... neural tube closure, genitourinary tract and hindgut defects. We show that, in Tmem132a mutant embryos, visceral endoderm fails to be excluded from the medial region of early hindgut, leading directly to the loss or malformation of cloaca-derived genitourinary and gastrointestinal structures...

... of the direction of cluster expansion. We carried out long-term and short-term lineage tracking. We also performed time-lapse imaging to characterize cellular behaviors using R26-PHA7-EGFP and R26R-EGFP . These images were subjected to quantitative image analyses. We found that the proximal visceral endoderm...

... interests. © 2015. Published by The Company of Biologists Ltd 2015 Summary: This Meeting Review summarises the work and advances presented at the recent EMBO Workshop on Embryonic-Extraembryonic Interfaces Extraembryonic tissues Placenta Visceral endoderm The legendary late Rosa...

... on existing alternatives. Such a device was microfabricated and its capacities tested by targeting the distal visceral endoderm (DVE), a migrating cell population essential for anterior-posterior axis establishment. Transfection was efficiently and reproducibly restricted to fewer than four visceral endoderm...

... be derived from the peri-implantation mouse embryo. Extra-embryonic endoderm stem (XEN) cells resemble the primitive endoderm of the blastocyst, which normally gives rise to the parietal and the visceral endoderm in vivo, but XEN cells do not contribute efficiently to the visceral endoderm in chimeric...

... fails to increase significantly in size after E7.0, indicating that middle primitive streak assembly is mechanistically tied to the growth of the embryo during gastrulation. Amn, a novel transmembrane protein, is expressed exclusively in an extra-embryonic tissue, visceral endoderm (VE), during...

... suggest patterning roles for anterior visceral endoderm (AVE) and ADE. Based on similarity of gene expression patterns, fate and a dual role in`protecting' the prospective forebrain from caudalising influences of the organiser, the chick hypoblast has been suggested to be the homologue of the mouse...

... of the definitive endoderm in the foregut. In addition, the mid- and hindgut tissues fail to expand. These are accompanied by the replacement of the definitive endoderm in the lateral region of the entire length of the embryonic gut by cells that resemble the visceral endoderm. In the chimeras, although Sox17 -null...

... of the embryo. At the onset of gastrulation, VEGFA is primarily expressed in the yolk sac visceral endoderm and in the yolk sac mesothelium. We report the generation and analysis of a Vegf hypomorphic allele, Vegf lo . Animals heterozygous for the targeted mutation are viable. Homozygous embryos, however, die...

... neural plate, but from 8.5 days post coitum they developed severe forebrain and midbrain abnormalities. OTX2 protein levels in Otx2 λ mutants were heavily reduced in the epiblast, axial mesendoderm and anterior neuroectoderm but not in the visceral endoderm. At the molecular level, we found out...

...Aitana Perea-Gomez; Kirstie A. Lawson; Muriel Rhinn; Lise Zakin; Philippe Brûlet; Sylvie Mazan; Siew-Lan Ang ABSTRACT Genetic and embryological experiments have demonstrated an essential role for the visceral endoderm in the formation of the forebrain; however, the precise molecular and cellular...

...Juan Pedro Martinez Barbera; Melanie Clements; Paul Thomas; Tristan Rodriguez; Denise Meloy; Dimitris Kioussis; Rosa S. P. Beddington ABSTRACT The homeobox gene Hex is expressed in the anterior visceral endoderm (AVE) and rostral definitive endoderm of early mouse embryos. Later, Hex transcripts...

... provide cues that predict the axes of the postimplantation embryo, we have used the strategy of injecting mRNA encoding an enduring marker to trace the progeny of inner cell mass cells into the postimplantation visceral endoderm. This tissue, although it has an extraembryonic fate, plays a role in axis...

...William Shawlot; Maki Wakamiya; Kin Ming Kwan; Artur Kania; Thomas M. Jessell; Richard R. Behringer Lim1 is a homeobox gene expressed in the extraembryonic anterior visceral endoderm and in primitive streak-derived tissues of early mouse embryos. Mice homozygous for a targeted mutation of Lim1 lack...

...Elena Barbacci; Michaël Reber; Marie-Odile Ott; Christelle Breillat; François Huetz; Silvia Cereghini Genetic and molecular evidence indicates that visceral endoderm, an extraembryonic cell lineage, is required for gastrulation, early anterior neural patterning, cell death and specification...

.... In addition to this late requirement, vHNF1 may play earlier roles during development, as it is first expressed in the visceral endoderm at the onset of gastrulation. In order to shed light on its function during embryogenesis, we have inactivated the murine gene by homologous recombination. The homozygous...

...Aitana Perea-Gómez; William Shawlot; Hiroshi Sasaki; Richard R. Behringer; Siew-Lan Ang Recent embryological and genetic experiments have suggested that the anterior visceral endoderm and the anterior primitive streak of the early mouse gastrula function as head- and trunk-organising centers...

...Yoko Suda; Jun Nakabayashi; Isao Matsuo; Shinichi Aizawa Mice have two Otx genes, Otx1 and Otx2 . Prior to gastrulation, Otx2 is expressed in the epiblast and visceral endoderm. As the primitive streak forms, Otx2 expression is restricted to the anterior parts of all three germ layers. Otx1...

... ). Of these genes, only Cr1 is expressed in teratocarcinoma F9 cells (our unpublished data) and embryo stem (ES) cells. Gene targeting Homologous recombination Mesoderm Visceral endoderm Neuroepithelium Gastrulation Cardiac myosin Mouse © 1999 by Company of Biologists 1999 07 January...

...Electra Coucouvanis; Gail R. Martin ABSTRACT At E4.0 the inner cell mass of the mouse blastocyst consists of a core of embryonic ectoderm cells surrounded by an outer layer of primitive (extraembryonic) endoderm, which subsequently gives rise to both visceral endoderm and parietal endoderm. Shortly...