... of developmental quiescence, by a mechanism that desensitizes multipotential cells to an EGF patterning signal. C. elegans EGFR ERK Ras Dauer Vulva National Institute of General Medical Sciences http://dx.doi.org/10.13039/100000057 R35GM131746 T32GM008798 F31GM126741...

... for the development of the vulva by promoting the localization of the sole Epidermal growth factor receptor (EGFR) ortholog LET-23 to the basolateral membrane of the vulva precursor cells where it can specify the vulval cell fate. To understand how the LIN-2/7/10 complex regulates receptor localization, we determined...

... metazoan development, but their role in controlling organ morphogenesis is less understood. Here, we show that the C. elegans hox gene lin-39 regulates epidermal morphogenesis via its novel target, the essential zinc finger protein VAB-23. During the development of the vulva, the egg-laying organ...

.... These results demonstrate how ROR proteins help to refine the spatial pattern of Wnt activity in a complex multicellular environment. * Author for correspondence (e-mail: [email protected] ) 25 8 2007 © 2007. 2007 ROR CAM-1 Wnt Vulva Patterning CRD Organogenesis Morphogen...

... spermatocytes in hermaphrodites and males( Subramaniam and Seydoux,2003 ). † Author for correspondence (e-mail: [email protected] ) * These authors contributed equally to this work 15 6 2006 © 2006. 2006 Caenorhabditis elegans Vulva Pumilio Translational control...

... in the germline that could not be rescued as most C. elegans transgenes are transcriptionally silent in the germline( Kelly et al., 1997 ). The sterile phenotype of the rescued animals was similar to the sterile phenotype of wild-type larvae soaked in let-92 dsRNA. Vulva development was normal in the rescued...

... that involves activation of the heterotrimeric Gαq protein, EGL-30. EGL-30 acts with muscle-expressed EGL-19 L-type voltage-gated calcium channels to promote vulva development, and acts downstream or parallel to LET-60 (RAS). This pathway is not essential for vulval induction on standard Petri plates,but can...

... in this plane). Overall, vulval invagination was narrower along the anteroposterior axis compared with the wild type. This abnormal morphology was correlated with a protruding vulva phenotype at the adult stage( Fig. 7E ). However, such animals were able to lay eggs normally. We also examined egl-17::GFP...

.... Hermaphrodites heterozygous for let-23(sa62) have mostly wild-type vulvae, but occasionally some animals display ectopic vulval tissue( Table 1 ). sy622 and sy665 enhance the frequency of ectopic vulval fate specification in let-23(sa62) /+ animals ( Table 1 ), indicating that the mutations also affect...

...Kyunghee Koh; Sara M. Peyrot; Cricket G. Wood; Javier A. Wagmaister; Morris F. Maduro; David M. Eisenmann; Joel H. Rothman Development of the vulva in C. elegans is mediated by the combinatorial action of several convergent regulatory inputs, three of which,the Ras, Wnt and Rb-related pathways, act...

...Benno Jungblut; André Pires-daSilva; Ralf J. Sommer The invariant cell lineage of nematodes allows the formation of organ systems, like the egg-laying system, to be studied at a single cell level. The Caenorhabditis elegans egg-laying system is made up of the vulva, the mesodermal gonad...

...-mail: [email protected] ) 29 09 2000 14 11 2000 © 2000 by Company of Biologists 2000 Caenorhabditis elegans Mi-2 Development Vulva Ras Notch NuRD The Mi-2 protein, originally identified as an autoantigen of the human disease dermatomyositis ( Seelig et al., 1995...

...Benno Jungblut; Ralf J. Sommer ABSTRACT Vulva development differs between Caenorhabditis elegans and Pristionchus pacificus in several ways. Seven of 12 ventral epidermal cells in P. pacificus die of apoptosis, whereas homologous cells in C. elegans fuse with the hypodermal syncytium. Vulva...

...Victor Ambros In Caenorhabditis elegans , the fates of the six multipotent vulva precursor cells (VPCs) are specified by extracellular signals. One VPC expresses the primary (1°) fate in response to a Ras-mediated inductive signal from the gonad. The two VPCs flanking the 1° cell each express...

...Ranjana Sharma-Kishore; John G. White; Eileen Southgate; Benjamin Podbilewicz The genes involved in the inductive interactions that specify cell fates in the vulva of Caenorhabditis elegans are known in some detail. However, little is known about the morphogenesis of this organ. Using a combination...

...Wendy Hanna-Rose; Min Han ABSTRACT In screens for mutants defective in vulval morphogenesis, multiple mutants were isolated in which the uterus and the vulva fail to make a proper connection. We describe five alleles that define the gene cog-2 , for co nnection of g onad defective. To form...

... of this pathway in the vulva precursor cells are two novel proteins, LIN-25 and SUR-2. In this report, we describe further studies of lin-25 . The results of a genetic mosaic analysis together with those of experiments in which lin-25 was expressed under the control of an heterologous promoter suggest...

...Ralf J. Sommer; Andreas Eizinger; Kwang-Zin Lee; Benno Jungblut; Anja Bubeck; Isabel Schlak ABSTRACT In the two nematode species Caenorhabditis elegans and Pristionchus pacificus the vulva equivalence group in the central body region is specified by the Hox gene lin-39. C. elegans lin-39 mutants...

... . J. Cell Biol. 114 , 465 – 479 . 10.1083/jcb.114.3.465 Greenwald , I. ( 1997 ). Development of the vulva . In C. elegans II , (ed. D. L. Riddle , T. Blumenthal , B. J. Meyer and J. R. Priess ), pp. 519 – 41 . Cold Spring Harbor : Cold Spring Harbor...

... to inductive signalling helps impose a bias on lateral signalling and contributes to the invariant pattern of VPC fates. * Author for correspondence (e-mail: [email protected]) © 1998 by Company of Biologists 1998 LIN-12 Notch Cell-cell interactions C. elegans Vulva EGF...