...Sierra Duca; Yu Xia; Laila Abd Elmagid; Isaac Bakis; Miaoyan Qiu; Yingxi Cao; Ylan Guo; James V. Eichenbaum; Megan L. McCain; Junsu Kang; Michael R. M. Harrison; Jingli Cao ABSTRACT Vascular endothelial growth factor C (Vegfc) is crucial for lymphatic and blood vessel development, yet its cellular...

... endothelial-to-hematopoietic transition. The factors and signals that control HSPC fate decisions from the hemogenic endothelium are not fully understood. We found that Vegfc has a role in HSPC emergence from the zebrafish DA. Using time-lapse live imaging, we show that some HSPCs in the DA of vegfc loss...

... the formation of intersegmental arteries by primary angiogenesis and Vegfc is essential for secondary angiogenesis, giving rise to veins and lymphatics. Vegfd has been largely thought of as dispensable for vascular development in vertebrates. Here, we generated a zebrafish vegfd mutant by genome editing. vegfd...

...Masahiro Shin; Ira Male; Timothy J. Beane; Jacques A. Villefranc; Fatma O. Kok; Lihua J. Zhu; Nathan D. Lawson Vascular endothelial growth factor C (Vegfc) activates its receptor, Flt4, to induce lymphatic development. However, the signals that act downstream of Flt4 in this context in vivo remain...

... vessels. Vegfr3 signalling, through its ligand Vegfc and the extracellular protein Ccbe1, is essential for the sprouting of LECs to form the trunk lymphatic network. In this study we determined whether Vegfr3, Vegfc and Ccbe1 are also required for development of the facial and intestinal lymphatic...

... and found that Vegfc in the DA and Vegfr3 in the motoneurons were essential for the axon growth. Forced expression of either Vegfc in arteries or Vegfr3 in motoneurons resulted in enhanced axon growth of motoneurons over the DA. Both vegfr3 morphants and vegfc morphants lost the alignment of motoneuron...

.... , Schulte-Merker S. ( 2009b ). Vegfc/Flt4 signalling is suppressed by Dll4 in developing zebrafish intersegmental arteries . Development 136 , 4001 – 4009 . Iruela-Arispe M. L. , Davis G. E. ( 2009 ). Cellular and molecular mechanisms of vascular lumen formation . Dev. Cell 16...

...Jacques A. Villefranc; Stefania Nicoli; Katie Bentley; Michael Jeltsch; Georgia Zarkada; John C. Moore; Holger Gerhardt; Kari Alitalo; Nathan D. Lawson Vascular endothelial growth factor C (Vegfc) is a secreted protein that guides lymphatic development in vertebrate embryos. However, its role...

...Aniket V. Gore; Matthew R. Swift; Young R. Cha; Brigid Lo; Mary C. McKinney; Wenling Li; Daniel Castranova; Andrew Davis; Yoh-suke Mukouyama; Brant M. Weinstein Here, we show that a novel Rspo1-Wnt-Vegfc-Vegfr3 signaling pathway plays an essential role in developmental angiogenesis. A mutation in R...

..., yet the dll4 loss-of-function arterial hyperbranching phenotype was rescued, in flt4 signalling mutants. Furthermore, we found that the Flt4 ligand Vegfc drives arterial hyperbranching in the absence of dll4. Upon knockdown of dll4 , intersegmental arteries were sensitised to increased vegfc levels...