... identified AMG-1 as an mtRBP required for reproductive success in Caenorhabditis elegans . amg-1 mutation led to defects in mitochondrial structure and sperm budding, resulting in mitochondria being discarded into residual bodies, which ultimately delayed spermatogenesis in the proximal gonad. In addition...

... structures depending on sequence and length ( Jain and Vale, 2017 ; Tauber et al., 2020 ; Van Treeck et al., 2018 ). Several perinuclear condensates, including Mutator foci and P granules in C. elegans , and the chromatoid body of mammalian sperm, assemble near nuclear pore complexes and disassemble when...

...Xiao-Zhen Zhang; Lin-Lin Wei; Xiao-Hui Zhang; Hui-Juan Jin; Su-Ren Chen ABSTRACT The perinuclear theca (PT) is a cytoskeletal element encapsulating the sperm nucleus; however, the physiological roles of the PT in sperm are largely uncertain. Here, we reveal that ACTRT1, ACTRT2, ACTL7A and ACTL9...

... abnormalities of the sperm flagella (MMAF)-associated genes lead to decreased sperm motility and impaired male fertility. As an MMAF gene, the function of fibrous sheath-interacting protein 2 ( FSIP2 ) remains largely unknown. In this work, we identified a homozygous truncating mutation of FSIP2 in an infertile...

...Mengyun Wang; Yue Du; Song Gao; Zheng Wang; Pengxiang Qu; Yang Gao; Jingyi Wang; Zhengqi Liu; Jingcheng Zhang; Yong Zhang; Suzhu Qing; Yongsheng Wang ABSTRACT In mammals, sperm-borne regulators can be transferred to oocytes during fertilization and have different effects on the formation...

...Daniela R. Chavez; Angela K. Snow; Joseph R. Smith; Gillian M. Stanfield ABSTRACT The development of highly differentiated sperm cells that are specialized for navigating to and fusing with an oocyte is essential for sexual reproduction. As a major part of differentiation, sperm undergo extensive...

...Alaa Hachem; Jonathan Godwin; Margarida Ruas; Hoi Chang Lee; Minerva Ferrer Buitrago; Goli Ardestani; Andrew Bassett; Sebastian Fox; Felipe Navarrete; Petra de Sutter; Björn Heindryckx; Rafael Fissore; John Parrington ABSTRACT Activation of the egg by the sperm is the first, vital stage...

...Shuiqiao Yuan; Andrew Schuster; Chong Tang; Tian Yu; Nicole Ortogero; Jianqiang Bao; Huili Zheng; Wei Yan Although it is believed that mammalian sperm carry small noncoding RNAs (sncRNAs) into oocytes during fertilization, it remains unknown whether these sperm-borne sncRNAs truly have any function...

... are impaired. Instead, we found that impairing P granules causes sperm-specific mRNAs to become highly overexpressed. This includes the accumulation of major sperm protein (MSP) transcripts in germ cells, a phenotype that is suppressed by feminization of the germline. A core component of P granules, the endo...

...Hitomi Watanabe; Toru Takeo; Hiromasa Tojo; Kazuhito Sakoh; Thorsten Berger; Naomi Nakagata; Tak W. Mak; Gen Kondoh Mammalian sperm undergo multiple maturation steps after leaving the testis in order to become competent for fertilization, but the molecular mechanisms underlying this process remain...

... patterns of the corresponding regions in sperm and oocytes. We found that the extent of the gametic DMRs differs significantly from that of the embryonic DMRs, especially in the case of paternal gametic DMRs. These results suggest that the gametic DMR sequences should be used to extract the features...

... the actions of NO and GSNO on [Ca 2+ ] i . The effects of NO, GSNO and dithiothreitol on sperm protein S-nitrosylation, assessed using the biotin switch method, closely paralleled their actions on[Ca 2+ ] i . Immunofluorescent staining revealed constitutive and inducible NOS in human oviduct and cumulus...

... hyaluronan fragments, treated them with purified hyaluronidase or exposed them to sperm as a physiologically relevant source of hyaluronidase. Hyaluronan fragments or hyaluronidase activated the NFκB pathway and induced Il6, Ccl4 and Ccl5 mRNA expression within 2 hours. Anti-TLR2 and anti-TLR4 neutralizing...

...Kiera von Besser; Aubrey C. Frank; Mark A. Johnson; Daphne Preuss In flowering plants, sperm cells develop in the pollen cytoplasm and are transported through floral tissues to an ovule by a pollen tube, a highly polarized cellular extension. After targeting an ovule, the pollen tube bursts...

... family member. Previous studies have shown that FOG-1 specifies the sperm fate and that FBF promotes proliferation. Here, we report that FOG-1 also promotes proliferation. Whereas fbf-1 fbf-2 double mutants make ∼120 germ cells, fog-1; fbf-1 fbf-2 triple mutants make only ∼10 germ cells. The triple...

...Indrani Chatterjee; Alissa Richmond; Emily Putiri; Diane C. Shakes; Andrew Singson A mutation in the Caenorhabditis elegans spe-38 gene results in a sperm-specific fertility defect. spe-38 sperm are indistinguishable from wild-type sperm with regards to their morphology, motility and migratory...

...Carey Rodeheffer; Barry D. Shur Mammalian sperm must undergo a physiological maturation, termed capacitation, before they are able to fertilize eggs. Despite its importance,the molecular mechanisms underlying capacitation are poorly understood. In this paper, we describe the capacitation phenotype...

...Carey Rodeheffer; Barry D. Shur During mammalian fertilization, sperm adhere to the extracellular coat of the egg, or zona pellucida, in a species-specific manner. In mouse, evidence suggests that sperm recognize and bind to specific oligosaccharide ligands within the zona pellucida glycoprotein...

..., later dividing cell, contributes mainly to the abembryonic part. We have previously observed that both the plane of this first cleavage and the subsequent order of blastomere division tend to correlate with the position of the fertilisation cone that forms after sperm entry. But does sperm entry...

... of mammalian embryos. Indeed it now appears that there are at least two spatial cues that operate in the mouse egg to shape polarity of the blastocyst. One of these is at the animal pole, which is defined by the site of female meiosis, and another is associated with the position of sperm entry. In this review...