... to an EGF patterning signal. C. elegans EGFR ERK Ras Dauer Vulva National Institute of General Medical Sciences http://dx.doi.org/10.13039/100000057 R35GM131746 T32GM008798 F31GM126741 Caenorhabditis elegans larvae display remarkable developmental plasticity...

... for the development of the vulva by promoting the localization of the sole Epidermal growth factor receptor (EGFR) ortholog LET-23 to the basolateral membrane of the vulva precursor cells where it can specify the vulval cell fate. To understand how the LIN-2/7/10 complex regulates receptor localization, we determined...

... metazoan development, but their role in controlling organ morphogenesis is less understood. Here, we show that the C. elegans hox gene lin-39 regulates epidermal morphogenesis via its novel target, the essential zinc finger protein VAB-23. During the development of the vulva, the egg-laying organ...

... is an important general regulator of Wnt activity, rather than a specific EGL-20 antagonist. As a system in which neighboring cells reproducibly adopt distinct fates, vulva induction has enabled us to study how CAM-1 affects the precision of Wnt distribution. The subtle effects we observed upon cam-1...

.... * These authors contributed equally to this work 15 6 2006 © 2006. 2006 † Author for correspondence (e-mail: [email protected] ) Caenorhabditis elegans Vulva Pumilio Translational control Signal transduction The spatial and temporal regulation of gene expression can...

... address: UCMP, Umea University, SE-901 87 Umea, Sweden 4 11 2003 © 2004. 2004 PP2A PR55 Raf PAR-1 Vulva C. elegans We examined sur-6(sv30) and sur-6(RNAi) embryos to determine the cause of lethality. Both types of embryos appeared similar and exhibited spindle...

... that involves activation of the heterotrimeric Gαq protein, EGL-30. EGL-30 acts with muscle-expressed EGL-19 L-type voltage-gated calcium channels to promote vulva development, and acts downstream or parallel to LET-60 (RAS). This pathway is not essential for vulval induction on standard Petri plates,but can...

... was correlated with a protruding vulva phenotype at the adult stage( Fig. 7E ). However, such animals were able to lay eggs normally. We also examined egl-17::GFP expression in lin-11 RNAi animals during the mid-L4 stage (∼4 hours after the heat shock treatment). Although the overall GFP pattern...

... to specificity of this class of mediator protein. * Author for correspondence (e-mail: [email protected] ) 4 10 2002 © 2003. 2003 EGF RAS LET-23 SOP-1 DPY-22 Mediator Vulva C. elegans Activation of signal transduction pathways by growth factors is frequently used...

...Kyunghee Koh; Sara M. Peyrot; Cricket G. Wood; Javier A. Wagmaister; Morris F. Maduro; David M. Eisenmann; Joel H. Rothman Development of the vulva in C. elegans is mediated by the combinatorial action of several convergent regulatory inputs, three of which,the Ras, Wnt and Rb-related pathways, act...

...Benno Jungblut; André Pires-daSilva; Ralf J. Sommer The invariant cell lineage of nematodes allows the formation of organ systems, like the egg-laying system, to be studied at a single cell level. The Caenorhabditis elegans egg-laying system is made up of the vulva, the mesodermal gonad...

... all detached from the cuticle, resulting in the formation of an abnormal vulva ( n =56) ( Fig. 3D ). The production of non-adhering cells is typical for the 1° sublineage, suggesting that P5.p and P7.p adopted a 1°-like or a 1°/2° hybrid cell fate ( Sternberg and Horvitz, 1989 ). These data suggest...

...Benno Jungblut; Ralf J. Sommer ABSTRACT Vulva development differs between Caenorhabditis elegans and Pristionchus pacificus in several ways. Seven of 12 ventral epidermal cells in P. pacificus die of apoptosis, whereas homologous cells in C. elegans fuse with the hypodermal syncytium. Vulva...

...Victor Ambros In Caenorhabditis elegans , the fates of the six multipotent vulva precursor cells (VPCs) are specified by extracellular signals. One VPC expresses the primary (1°) fate in response to a Ras-mediated inductive signal from the gonad. The two VPCs flanking the 1° cell each express...

...Ranjana Sharma-Kishore; John G. White; Eileen Southgate; Benjamin Podbilewicz The genes involved in the inductive interactions that specify cell fates in the vulva of Caenorhabditis elegans are known in some detail. However, little is known about the morphogenesis of this organ. Using a combination...

...Wendy Hanna-Rose; Min Han ABSTRACT In screens for mutants defective in vulval morphogenesis, multiple mutants were isolated in which the uterus and the vulva fail to make a proper connection. We describe five alleles that define the gene cog-2 , for co nnection of g onad defective. To form...

... of this pathway in the vulva precursor cells are two novel proteins, LIN-25 and SUR-2. In this report, we describe further studies of lin-25 . The results of a genetic mosaic analysis together with those of experiments in which lin-25 was expressed under the control of an heterologous promoter suggest...

...Ralf J. Sommer; Andreas Eizinger; Kwang-Zin Lee; Benno Jungblut; Anja Bubeck; Isabel Schlak ABSTRACT In the two nematode species Caenorhabditis elegans and Pristionchus pacificus the vulva equivalence group in the central body region is specified by the Hox gene lin-39. C. elegans lin-39 mutants...

... of Zoology, University of Wisconsin – Madison, Madison, WI 53706, USA ‡ Author for correspondence (e-mail: [email protected] ) 25 August 1998 10 July 1998 © 1998 by Company of Biologists 1998 β-catenin Vulva Caenorhabditis elegans Wnt Hox BAR-1 LET-60 Ras...

... to inductive signalling helps impose a bias on lateral signalling and contributes to the invariant pattern of VPC fates. * Author for correspondence (e-mail: [email protected]) © 1998 by Company of Biologists 1998 LIN-12 Notch Cell-cell interactions C. elegans Vulva EGF...