... highlights promising clinical results and discusses challenges associated with clinical translation. Human pluripotent stem cells Cell therapy Transplantation Clinical trial ATMP Horizon 2020 Framework Programme http://dx.doi.org/10.13039/100010661 874758 Novo Nordisk...

... reduced the number of B1a cells, MZBs, ILC2s and Tregs, both in situ and upon transplantation, indicating an intrinsic and essential role for IL7Rα. Surprisingly, reciprocal transplants of wild-type HSCs showed that an IL7Rα −/− environment selectively impaired reconstitution of TLCs when compared...

... demonstrated by the induction of the striatal transcription factors CTIP2, GSX2 and FOXP2. Crucially, these human LGE progenitors readily differentiate into postmitotic neurons expressing the striatal projection neuron signature marker DARPP32, both in culture and following transplantation in the adult...

...., Learish, R. D., Karram, K.,Choudhary, K., Wiestler, O. D., Duncan, I. D. and McKay, R. D. (1999). Embryonic stem cell-derived glial precursors: a source of myelinating transplants. Science 285 , 754 -756. Cai, J., Qi, Y., Hu, X., Tan, M., Liu, Z., Zhang, J., Li, Q.,Sander, M. and Qiu, M. (2005...

... and generated offspring following spermatogonial transplantation into the testes of infertile recipient mice, indicating that they are functionally normal. These results demonstrate that activation of the PI3K-Akt pathway plays a central role in the self-renewal division of spermatogonial stem cells...

... is sufficient to induce a fully functional testicular soma capable of supporting complete spermatogenesis in the XX body. Here, we demonstrate that the testicular somatic environment of XX/Sry males is defective in supporting the later phases of spermatogenesis. Spermatogonial transplantation analyses using XX...

... a single progenitor to theoretically form sufficient cells for∼4×10 7 adult brains. AHNPs longitudinally maintain the ability to generate both glial and neuronal cell types in vivo and in vitro,and are amenable to genetic modification and transplantation. These findings suggest an unprecedented degree...

..., and the maturation process, from epiblast cells through PGCs to postnatal spermatogonia, can occur in the postnatal testicular environment. Primordial germ cell transplantation techniques will also provide a novel tool to assess the developmental potential of PGCs, such as those manipulated in vitro or recovered...

... migration in splotch mutant embryos requires the genetic defect in both neural crest cells and their migratory environment. * Author for correspondence (e-mail: [email protected]) 24 3 2004 © 2004. 2004 Cardiac neural crest Migration splotch Labelling Transplantation Embryo...

...-cell transplantation. Proc. Natl. Acad. Sci. USA 91 , 11298 -11302. Bunting, K. (2002). ABC Transporters as Phenotypic Markers and Functional Regulators of Stem Cells. Stem Cells 20 , 11 -20. Chiarini-Garcia, H. and Russell, L. D. (2001). High-resolution light microscopic...

...Anne Holz; Barbara Bossinger; Thomas Strasser; Wilfried Janning; Robert Klapper As in many other organisms, the blood of Drosophila consists of several types of hemocytes, which originate from the mesoderm. By lineage analyses of transplanted cells, we specified two separate anlagen that give rise...

.... embryos were orthotopically transplanted back to the same site and heterotopically transplanted into r2 and r3 of isochronic embryos. Small fragments of mesoderm or ectoderm were isolated from adjacent to r3 and transplanted next to r4 in isochronic embryos. Grafted embryos were analysed...

...Robert Klapper; Sandra Heuser; Thomas Strasser; Wilfried Janning In order to reveal syncytia within the visceral musculature of Drosophila melanogaster , we have combined the GAL4/UAS system with the single-cell transplantation technique. After transplantation of single cells from UAS-GFP donor...

... from an early stage (Stainier,D. Y. R., Weinstein, B. M., Detrich, H. W. R., Zon, L. I. and Fishman, M. C. (1995) Development 121, 3141-3150). cloche mutants lack endocardium, as well as head and trunk endothelium, and nearly all blood cells. Cell transplantation studies have revealed...

[email protected]) 27 10 1999 27 11 1999 © 1999 by Company of Biologists 1999 Regeneration Blastema formation Dorsoventral interaction Planarian Chimera Transplantation The planarian is remarkable in its ability to regenerate. Even a small fragment cut from the body...

... of the mature placenta, study of differentiation of two of its component structures, the allantois and the chorion, has so far largely been neglected. Allantois Endothelial cell Erythropoiesis flk-1 In vitro Mesothelium Mouse Placenta Transplantation Vasculogenesis Yolk sac © 1998...

...Edith C. Kordon; Gilbert H. Smith ABSTRACT Any epithelial portion of a normal mouse mammary gland can reproduce an entire functional gland when transplanted into an epithelium-free mammary fat pad. Mouse mammary hyperplasias and tumors are clonal dominant populations and probably represent...

... ; McKay et al., 1994). In the latter interpretation, MafB/Kr would downregulate Hoxb-4 and Hoxd-4 expression in r5/6 under normal conditions and the absence of Kreisler expression in Kr/Kr mutant would allow the anterior extension of paralogue group four Hox genes. Our transplantation...

... by transplantation of midline cells as well as by homotopic expression of single-minded , the master gene for midline development. Furthermore, ectopic midline cells are able to induce enhanced expression of some lateral CNS cell markers. We thus conclude that the CNS midline plays an important role...

..., which is expressed in the ventricular zone, in cortical development. Embryos homozygous for a mutation of Pax-6 (Small eye; Sey) had abnormalities suggesting defective migration of late-born cortical precursors. When late-born Sey/Sey precursors were transplanted into wild-type embryonic rat cortex...