...; Chaverra-Rodriguez et al., 2018 ), they exploit non-conserved proteins, and are therefore limited in their cross-species utility. Thus, our results set the stage for broader use of vitellogenin-based transgenesis in diverse emerging model systems. We identify VitelloTag B as a potential cross-reactive...

...; Nicolas Rohner; C. Ben Lovely; Kristen M. Kwan; Alexa Burger; Christian Mosimann ABSTRACT Transgenesis is an essential technique for any genetic model. Tol2-based transgenesis paired with Gateway-compatible vector collections has transformed zebrafish transgenesis with an accessible modular system. Here...

...Jennifer Anne Roberts; Irene Miguel-Escalada; Katherine Joan Slovik; Kathleen Theodora Walsh; Yavor Hadzhiev; Remo Sanges; Elia Stupka; Elizabeth Kate Marsh; Jorune Balciuniene; Darius Balciunas; Ferenc Müller Zebrafish transgenesis is increasingly popular owing to the optical transparency...

... of biological systems, the ability to move transgenesis experiments efficiently between organisms becomes essential. DNA constructions used in transgenesis usually contain four elements, including sequences that facilitate transgene genome integration, a selectable marker and promoter elements driving a coding...

... statement The authors declare no competing financial interests. 5 9 2011 © 2011. Transgenesis Er71 ( Etv2 ) knockout Mouse Transcriptional networks, signaling cascades, microRNAs and other factors coordinately regulate stem and progenitor cell populations to give rise...

... recapitulate endogenous expression patterns and levels is often difficult and using large genomic DNA fragments, such as bacterial artificial chromosomes (BACs), makes the process of transgenesis less reliable. Here we provide a detailed protocol that allows for BAC selection and subsequent rapid modification...

... the results and commented on the manuscript. Competing interests statement The authors declare no competing financial interests. 11 4 2011 © 2011. 2011 Parhyale Gene trapping ϕC31 integrase Regeneration Transgenesis iTRAC Although established genetic models offer...

... transgenesis: 25 ng/μl of each transgenesis vector were combined with 2.5 ng/μl Tol2 mRNA as described ( Kwan et al., 2007 ) and injected into one-cell-stage eggs derived from AB or Tu wild-type crosses, and the injected animals grown up to adulthood. Individual F0 founders were outcrossed to TL...

... Heart Xenopus laevis Xenopus tropicalis SMAD1 SMAD4 T-box BMP Zebrafish Transgenesis A series of clinical studies has provided direct evidence of a role for T-box genes in heart development and human disease, as mutations in at least three T-box genes, TBX1, TBX5...

... for organogenesis, knowledge of the regulation of Wt1 expression is scarce. Here, we have used transgenesis in zebrafish harboring two wt1 genes, wt1a and wt1b , in order to define regulatory elements that drive wt1 expression in the kidney. Stable transgenic lines with approximately 30 kb of the upstream genomic...

... Nkx2-5 Smooth muscle Transgenesis Knowledge of the regulatory mechanisms underlying cardiac development is essential for understanding the molecular basis of congenital heart defects. During embryonic heart development, differentiation of cardiac muscle is regulated by interactions between...

..., the potentialities and fate of MH precursors in these conditions have not been directly determined. To follow the dynamics of MH maintenance in vivo, we used artificial chromosome transgenesis in zebrafish to construct lines where egfp transcription is driven by the complete set of regulatory elements of her5...

... ). Modification of bacterial artificial chromosomes through chi-stimulated homologous recombination and its application in zebrafish transgenesis. Proc. Natl. Acad. Sci. USA 95 , 5121 -5126. Joyner, A. L. and Martin, G. R. ( 1987 ). En-1 and En-2, two mouse genes with sequence homology to the Drosophila...

... 2001 © 2001. 2001 Lens Differentiation TGFβ TGFβ receptors Transgenesis Mouse Previous studies have provided evidence that members of the fibroblast growth factor (FGF) and transforming growth factor β (TGFβ) growth factor families play important roles in lens biology. Numerous...

[email protected] ) 14 03 2000 10 05 2000 © 2000 by Company of Biologists 2000 Brachyury Mesoderm Repression SIP1 Transcriptional regulation Transgenesis Xbra Xenopus The Xenopus blastula is patterned into three domains: the animal pole region, the marginal zone...

.... * These authors contributed equally to this manuscript ‡ Author for correspondence (e-mail: [email protected] ) 17 10 1997 © 2000 by Company of Biologists 2000 Xenopus tropicalis Crystallin Lens Timing Transgenesis Differentiation We have used Xenopus tropicalis...

... the regionally specific localisation and activity of these molecules. Xenopus Nodal Xnr1 Organizer Endoderm Transgenesis T-box VegT © 2000 by Company of Biologists 2000 03 01 2000 * Author for correspondence (e-mail: [email protected] ) ...

... zygotically activated gene products, such as the nodal-related proteins ( Jones et al., 1995 ; Joseph and Melton, 1997 ; Smith et al., 1995 ) and derrière ( Sun et al., 1999 ). T-box VegT Endoderm Xenopus laevis Transgenesis Antisense depletion analysis © 1999 by Company of Biologists...

... Evolutionary conservation Pancreas Retina Spinal cord Transgenesis The eye forms during vertebrate embryogenesis through a series of inductive interactions involving neuroectoderm, surface ectoderm, neural crest and mesoderm cell populations. This process, which has long served as a paradigm...

... reporter gene, it should be possible to visualize the origin and migration of any lineage-specific progenitor cells in a living embryo. * Author for correspondence (e-mail: [email protected] ) 06 08 1997 © 1997 by Company of Biologists 1997 transgenesis hematopoiesis zebrafish...