...Peter Koopman; Andrew Sinclair; Robin Lovell-Badge ABSTRACT On Thursday 9 May 1991, the world awoke to front-page news of a breakthrough in biological research. From Washington to Wollongong, newspapers, radio and TV were abuzz with the story of a transgenic mouse in London called Randy. Why...

... development RhoA ROCK Epithelial-mesenchymal interaction Transgenic mouse © 2008. 2008 12 8 2008 * These authors contributed equally to this study † Author for correspondence (e-mail: [email protected] ) ...

... in a male germ cell deficiency, irrespective of the co-expression of Nanos3 in these cells. These results indicate that these two Nanos proteins have distinct functions, which depend on the time and place of their expression. To further elucidate this, we have generated transgenic mouse lines that express...

...), diluted 1/200 in PBST/1.0% BSA, was incubated for 1 hour at room temperature. Sections were counterstained with DAPI and cover slipped using Vectashield (Vector Labs). lacZ staining of frozen sections was performed as described in Ishii et al. ( Ishii et al.,2003 ). Transgenic mouse embryos...

... regulators controlling outflow tract and right ventricle development. * Author for correspondence (e-mail: [email protected] ) 6 5 2004 ©2004. 2004 Mef2c transgenic mouse cardiac development transcription secondary heart field anterior heart field ISL1 (ISL-1) GATA4...

[email protected] ) 10 11 1999 12 01 2000 © 2000 by Company of Biologists 2000 TGFα EGF EGF Receptor Corneal endothelial cell Anterior segment dysgenesis Eye Transgenic mouse In mouse, the development of the anterior segment of the eye begins at about embryonic day 11 (E11...

... of reduced osteoclast activity was found in transgenic mouse skulls, which had less cavitation and fewer mature osteoclasts relative to skulls of wild-type mice. These bone remodeling defects resulted in altered biomechanical properties. The femurs of transgenic mice were tougher, and their vertebral bodies...

... embryonic cleft palate. * Author for correspondence (e-mail: [email protected] ) 1 6 1999 5 8 1999 © 1999 by Company of Biologists 1999 Palatal development Cleft palate Medial edge epithelial cells Transforming growth factor-β3 (TGF-β3) Transgenic mouse Filopodia...

... MEF2 mRNA expression and MEF2 transcriptional activity in nonmuscle cell types of embryos and adults also supports the notion that post-transcriptional mechanisms regulate the expression of MEF2 proteins. MEF2 Transcriptional activity Transgenic mouse MEF2C transcripts are also expressed...

... in mouse embryos. To elucidate the cis control mechanisms that regulate MyoD transcription, we have mutagenized the entire core enhancer using linker-scanner mutagenesis, and have tested the transcriptional activity of enhancer mutants using lacZ reporter gene expression in transgenic mouse embryos...

... Neural crest Hoxa2 AP-2 Transgenic mouse Gene regulation Fig. 7. The AP-2 site from the Hoxa2 enhancer functions as an AP-2 -responsive element in cultured cells. (A) Relative increase in transcription of reporter plasmids (indicated in B), co-transfected with the three murine...

[email protected] ) 22 05 1998 © 1998 by Company of Biologists 1998 Kit steel factor Melanocyte development Melanoblast Transgenic mouse c-Kit The c-Kit receptor tyrosine kinase of the mouse and its ligand, steel factor (SLF), are encoded by the dominant white spotting ( Kit...

... 31 10 1996 © 1997 by Company of Biologists 1997 Pax3 P19 embryonal carcinoma transgenic mouse DNA sequence elements enhancer transcriptional regulatory elements dorsoventral patterning Pax3 is one of nine members of a family of transcription factors that have...

... Institute of Biomolecular Medicine, 540 First Avenue, New York, NY 10016, USA 30 05 1996 © 1996 by Company of Biologists 1996 sonic hedgehog cAMP-dependent protein kinase A CNS patterning transgenic mouse The notochord has been known for some time to act as the source...

... differentiation. mammary gland galactosyltransferase morphogenesis transgenic mouse mouse The authors thank D. Mansfield and S. Khounlo for excellent technical assistance, and A. Youakim, D. Miller and members of the laboratory for critical reading of the manuscript. This work was supported...

.... * Author for correspondence 18 02 1994 © 1995 by Company of Biologists 1995 in ovo transplantation mouse somite graft desmin transgenic mouse chimera In vertebrates, skeletal muscle is derived from somites that develop by segmentation of the paraxial mesoderm. In the mouse...

... us to deliver genes specifically into this cell type in vivo to test specifically molecules that have been implicated in cardiovascular development. Furthermore, we have established embryonic stem (ES) cells from the blastocysts of the transgenic mouse that carry the 1.2 kb promoter-LacZ reporter...

... and reached a peak of 21.32% at 12 hours (a 1.4-fold increase). Clearly, mitotic activity of the duct cells was extremely high in this transgenic mouse strain. In contrast, the islet structures contained only 1.89% BrdUpositive cells after the first 6 hours of incubation. However, the number of labeled islet...

... transgenic mouse cell signalling cell proliferation pattern formation The vertebrate central nervous system (CNS) arises as a flattened plate of neuroepithelium, which rounds up to form a hollow tube (see Schoenwolf and Smith, 1990 , for review). Initially the neural tube consists of a single...

... Washington Street, Box 218, Boston, MA 02111, USA 28 10 1993 © 1994 by Company of Biologists 1994 peptide YY islet cell lineage transgenic mouse pancreas The expression of the transgene in other islet cell types does not reflect inappropriate expression since the endogenous...