... fates. Genetics of sex Ovotestis Sex determination Sex reversal Testis Znrf3 Mouse Medical Research Council http://dx.doi.org/10.13039/501100007155 MC_UP_2201/1 MC_U142684167 Mammalian sex determination is the process by which the bipotential fetal gonad primordium...

... have informed our understanding of typical spermatogenesis, the molecular and cellular mechanisms underlying the maintenance and decline of spermatogenesis are not yet understood. We used single-cell RNA sequencing to generate a developmental atlas of the aging zebrafish testis. All testes contained...

...Chaofan Jin; Kai Yan; Mengya Wang; Weihao Song; Bo Wang; Yu Men; Jingjing Niu; Yan He; Quanqi Zhang; Jie Qi ABSTRACT Teleost testis development during the annual cycle involves dramatic changes in cellular compositions and molecular events. In this study, the testicular cells derived from adult...

... in the testis after sex determination, we analyzed mice lacking NR5A1 in Sertoli cells (SCs) from embryonic day (E) 13.5 onwards. Ablation of Nr5a1 impaired the expression of genes characteristic of SC identity (e.g. Sox9 and Amh ), caused SC death from E14.5 onwards through a Trp53 -independent mechanism...

... substance’. However, evidence for this role comes from studies in the fetal ovary, where germ cell differentiation and meiotic initiation are temporally inseparable. In the postnatal testis, these events are separated by more than 1 week. Exploiting this difference, we discovered that, although RA...

... and Tcf21-CreER + stromal cells in the testis, but Sertoli cells are the only physiologically important source of SCF that contributes to spermatogenesis. Spermatogenesis Stem cell factor Sertoli cell Testis Mammalian spermatogenesis is a complex developmental process based on a robust...

... . 10.3109/08977199509036882 Hiramatsu , R. , Kanai , Y. , Mizukami , T. , Ishii , M. , Matoba , S. , Kanai-Azuma , M. , Kurohmaru , M. , Kawakami , H. and Hayashi , Y. ( 2003 ). Regionally distinct potencies of mouse XY genital ridge to initiate testis...

... initiation in vitro and isolate pure populations of millions of cells undergoing the mitosis-to-meiosis transition for biochemical assays. Testis Spermatogenesis Meiosis Spermatogonia Spermatocytes National Institutes of Health http://dx.doi.org/10.13039/100000002 HD090083 HD090007...

... were then added to cell lysate overnight at 4°C. Before performing the western blot, beads were washed five times with PBS. The expressions of Sohlh1 and Sohlh2 were detected by in situ hybridization with digoxigenin (DIG)-labeled probes on frozen testis sections. The slides were fixed...

... of proteins mediates movement of specific cargo proteins when bound to karyopherin β. Karyopherin α genes have distinct expression patterns in mouse testis, implying they may have unique roles during mammalian spermatogenesis. Here, we use a loss-of-function approach to determine specifically the role...

... by The Company of Biologists Ltd 2020 http://www.biologists.com/user-licence-1-1/ Summary: Zebrafish Nedd8 plays an important role in controlling ovarian maturation through suppressing androgen receptor activity. Nedd8 Zebrafish Androgen receptor Ovary Testis Neddylation...

...Aya Uchida; Sadman Sakib; Elodie Labit; Sepideh Abbasi; R. Wilder Scott; T. Michael Underhill; Jeff Biernaskie; Ina Dobrinski ABSTRACT In mammalian testis, contractile peritubular myoid cells (PMCs) regulate the transport of sperm and luminal fluid, while secreting growth factors and extracellular...

...-cell RNA sequencing Pro-spermatogonia Gonocyte Spermatogonial stem cell Sertoli cells Peritubular myoid cell Leydig cells Testis National Institutes of Health 10.13039/100000002 R01 GM119128 Lalor Foundation 10.13039/100012756 Pro-spermatogonia (ProSG) – also...

...John E. La Marca; Sarah T. Diepstraten; Amy L. Hodge; Hongyan Wang; Adam H. Hart; Helena E. Richardson; W. Gregory Somers ABSTRACT One fundamental property of a stem cell niche is the exchange of molecular signals between its component cells. Niche models, such as the Drosophila melanogaster testis...

...Ellen K. Velte; Bryan A. Niedenberger; Nicholas D. Serra; Anukriti Singh; Lorena Roa-DeLaCruz; Brian P. Hermann; Christopher B. Geyer; Allon Klein; Barbara Treutlein ABSTRACT In the mammalian testis, sustained spermatogenesis relies on spermatogonial stem cells (SSCs); their progeny either remain...

... 2018 © 2019. Published by The Company of Biologists Ltd 2019 Summary: This Primer reviews the role of JAK/STAT signaling in stem cells and regeneration in Drosophila testis, intestine and appendages, and discusses the similarities between Drosophila and vertebrates. Drosophila...

... relationship between FLCs and ALCs has not been clarified yet. Here, we reveal that a subset of FLCs dedifferentiate at fetal stages to give rise to ALCs at the pubertal stage. Moreover, the dedifferentiated cells contribute to the peritubular myoid cell and vascular pericyte populations in the neonatal testis...

.../ Summary: Dynamic cytoplasmic projections connect mammalian spermatogonia and provide patent connections for the sharing of macromolecules during testis development. TEX14 Intercellular bridge Spermatogenesis Spermatogonia Testis Mouse Baboon Eunice Kennedy Shriver National Institute...

.... Additionally, blocking RA signaling in Leydig cells resulted in increased permeability of the blood-testis barrier, decreased levels of the steroidogenic enzyme cytochrome P450 17a1 and decreased testosterone levels. Surprisingly, the epididymides of the mutant mice also displayed an abnormal phenotype...

... cyst stem cells (CySCs) in the Drosophila testis is actively promoted by PI3K/Tor signaling, as CySCs lacking PI3K/Tor activity cannot differentiate properly. We find that an insulin peptide produced by somatic cells immediately outside of the stem cell niche acts locally to promote somatic...