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Keywords: Transfection
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Journal Articles
Journal: Development
Development (2018) 145 (10): dev162107.
Published: 23 May 2018
... transfection method for the filasterean Capsaspora owczarzaki . We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable organism to use to investigate the origin and evolution of animal multicellularity...
Includes: Supplementary data
Journal Articles
Journal: Development
Development (1997) 124 (10): 1953–1962.
Published: 15 May 1997
... of neuronal differentiation in the neural crest cell population. In fact, transient overexpression of N-myc in the neural crest culture by transfection resulted in a remarkable promotion of neuronal differentiation. An experimental procedure was developed to examine the effect of exogenous N-myc expression...
Journal Articles
Journal: Development
Development (1993) 117 (2): 689–700.
Published: 1 February 1993
...Kathryn Edson; Brigitte Weisshaar; Andrew Matus ABSTRACT We have previously shown that microtubules in non-neuronal cells form long, stable bundles after transfection with the embryonic neuronal microtubule-associated protein MAP2c. In this study, we found that treating MAP2c-transfected cells...
Journal Articles
Journal: Development
Development (1992) 116 (4): 1151–1161.
Published: 1 December 1992
... in non-neuronal cell lines using cell cDNA transfection techniques. At every level of expression, all the MAP2c was bound to cellular microtubules. At low MAP2c levels, the microtubules retained their normal arrangement, radiating from the centrosomal microtubule-organising centre (MTOC) but at higher...