... protein to enable accumulation of yolk protein in oocytes during vitellogenesis. MicroRNA Transgene Oogenesis Vitellogenin receptor Bombyx mori National Natural Science Foundation of China 10.13039/501100001809 31530071 WQ 20125500074 An inherently high reproductive...

... fate has not been directly examined. We have used the bacterial artificial chromosome (BAC) modification technique to generate transgenic mice that express DsRed or Cre specifically in NG2-expressing (NG2+) cells. In NG2DsRedBAC transgenic mice, DsRed was expressed specifically in NG2+ cells throughout...

... in independent transgenic lines, they initially appeared healthy. Their oocytes underwent unperturbed meiotic maturation to mII but subsequently exhibited autonomous intracellular free calcium oscillations, second polar body extrusion, pronucleus formation and parthenogenetic development. Transfer of transgenic...

... 2006 © 2006. 2006 Sox10 Neural crest Fate specification Determination Dorsal root ganglion Neurogenin Zebrafish Transgene Waardenburg-Shah syndrome In the trunk and tail, sensory neurons are clustered with support cells(glia) to form a reiterated segmental series...

... myocardin gene, to the hsp68 basal promoter upstream of a lacZ reporter gene and systematically analyzed lacZ expression in F0 transgenic mouse embryos( Fig. 1A ). Only one of the 12 transgenes, containing a DNA fragment located between -32.5 and -22.6 kb upstream of the first exon of the mouse...

... transgene eth3-egfp , where EGFP fluorescence and ETH-like immunoreactivity are colocalized in Inka cells. No other cells exhibit EGFP fluorescence, although some CNS neurons also show ETH-IR. The most intense EGFP fluorescence is observed as larvae approach the first ecdysis, whereas neither EGFP...

... that is expressed in the developing midbrain and is essential for midbrain and anterior hindbrain development. Previous work identified a 5.5 kilobase region located downstream of the Wnt-1 coding sequence which is necessary and sufficient for Wnt-1 expression in vivo. Using a transgenic mouse reporter assay, we...

... and, by analogy, a similar role has been postulated for human ZP3. Genetically altered mice lacking ZP3 ( Zp3 tm / tm ) do not form a zona pellucida and are infertile. To rescue this phenotype, transgenic mice expressing human ZP3 (67% identical to mouse ZP3) were produced and bred with Zp3 tm / tm null mice...

... sequence alignments in parallel with transgenic analyses, followed by in vivo mutagenesis. Here, we report on the identification of a regulatory element that is located in the vicinity of the Hoxd-12 gene. While this element is well conserved in tetrapods, little sequence similarity was scored when...

...-6E.1 genomic fragments to direct expression of a lacZ marker transgene in vivo to study Ly-6E.1 specific regulatory elements in the hematopoietic stem cell and to localize these cells in the developing mouse embryo. We demonstrate that a region approximately 9 kb downstream from the transcriptional...

...Uta Grieshammer; Michael J. McGrew; Nadia Rosenthal ABSTRACT In transgenic mouse embryos, expression of a muscle- specific reporter, consisting of a chloramphenicol acetyl- transferase gene linked to regulatory sequences from the rat myosin light chain 1/3 locus (MLC-CAT), is graded in developing...

... synthesized at the 2-cell stage. * Author for correspondence 12 10 1994 © 1995 by Company of Biologists 1995 zygotic transcription transgene luciferase HSP 70.1 In the mouse embryo, activation of the zygotic genome (ZGA) has been described as a succession of two phases...

...Luisa Dandolo; Colin L. Stewart; Marie-Genevieve Mattei; Philip R. Avner ABSTRACT Transgenes located on the X chromosome have been used to study the mechanisms involved in X-chromosome inactivation. Analysis of the transgenic mouse strain M-TK neo 1 carrying a neomycin resistance gene inserted...

...Maria J. Donoghue; Bruce L. Patton; Joshua R. Sanes; John P. Merlie ABSTRACT We previously used mice bearing a myosin light chainchloramphenicol acetyltransferase (MLC1-CAT) transgene to show that adult muscle cells bear a heritable, cell autonomous memory of their rostrocaudal position. CAT mRNA...

..., timing of X-inactivation and Pgk-1 expression, without the involvement of imprinting at the Pgk-1 locus itself (Fundele R., Illmensee, K., Jagerbauer, E. M., Fehlau, M. and Krietsch, W. K. (1987) Differentiation 35, 31–36). Our findings in transgenic mouse lines, carrying Pgk-1 on autosomes, indicate...

..., researchers have studied methylation phenotypes and expression of hemizygous transgenes associated with parental origin. In this report, we describe a transgenic mouse lineage in which expression of both the transgene and an endogenous gene at the insertion site are determined by the parent of origin...

...-linked transgene, CAT-32. The levels of methylation in embryonic, extra-embryonic and germline cells indicates that the modifications of the transgene are broadly similar to those reported for endogenous X-linked genes. Interestingly, the methylation of CAT-32 transgene in extraembryonic tissues displays...