...) ( Ramalho et al., 2020 ). However, both erm-1[T544A] mRNA and wild-type erm-1 mRNA failed to concentrate at the apical membrane. Together, this suggests that in polarized cells the T544-dependent movement of ERM-1 protein from the basolateral to apical membranes occurs post-translationally, leaving...

..., and revealed four modes of translational selectivity: (1) selective translation of non-abundant mRNAs; (2) active, but modest translation of a selection of highly expressed mRNAs; (3) translationally suppressed abundant to moderately abundant mRNAs; and (4) mRNAs associated specifically with monosomes...

... on translationally repressed mRNAs with short polyA-tails ( Kim and Richter, 2007 ). This suggests that mRNAs bound by the Zar1l RNP could potentially be controlled by regulated polyadenylation during later developmental states. In addition to the aforementioned proteins, many of the identified Zar1l interaction...

...Mansour Aboelenain; Karen Schindler ABSTRACT Mammalian oocytes are transcriptionally quiescent, and meiosis and early embryonic divisions rely on translation of stored maternal mRNAs. Activation of these mRNAs is mediated by polyadenylation. Cytoplasmic polyadenylation binding element 1 (CPEB1...

... of the expression domain in nc14 ( Fig. 7 A). SunTag-hb mRNAs are still present in the anterior in nc14, but ∼95% are not being translated, suggesting that they are being translationally repressed. In addition to the highest number of SunTag-hb mRNA translation sites being detected at nc13, analysis...

... Translation Parkinson's disease (PD) is a debilitating neurodegenerative movement disorder resulting from progressive loss of dopaminergic (DA) neurons in the midbrain. The relatively focal degeneration makes it a good candidate for cell-based therapies. Since the first clinical trials using fetal...

...Nathalie Oulhen; S. Zachary Swartz; Jessica Laird; Alexandra Mascaro; Gary M. Wessel Stem cells in animals often exhibit a slow cell cycle and/or low transcriptional activity referred to as quiescence. Here, we report that the translational activity in the primordial germ cells (PGCs) of the sea...

... Human Organoid Pluripotency Translation Inspired by the success of the first meeting two years ago, Development and The Company of Biologists held the second ‘From Stem Cells to Human Development’ meeting in September 2016 in historic Southbridge (Massachusetts, USA). From the first human...

... is essential for: (1) general mRNA translation during embryogenesis, (2) developmental re-programming upon manipulations to the embryo and (3) larval wound healing. We also learned that Vasa interacted with mRNAs in the perinuclear area and at the spindle in an Importin-dependent manner during cell cycle...

... of which can lead to developmental defects and disease. Here, we examine the spectrum of molecular mechanisms controlling Hox gene expression in model vertebrates and invertebrates and find that a diverse range of mechanisms, including nuclear dynamics, RNA processing, microRNA and translational regulation...

... differentiation and partners with the serine/threonine kinase Fused to reduce levels of the Dpp receptor Tkv in cystoblasts ( Casanueva and Ferguson, 2004 ; Xia et al., 2010 ). The TRIM-NHL domain protein Brat also functions in cystoblasts, serving to translationally repress Mad expression ( Harris et al., 2011...

... as a positive regulator of target gene expression ( Kaye et al., 2009 ). GLD-1 is also a translational repressor ( Jan et al., 1999 ) with many target mRNAs ( Wright et al., 2010 ) and roles in both sex determination and meiotic progression ( Francis et al., 1995b ). gld-1 is itself both positively...

...Solenn Patalano; Marija Mihailovich; Yaiza Belacortu; Nuria Paricio; Fátima Gebauer Dosage compensation in Drosophila melanogaster involves the assembly of the MSL-2-containing dosage compensation complex (DCC) on the single X chromosome of male flies. Translational repression of msl-2 mRNA blocks...

...Charles R. Tessier; Kendal Broadie Fragile X Syndrome (FraX) is a broad-spectrum neurological disorder with symptoms ranging from hyperexcitability to mental retardation and autism. Loss of the fragile X mental retardation 1 ( fmr1 ) gene product,the mRNA-binding translational regulator FMRP...

... translation. The molecular basis of how the translation of mRNAs encoding guidance proteins could be closely tied to the pace of cytoskeletal assembly is poorly understood. Previous studies have shown that the F-actin-microtubule crosslinker Short stop (Shot) is required for motor and sensory axon extension...

... to a meiotic checkpoint, activated by a delay in DNA double-strand break (DSB) repair during oogenesis, Vas is post-translationally modified, and this corresponds to a downregulation in Grk protein accumulation( Ghabrial and Schüpbach,1999 ). In previous work, we identified a translation factor dIF2, now...

... of Hoxb4 expression is restricted by regulating transcript stability in the paraxial mesoderm and by selective translation and/or degradation of protein in the neural tube. Moreover, the absence of Hoxb4 3′-untranslated sequences from transgene transcripts leads to inappropriate expression of some Hoxb4...

...Veronica A. Marin; Thomas C. Evans In C. elegans , the Notch receptor GLP-1 is localized within the germline and early embryo by translational control of glp-1 mRNA. RNA elements in the glp-1 3′untranslated region (3′ UTR) are necessary for repression of glp-1 translation in germ cells...

... expressed at high levels at the posterior pole,where it is translated ( Ephrussi et al.,1991 ; Kim-Ha et al.,1991 ). Prior to its localization, osk mRNA is translationally repressed through the binding of Bruno (Bru) repressor protein to discrete elements(Bruno Response Elements or BRE) present...

... ( Bouvet and Wolffe, 1994 ). MSY2 and MSY4 exist in an mRNP complex in mouse spermatocytes, and both proteins bind sequences within the 3′ UTR of Prm1 , a translationally repressed mRNA ( Davies et al., 2000 ). Based on the involvement of vertebrate Y-box proteins in translational regulation of stored...