... CRISPR/Cas9 genome editing in multiple species. CRISPR/Cas9 Oocyte delivery Vitellogenesis Gene editing Transgenesis National Institutes of Health R00HD099315 GM134577 Hibbitt Foundation National Institutes of Health The Eugene Bell Center...

...Shiming Zhu; Fangfang Liu; Huanchao Zeng; Na Li; Chonghua Ren; Yunlin Su; Shutang Zhou; Guirong Wang; Subba Reddy Palli; Jian Wang; Yiru Qin; Sheng Li ABSTRACT Vitellogenesis, including vitellogenin (Vg) production in the fat body and Vg uptake by maturing oocytes, is of great importance...

... acts via LCMT1-PP2A-FoxO to regulate Cdc2 and Orc5 expression, and to enhance ploidy of fat body cells in preparation for the large-scale Vg synthesis required for synchronous maturation of multiple eggs. A hallmark of female insect reproduction is vitellogenesis, a process by which Vg...

... by the oocyte. Drosophila Egg chamber Morphogenesis Muscular dystrophy Laminin Vitellogenesis When studying morphogenesis, it can often be difficult to tease apart the physical contributions that different cell types make to the form of a tissue or organ. The elongation of the Drosophila...

... of vitellogenesis. Here, we discovered that Drosophila Endophilin B (D-EndoB), a member of the endophilin family, is required for yolk endocytosis as it regulates membrane dynamics in developing egg chambers. Loss of D-EndoB leads to yolk content reduction, similar to that seen in yolkless mutants, and also causes...

... reveals maximum tubulin concentration in the Balbiani body and in a ring-shaped formation around the nucleus in young oocytes. The Balbiani body disintegrates in the course of vitellogenesis, granules formed from its periphery migrate into the cytoplasm and gradually fill the entire cytoplasm as radial...