..., the transcriptional dynamics during karyogamy in plant zygotes remain unclear. In this study, we performed a single-cell transcriptome analysis of rice zygotes at six early developmental stages (15 min, 30 min, 1 h, 2 h, 4 h, and 6 h after gamete fusion) to reveal gene expression profiles during karyogamy in plant...

... lineages, the Spiralia, for example, have long been neglected. In order to remedy this shortcoming, we have produced a detailed developmental transcriptome for the bivalve mollusk Mytilus galloprovincialis , and have expanded the list of experimental protocols available for this species. Our high-quality...

..., immune function and cancer progression. As invasion through BM is often stochastic, capturing gene expression profiles of actively invading cells in vivo remains elusive. Using the stereotyped timing of Caenorhabditis elegans anchor cell (AC) invasion, we generated an AC transcriptome during BM breaching...

... 7 9 2022 © 2022. Published by The Company of Biologists Ltd 2022 Summary: The important role of RNA terminal phosphate cyclase B-regulated non-canonical splicing in female reproduction and the mechanisms involved. RNA processing Transcriptome Female fertility Premature...

... vertebrate organogenesis. However, little is known about the cellular heterogeneity of the mesenchymal progenitors in early limb buds that ultimately contribute to the chondrogenic condensations prefiguring the skeleton. We combined flow cytometric and transcriptome analyses to identify the molecular...

... single cell RNA sequencing to profile transcriptomes of dissociated cells from the diencephalon of E12.5 mouse embryos. We identified the divergence of different progenitors, intermediate progenitors, and emerging neurons. By mapping the identified cell groups to their spatial origins, we characterized...

.... However, the cellular and molecular basis of tail regeneration in amphioxus remains largely ill-defined. We confirmed that the tail regeneration of amphioxus Branchiostoma japonicum is a vertebrate-like epimorphosis process. We performed transcriptome analysis of tail regenerates, which provided many...

...; Majlinda Lako ABSTRACT The scarcity of embryonic/foetal material as a resource for direct study means that there is still limited understanding of human retina development. Here, we present an integrated transcriptome analysis combined with immunohistochemistry in human eye and retinal samples from 4 to 19...

... organism. This interest has been further fueled by the advent of high-throughput transcriptomic analyses that provide virtual mountains of data. Here, we present N ematostella vectensis Embryogenesis and Regeneration Transcriptomics (NvERTx), a platform for comparing gene expression during embryogenesis...

... a comprehensive Ciona notochord transcriptome. We identify 1364 genes with enriched expression and extensively validate the results by in situ hybridization. These genes are highly enriched for Gene Ontology terms related to the extracellular matrix, cell adhesion and cytoskeleton. Orthologs of 112 of the Ciona...

... of the A-repeat for the silencing function of Xist RNA in the embryo. Transcriptomic analysis of this mutant trophoblast in comparison with another mutant trophoblast carrying a paternally derived Xist null allele revealed that, although the dysfunctional Xist RNA expressed in the trophoblast indeed failed...

...J. Gray Camp; Barbara Treutlein Innovative methods designed to recapitulate human organogenesis from pluripotent stem cells provide a means to explore human developmental biology. New technologies to sequence and analyze single-cell transcriptomes can deconstruct these ‘organoids’ into constituent...

... or extraembryonic cues. In these simple conditions autocrine signals are sufficient to drive transcriptomic, metabolic and methylome changes that are broadly convergent with peri-implantation epiblast. These findings indicate that the gene regulatory circuitry of ES cells has an innate capacity to orchestrate...

... in maize anthers, using proteins conserved in Arabidopsis and rice but deployed with distinctive timing. Pre-meiotic male reproduction Regulatory hierarchy Transcriptome Proteome Maize is monoecious, with separate inflorescences producing female-only ears and male-only tassels...

... and spatial gene expression changes occurring during rice embryogenesis are captured. Embryogenesis Rice Transcriptome Gene expression Microarray Microdissection In higher plants, embryogenesis is a process that establishes the body plan required for early vegetative growth after...

... the same embryo. As predicted by the transcriptomic data, immunolocalization of EOMES, T brachyury, GDF15 and active β-catenin revealed differential expression among blastomeres of 8- to 10-cell human embryos. The UCSFB lines formed derivatives of the three germ layers and CDX2-positive progeny, from which...

... for correspondence ( [email protected] ; [email protected] ) Competing interests The authors declare no competing financial interests. © 2014. Published by The Company of Biologists Ltd 2014 Body plan Evo-devo Evolution Hourglass model Phylotype Transcriptome Animals...

... identified. We have undertaken a transcriptome analysis of mouse limb tendon cells that were isolated at different stages of development based on scleraxis ( Scx ) expression. Microarray comparisons allowed us to establish a list of genes regulated in tendon cells during mouse limb development...

... of transcriptome change in progressively more differentiated neural cell populations in vivo . Microarray data, validated against direct RNA sequencing, identified: (1) a gene cohort characteristic of the multi-potent stem zone epiblast, which contains neuro-mesodermal progenitors that progressively generate...

... requires the identification of crucial differentially expressed control genes and, ideally, the determination of the complete transcriptomes of each individual cell type. Here, we have analyzed the transcriptomes of six major tissues dissected from mid-gestational (TS12) mouse embryos. Approximately one...