... cell mass and the trophectoderm (TE). In mice, TEAD4 is a master regulator of TE commitment, as it regulates the expression of other TE-specific genes and its ablation prevents blastocyst formation, but its role in other mammals remains unclear. Herein, we have observed that TEAD4 ablation in two...

... of development, human embryos form a blastocyst composed of an inner cell mass and trophectoderm (TE) cells, the latter of which are progenitors of placental trophoblast. Here, we investigated the expression of transcripts in the human TE from early to late blastocyst stages. We identified enrichment...

...Anish Dattani; Tao Huang; Corin Liddle; Austin Smith; Ge Guo ABSTRACT Propagation of human naïve pluripotent stem cells (nPSCs) relies on the inhibition of MEK/ERK signalling. However, MEK/ERK inhibition also promotes differentiation into trophectoderm (TE). Therefore, robust self-renewal requires...

... for trophectodermal Tat-Cre/loxP recombination. We used the natural permeability barrier in mouse blastocysts in combination with off-the-shelf Tat-Cre recombinase to achieve editing of conditional alleles in the trophoblast lineage. This direct approach enables gene function analysis during implantation...

... NANOG expression in human epiblast cells, consistent with a requirement for this pathway in pluripotency. Although the key trophectoderm factors Id2 , Elf5 and Eomes are exclusively localized to this lineage in the mouse, the human orthologues are either absent or expressed in alternative lineages...

... how the chromatin remodelling protein CHD4 facilitates the first lineage decision in mammalian embryogenesis. Embryos lacking CHD4 can form a morphologically normal early blastocyst, but are unable to successfully complete the first lineage decision and form functional trophectoderm (TE...

...Tomoko Watanabe; John S. Biggins; Neeta Bala Tannan; Shankar Srinivas The formation of trophectoderm (TE) and pluripotent inner cell mass (ICM) is one of the earliest events during mammalian embryogenesis. It is believed that the orientation of division of polarised blastomeres in the 8- and 16...

... outer trophectoderm (TE) and internal primitive endoderm (PE) in the blastocyst and subsequently give rise to chorio-allantoic and visceral yolk sac placentae, respectively. We have shown maternal low protein diet exclusively during mouse preimplantation development (Emb-LPD) is sufficient to cause...

...Kotaro J. Kaneko; Melvin L. DePamphilis It has been suggested that during mouse preimplantation development, the zygotically expressed transcription factor TEAD4 is essential for specification of the trophectoderm lineage required for producing a blastocyst. Here we show that blastocysts can form...

[email protected] ) Competing interests statement The authors declare no competing financial interests. 14 8 2012 © 2012. 2012 Blastocyst Maternal-effect gene Trophectoderm Caudal In animals, early embryonic development is regulated by maternal genes, which are transcribed...

... and the trophectoderm. Shortly after implantation, the anterior-posterior axis is firmly established. Recent studies have provided a better understanding of how the earliest cell fate decisions are regulated in the pre-implantation embryo, and how and when the body axes are established in the pregastrulation embryo...

...Guangming Wu; Luca Gentile; Takuya Fuchikami; Julien Sutter; Katherina Psathaki; Telma C. Esteves; Marcos J. Araúzo-Bravo; Claudia Ortmeier; Gaby Verberk; Kuniya Abe; Hans R. Schöler The separation of the first two lineages – trophectoderm (TE) and inner cell mass (ICM) – is a crucial event...

...-implantation embryo. Here, we show that Klf5 is required for the formation of the trophectoderm (TE) and the inner cell mass (ICM), and for repressing primitive endoderm (PE) development. Although cell polarity appeared normal, Klf5 mutant embryos arrested at the blastocyst stage and failed to hatch due...

...Robert Odell Stephenson; Yojiro Yamanaka; Janet Rossant The first two cell lineages in the mouse, the surface trophectoderm (TE) and inner cell mass (ICM), are morphologically distinguishable by E3.5, with the outer TE forming a polarized epithelial layer enclosing the apolar ICM. We show here...

... at preimplantation stages ( Fig. 4A-E ). Gata3 protein was detectable within the nuclei of the trophectoderm at the blastocyst stage, where it colocalized with Cdx2 ( Fig. 4D ) ( n =10). In fact, Gata3 colocalized with Cdx2 at earlier preimplantation stages as well ( Fig. 4A-C ) ( n =31 embryos, 8- to 32-cell stages...

... to facilitate or to suppress cell-cycle progression. In stark contrast to the milder phenotypes that result from inactivation of E2Fs, we report that loss of Dp1 leads to death in utero because of the failure of extra-embryonic development. Loss of Dp1 compromises the trophectoderm-derived tissues...

...Bhavwanti Sheth; Breda Moran; James M. Anderson; Tom P. Fleming ABSTRACT The mouse blastocyst forms during the 32-cell stage with the emergence of the blastocoelic cavity. This developmental transition is dependent upon the differentiation and transport function of the trophectoderm epithelium...

...Bhavwanti Sheth; Ira Fesenko; Jane E. Collins; Breda Moran; Arthur E. Wild; James M. Anderson; Tom P. Fleming ABSTRACT The mouse preimplantation embryo has been used to investigate the de novo synthesis of tight junctions during trophectoderm epithelial differentiation. We have shown previously...

... and laminin. Collagen type IV, gelatin, vitronectin or entactin supported little or no endodermal outgrowth. Trophectoderm (TE) cells have been implied to be important in PE induction in vivo. We found that recombination of ICMs in culture with TE cells, or with medium conditioned by TE cells, greatly...

.... Previously, we have shown that desmocollin protein synthesis is undetectable in eggs and cleavage stages but initiates at the early blastocyst stage when desmocollin localises at, and appears to regulate assembly of, nascent desmosomes that form in the trophectoderm but not in the inner cell mass (Fleming, T...