaling by promoting signalosome formation . Proc. Natl. Acad. Sci. USA   115 , E3996 - E4005 . 10.1073/pnas...

... by cad and other pair-rule genes that are likely to operate in a circuit ( Small et al., 1991 , 1992 ; Fujioka et al., 1996 ; Choe et al., 2006 ; El-Sherif et al., 2014 ), probably downstream of Wnt signalling ( Oberhofer et al., 2014 ). Therefore, to better understand the regulation of segment...

... to target genomic loci serves as the hallmark of canonical Wnt signalling, mechanisms controlling stage- or tissue-specific transcriptional responses remain elusive. Here, a direct comparison of genome-wide occupancy of β-catenin with a stage-matched Wnt-regulated transcriptome reveals that only a subset...

... that accompanies the formation of the anterior mesendoderm, in part through regulation of Pcdh7 expression. LHX1 also regulates, in the anterior mesendoderm, the transcription of genes encoding negative regulators of WNT signalling, such as Dkk1 , Hesx1 , Cer1 and Gsc. Embryos carrying mutations in Pcdh7...

...Monica Nagendran; Prateek Arora; Payal Gori; Aditya Mulay; Shinjini Ray; Tressa Jacob; Mahendra Sonawane The patterning and morphogenesis of body appendages – such as limbs and fins – is orchestrated by the activities of several developmental pathways. Wnt signalling is essential for the induction...

... differentiation by promoting Wnt/β-catenin signalling in the developing forebrain. In addition, we show that Fezf2 promotes activation of Wnt/β-catenin signalling by repressing the expression of two negative regulators of Wnt signalling, namely lhx2 and lhx9 . Our findings suggest that Fezf2 plays an essential...

.../licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Wnt signalling Mesodermal Morphogenesis Neural Stem cells The nervous system comprises a cellular network that processes sensory and motor...

... EpiSCs are biased towards mesoderm and endoderm fates while retaining pluripotency. The acquisition of primitive streak characteristics by self-renewing EpiSCs is mediated by endogenous Wnt signalling. Elevation of Wnt activity promotes restriction towards primitive streak-associated lineages...

...Natalie Gibb; Danielle L. Lavery; Stefan Hoppler Wnt signalling is a key regulator of vertebrate heart development, yet it is unclear which specific Wnt signalling components are required to regulate which aspect of cardiogenesis. Previously, we identified Wnt6 as an endogenous Wnt ligand required...

... crypt axis with the highest levels at the crypt bottom. In addition, colorectal cancers show a heterogeneous subcellular pattern of β-catenin accumulation. However, it remains unclear whether different levels of Wnt signalling exert distinct roles in the colonic epithelium. Here, we investigated...

... by which the PLLP normally ends its migration, suggesting that suppression of Wnt signalling is required for termination of neuromast production in the tail. Our data support a model in which Lef1 sustains proliferation of leading zone progenitors, maintaining the primordium size and defining neuromast...

..., Parc Valrose, 06108 Nice cedex 2, France Competing interests statement The authors declare no competing financial interests. 27 5 2011 © 2011. Wnt signalling Liver Swim bladder Endoderm patterning Organogenesis Zebrafish A central focus...

...Anke Beermann; Romy Prühs; Rebekka Lutz; Reinhard Schröder Short germ embryos elongate their primary body axis by consecutively adding segments from a posteriorly located growth zone. Wnt signalling is required for axis elongation in short germ arthropods, including Tribolium castaneum...