... cells, alternative RNA splicing was dysregulated in the double Foxa1/Foxa2 conditional knockout, leading to >850 differentially used exons. Many genes important for this stage of T-cell development ( Ikzf1-3 , Ptprc , Stat5a , Stat5b , Cd28 , Tcf7 ) and splicing factors ( Hnrnpab , Hnrnpa2b1 , Hnrnpu...

...-positive cells in the thymus, and attenuates Notch1 signalling after β-selection. Pre-TCR Bcl6 Notch Thymus Thymocyte T-cell development αβT cells develop in the thymus, which provides an essential environment for T-cell fate specification and differentiation ( Koch and Radtke, 2011...

... in thymic epithelial cells promotes maturation from CD4 + CD8 + to CD4 + CD8 − thymocyte by reducing Shh, which signals to reduce T-cell receptor-mediated transcription during repertoire selection in mouse fetal thymus. Shh Gli3 Fetal thymus Positive selection CD4 T-cell development...

..., but when transfected to express the Notch ligand Delta-like1 (DL1), OP9-DL1 cells support T-cell development ( Schmitt and Zúñiga-Pflücker, 2002 ). Thus, thymocytes were infected with empty vector or with PU.1-expressing retrovirus during a 4-hour incubation, cultured with OP9-DL1 or OP9-control stroma...