...Deepak Jain; Stephen Nemec; Maëva Luxey; Yves Gauthier; Amandine Bemmo; Aurelio Balsalobre; Jacques Drouin ABSTRACT In tetrapods, Tbx4 , Tbx5 and Hox cluster genes are crucial for forelimb and hindlimb development and mutations in these genes are responsible for congenital limb defects...

... Expression profiling ChIPseq Tbx5 Mouse Canadian Institutes of Health Research 10.13039/501100000024 MOP-123213 CEEHRC EP1-120608 In tetrapods, forelimbs (FLs) and hindlimbs (HLs) develop complex musculoskeletal structures within the framework of a common three-segment organization...

...Carolina Minguillon; Satoko Nishimoto; Sophie Wood; Elisenda Vendrell; Jeremy J. Gibson-Brown; Malcolm P. O. Logan Tbx4 and Tbx5 are two closely related T-box genes that encode transcription factors expressed in the prospective hindlimb and forelimb territories, respectively, of all jawed...

...Peleg Hasson; Joanne Del Buono; Malcolm P. O. Logan Tbx5 is essential for initiation of the forelimb, and its deletion in mice results in the failure of forelimb formation. Misexpression of dominant-negative forms of Tbx5 results in limb truncations,suggesting Tbx5 is also required for forelimb...

... indistinguishable from Tbx4 null embryos and died at E10.5 due to failure of chorioallantoic fusion (data not shown). * Author for correspondence (e-mail: [email protected] ) 24 10 2006 © 2007. 2007 Tbx4 Hindlimb Limb T-box Tbx5 Fgf10 Mouse Vertebrate limbs consist...

... regulatory hierarchy has not been determined in any species. tbx5 is the earliest gene expressed in the limb bud mesenchyme. Recently, another transcription factor, Prdm1, has been shown to be crucial for zebrafish forelimb development. Here, we show that Prdm1 is downstream of RA, Wnt2b and Tbx5 activity...

...Sarah C. Goetz; Daniel D. Brown; Frank L. Conlon Despite the critical importance of TBX5 in normal development and disease,relatively little is known about the mechanisms by which TBX5 functions in the embryonic heart. Our present studies demonstrate that TBX5 is necessary to control the length...

... signaling is not required for the establishment or maintenance of dorsoventral patterning in the retina, as we observe normal expression of Tbx5 and ephrin B2 ( Efnb2 )dorsally, plus Vax2 and Ephb2 ventrally. Instead, RA is required for the morphogenetic movements needed to shape the developing retina...

... in the T-box transcription factor TBX5 cause Holt-Oram syndrome (HOS), which results in forelimb and heart defects. Although mutations in TBX5 result in HOS, it has been predicted that these mutations account for only ∼30% of all individuals with HOS. Individuals with OS and HOS limb defects are very...

... the synergistic activity of key cardiac transcription factors, Nkx2.5 and Tbx5, leading to reduced activation of the common target gene, Anf ( Nppa ). Strikingly, reduced levels of Anf expression were also observed in embryonic day 9.5 Carp-Lbh transgenic mice. Thus, repression of Nkx2.5 and Tbx5-mediated gene...

... 14 12 2004 ©2005. 2005 Tbx5 Gata4 Nkx2-5 Smooth muscle Transgenesis Knowledge of the regulatory mechanisms underlying cardiac development is essential for understanding the molecular basis of congenital heart defects. During embryonic heart development, differentiation...

... and the mechanism by which individual T-box genes function remains largely unknown. In this study, we have investigated the cellular and molecular relationship between two T-box genes, Tbx5 and Tbx20 . We demonstrate that blocking Tbx5 or Tbx20 produces phenotypes that display a high degree of similarity, as judged...

...Ivan P. G. Moskowitz; Anne Pizard; Vickas V. Patel; Benoit G. Bruneau; Jae B. Kim; Sabina Kupershmidt; Dan Roden; Charles I. Berul; Christine E. Seidman; Jonathan G. Seidman We report a critical role for the T-box transcription factor Tbx5 in development and maturation of the cardiac conduction...

...Jun K. Takeuchi; Makoto Ohgi; Kazuko Koshiba-Takeuchi; Hidetaka Shiratori; Ichiro Sakaki; Keiko Ogura; Yukio Saijoh; Toshihiko Ogura Extensive misexpression studies were carried out to explore the roles played by Tbx5 , the expression of which is excluded from the right ventricle (RV) during...

...Sabine Fischer; Bruce W. Draper; Carl J. Neumann The development of vertebrate limb buds is triggered in the lateral plate mesoderm by a cascade of genes, including members of the Fgf and Wnt families,as well as the transcription factor tbx5 . Fgf8, which is expressed in the intermediate mesoderm...

...Charalampos Rallis; Benoit G. Bruneau; Jo Del Buono; Christine E. Seidman; J. G. Seidman; Sahar Nissim; Clifford J. Tabin; Malcolm P. O. Logan Tbx5 is a T-box transcription factor expressed exclusively in the developing forelimb but not in the developing hindlimb of vertebrates. Tbx5 is first...

...Jun K. Takeuchi; Kazuko Koshiba-Takeuchi; Takayuki Suzuki; Mika Kamimura; Keiko Ogura; Toshihiko Ogura A tight loop between members of the fibroblast growth factor and the Wnt families plays a key role in the initiation of vertebrate limb development. We show for the first time that Tbx5 and Tbx4...

... of interplay between fibroblast growth factor(FGF) and Wnt signaling. What initiates these signaling cascades and thus limb bud outgrowth at defined locations along the anteroposterior axis of the embryo is not known. The T-box transcription factor TBX5 is important for normal heart and limb formation, but its...

... of tissues and organs are linked during embryogenesis. The vertebrate limb is one of the best models to study these processes. Combining mutant analyses with gain- and loss-of-function approaches in zebrafish and chick embryos, we show that Tbx5 , in addition to its role governing forelimb identity,is both...

... of tbx5 , a gene required for normal heart morphogenesis. Conversely, we show that overexpression of hrT causes a significant downregulation of tbx5 , indicating that one key role of hrT is to regulate the levels of tbx5 . Secondly, we found that HrT is required to inhibit the expression of the blood...