...Jin Ben; Stone Elworthy; Ashley Shu Mei Ng; Freek van Eeden; Philip W. Ingham Using zinc-finger nuclease-mediated mutagenesis, we have generated mutant alleles of the zebrafish orthologue of the chicken talpid3 ( ta3 ) gene, which encodes a centrosomal protein that is essential for ciliogenesis...

... are essential to rescue cilia in talpid 3 chicken mutants. We constitutively deleted these two exons to make a talpid3 –/– mouse. Mutant mouse embryos lack primary cilia and, like talpid 3 chicken embryos, have face and neural tube defects but also defects in left/right asymmetry. Conditional deletion in mouse...

... 3 mutant embryos lack primary cilia and that primary cilia can be rescued with constructs encoding Talpid3. talpid 3 mutant embryos also develop polycystic kidneys,consistent with widespread failure of ciliogenesis. Ultrastructural studies of talpid 3 mutant neural tube show that basal bodies mature...