... investigated a K5.Foxn1 transgene that drives over-expression in TECs, but neither causes hyperplasia, nor delays or prevents aging-related involution. Similarly, this transgene cannot rescue thymus size in Foxn1 lacZ/lacZ mice that undergo premature involution due to reduced Foxn1 levels. However, K5.Foxn1...

...Pedro Ferreirinha; Ruben G. R. Pinheiro; Jonathan J. M. Landry; Nuno L. Alves ABSTRACT The thymus stroma constitutes a fundamental microenvironment for T-cell generation. Despite the chief contribution of thymic epithelial cells, recent studies emphasize the regulatory role of mesenchymal cells...

... population. Scatter plot shows the percentage of CD62L + cells in the CD69 − CD4SP population. (D) Percentage of Tregs (CD8 − CD4 + CD25 + icFoxp3 + ) in the control and Foxa1/2cKO thymus. (E) Percentage of NK (CD3 − NK1.1 + ) and NKT cells (CD3 + NK1.1 + ) in the control and Foxa1/2cKO thymus. (F,G) Flow...

.... and Zúñiga-Pflücker , J. C. ( 2019 ). RBPJ-dependent Notch signaling initiates the T cell program in a subset of thymus-seeding progenitors . Nat. Immunol.   20 , 1456 - 1468 . 10.1038/s41590-019-0518-7 Ci , W. , Polo , J. M. , Cerchietti , L. , Shaknovich , R. , Wang , L...

...Dong Liu; Anastasia I. Kousa; Kathy E. O'Neill; Paul Rouse; Martyna Popis; Alison M. Farley; Simon R. Tomlinson; Svetlana Ulyanchenko; Francois Guillemot; Philip A. Seymour; Mette C. Jørgensen; Palle Serup; Ute Koch; Freddy Radtke; C. Clare Blackburn ABSTRACT Thymus function depends...

.... S. and Boehm , T. ( 2006 ). Formation of a functional thymus initiated by a postnatal epithelial progenitor cell . Nature   441 , 992 - 996 . 10.1038/nature04850 Chen , E. L. Y. , Thompson , P. K. and Zuniga-Pflucker , J. C. ( 2019 ). RBPJ-dependent Notch signaling...

...Virginia E. Bain; Julie Gordon; John D. O'Neil; Isaias Ramos; Ellen R. Richie; Nancy R. Manley The thymus and parathyroids develop from third pharyngeal pouch (3rd pp) endoderm. Our previous studies show that S hh null mice have smaller, aparathyroid primordia in which thymus fate specification...

.... Previous studies have shown that the Hoxa3 null mutant lacks third pharyngeal pouch derivatives, the thymus and parathyroids by E18.5, and organ-specific markers are absent or downregulated during initial organogenesis. Our current analysis of the Hoxa3 null mutant shows that organ-specific domains did...

...Kaitlin A. G. Reeh; Kim T. Cardenas; Virginia E. Bain; Zhijie Liu; Micheline Laurent; Nancy R. Manley; Ellen R. Richie The thymus and parathyroid glands arise from a shared endodermal primordium in the third pharyngeal pouch (3rd pp). Thymus fate is specified in the ventral 3rd pp between E9.5...

...Jennifer R. Gardiner; Abigail L. Jackson; Julie Gordon; Heiko Lickert; Nancy R. Manley; M. Albert Basson The thymus and parathyroid glands are derived from the third pharyngeal pouch endoderm. The mechanisms that establish distinct molecular domains in the third pouch and control the subsequent...

.../mesenchymal interaction in the early fetal thymus . Eur J. Immunol. 27 , 522 - 529 . Smith C. ( 1965 ). Studies on the thymus of the mammal XIV: Histology and histochemistry of embryonic and ealy postnatal thymuses of C57BL/6 and AKR strain mice . Am. J. Anat. 116 , 611 - 630...

...Tadashi Okubo; Akinori Kawamura; Jun Takahashi; Hisato Yagi; Masae Morishima; Rumiko Matsuoka; Shinji Takada The pharyngeal apparatus is a transient structure that gives rise to the thymus and the parathyroid glands and also contributes to the development of arteries and the cardiac outflow tract...

.... We show that some organs, e.g. the thymus, require Tbx1 expression in the mesoderm and in the epithelia. In addition, these experiments revealed that different pharyngeal arches require Tbx1 in different tissues. Finally, we show that Tbx1 in the mesoderm is required to sustain cell proliferation...

... ). As fetal thymocytes normally use γ/δ T cell receptors, it was surprising to find that TCRαβ lymphocytes predominated in co-cultures initiated with P-Sp/AGM cells. However, addition of IL7 to the cultures enhanced production of TCRγδ + cells. Cytokine concentrations in early fetal versus later thymuses...

... and parathyroid glands ( Fig. 4H , Table 1 , Table 3 ). Table 3. Glandular phenotypes of Fgf8 domain-specific mutants: sectioned specimens at E18.5 Genotype Normal thymus Ectopic thymus Hypoplastic thymus Normal parathyroid glands * Ectopic parathyroid glands Hypoplastic parathyroid...

.... H. Ingbar), pp. 303-317. New York: Harper and Row. Smith, C. ( 1965 ). Studies on the thymus of the mammal XIV: Histology and histochemistry of embryonic and early postnatal thymuses of C57BL/6 and AKR strain mice. Am. J. Anat . 116 , 611 -630. Strachan, T. and Read, A. P. ( 1994 ). PAX...

... . Int. Immunol . 7 , 435 – 448 . 10.1093/intimm/7.3.435 Wekerle , H. , Ketelsen , U.-P. and Ernst , M. ( 1980 ). Thymic nurse cells: Lymphoepithelial cell complexes in murine thymuses. Morphological and serological characterization . J. Exp. Med . 151 , 925 – 944...

... differentiation of the surrounding pharyngeal arch and pouch tissues. Hoxa-3 mutant mice are athymic and show thyroid hypoplasia. Thymus development is first evident as an expansion of mesenchymal neural crest in the posterior part of the 3rd pharyngeal pouch. Prior to this expansion, a marked reduction in pax-1...

... of thymic stromal cell types are retained and that increased numbers of cysts, mucous and myoid cells are found in the thymus following wholebody irradiation. In vivo-irradiated thymuses are therefore suitable for implantation studies exploring the role of thymic stromal cells in tolerance induction...