...Lorna Taylor; Daniel F. Carlson; Sunil Nandi; Adrian Sherman; Scott C. Fahrenkrug; Michael J. McGrew In this work we use TALE nucleases (TALENs) to target a reporter construct to the DDX4 ( vasa ) locus in chicken primordial germ cells (PGCs). Vasa is a key germ cell determinant in many animal...

... TALEN and other TAL effector-based constructs for DNA targeting . Nucleic Acids Res.   39 , e82 . 10.1093/nar/gkr218 Chauveau , C. , Rowell , J. and Ferreiro , A. ( 2014 ). A rising titan: TTN review and mutation update . Hum. Mutat.   35 , 1046 - 1059 . 10.1002/humu.22611...

... by The Company of Biologists Ltd 2016 Summary: Genome-editing approaches in zebrafish reveal that distinct signaling pathways downstream of Flt4 govern lymphatic morphogenesis and differentiation in different anatomical locations. Flt4 TALEN Vegfc Lymphatic Zebrafish National Heart...

... generated zebrafish mutants lacking all four zebrafish Mesp genes by using TALEN-mediated genome editing. Contrary to the situation in the mouse Mesp2 mutant, in the zebrafish Mesp quadruple mutant embryos the positions of somite boundaries were clearly determined and morphological boundaries were formed...

... in vivo , but it also paves the way for other sophisticated genetic manipulations of the zebrafish genome. TALEN Genome engineering Homologous recombination Knock-in sox2 gfap Fig. 7. A schema of the reported strategy for efficient HR-mediated genome editing in zebrafish. Co...