...-right asymmetry Nodal signaling Squint Mature domain Pro-domain Zebrafish Cyclops Intercellular signaling plays crucial roles not only in establishing the body plan and forming the germ layers during embryonic development, but also in cancer progression. For example, formation...

...). The zebrafish Nodal signal Squint functions as a morphogen. Nature 411 , 607 -610. Chomczynski, P. and Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 , 156 -159. Ciccodicola, A., Dono, R., Obici, S., Simeone...

... of neural tissue in zebrafish wild-type and Nodal-deficient embryos (Antivin overexpressing or cyclops;squint double mutants), which show extensive AP brain patterning in the absence of dorsal mesoderm. In different genetic situations that vary the extent of mesodermal precursor formation, the presence...

... encoding the nodal-related factor, Squint, and homeodomain protein, Bozozok, both of which are expressed in the YSL, suggested that the YSL has a role in mesendoderm induction. However, these genes, and a second nodal -related factor, cyclops , are also expressed in the overlying marginal blastomeres...

... of dorsal axial structures are the homeobox gene bozozok (boz) and the nodal-related (TGF-P family) gene squint (sqt) . Both genes are expressed in the dorsal yolk syncytial layer, a source of dorsal mesoderm inducing signals, and mutational analysis has indicated that boz and sqt are required for dorsal...

... squint and cyclops , members of the nodal group of TGF-β related molecules, gata5 expression in the blastoderm is abolished, making these factors primary candidates for the endogenous TGF-β signal inducing gata5 . This paper is dedicated to the memory of Nigel Holder who died tragically...