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Keywords: Transgene swapping
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Journal Articles
Hui-Min Chen, Xiaohao Yao, Qingzhong Ren, Chuan-Chie Chang, Ling-Yu Liu, Rosa Linda Miyares, Tzumin Lee
Journal:
Development
Development (2020) 147 (11): dev181974.
Published: 11 June 2020
... gene targeting. Additionally, we built an E-Golic+-based, high-efficiency genetic pipeline for transgene swapping. We demonstrate its utility by transforming GAL4 enhancer-trap lines into tissue-specific Cas9-expressing lines. Given the superior efficiency, specificity and scalability, E-Golic...
Includes: Supplementary data
