... show that zebrafish Tbx16 (Spadetail) is capable of advancing mesodermal differentiation cell-autonomously. Tbx16 locks cells into the mesodermal state by not only activating downstream mesodermal genes, but also by repressing bipotential progenitor genes, in part through a direct repression of sox2...

.... 19 7 2013 © 2013. Published by The Company of Biologists Ltd 2013 Fgf2 Kupffer’s vesicle Left-right patterning Syndecan 2 Spadetail Heparan sulfate proteoglycans (HSPGs) comprise a core protein with covalently attached heparan sulfate glycosaminoglycans (HS-GAGs...

... Limb Pectoral fin Somites Spadetail No tail Neckless Zebrafish Vertebrate limbs arise bilaterally from the lateral plate mesoderm (LPM) of the embryo. Within the trunk LPM, interactions between the limb bud mesenchyme and the overlying ectoderm result in the initiation of local patterning...

... in spadetail mutants that have very reduced somitic mesoderm, in no tail mutants injected with spadetail morpholino antisense oligonucleotides that entirely lack somitic mesoderm and in somite segmentation mutants that have normal somite components but disrupted segment borders. Fast muscle cells appear...

... ) * Present address: Department of Anatomy, Cambridge University, Downing Street, Cambridge CB2 3DY, UK 12 11 2003 © 2004. 2004 Somites Motoneurons MiP CaP Zebrafish Spinal cord Trilobite Knypek No tail Spadetail Fused somites You-too After eight b380 b567...

... posterior mesoderm. Thus, fgf8 and fgf24 are together required to promote posterior mesodermal development. We provide both phenotypic and genetic evidence that these Fgf signaling components interact with no tail and spadetail , two zebrafish T-box transcription factors that are required...

... mediator of FGF signaling in the notochord and tail, the pathways activated by FGF in non-notochordal trunk mesoderm have been uncertain. Previous studies have shown that the spadetail gene is required for non-notochordal trunk mesoderm formation; spadetail mutant embryos have major trunk mesoderm...

... with a dominant-negative secreted construct suggest that papc is required for proper dorsal convergence movements during gastrulation. Genetic studies show that papc is a close downstream target of spadetail , gene encoding a transcription factor required for mesodermal morphogenetic movements. Further, we show...

... in floating head mutant gastrulae fails to maintain expression of notochord genes and instead expresses muscle genes. Zebrafish spadetail mutant gastrulae have a nearly opposite phenotype; notochord markers are expressed in a wider domain than in wild-type embryos and muscle marker expression is absent. We...

..., extension movements on the dorsal side of the embryo are affected, whereas in the formerly described spadetail mutants, for which two new alleles have been isolated, convergent movements of ventrolateral cells to the dorsal side are blocked. Two genes are required for the development of the posterior end...

... of the zebrafish snail1 gene and its expression in wild-type, spadetail and no tail mutant embryos . Development 119 , 1203 – 1215 . 10.1242/dev.119.4.1203 Toyama , R. , O’Connell , M. L. , Wright , C. V. E. , Kuehn , M. R. and Dawid , I. B. ( 1995 ). Nodal induces...

... increases in cells that define the posterior border of each somite and then disappears when somitic cells differentiate. Later in development, expression appears in cephalic neural crest derivatives. Many snail1 -expressing cells were missing from mutant spadetail embryos and the quantity of snail1 RNA...

..., resulting in embryos with exaggerated dorsoanterior structures. In LiCl-treated embryos, eve1 transcripts are completely absent. eve1 is therefore a marker of ventral and posterior cells. In the light of its ventroposterior expression domain, the localization of eve1 transcripts was analysed in spadetail...

... are themselves responsible for establishing this morphological dorsoventral boundary (see Discussion). A mutation, spt-l(b104) , or ‘spadetail’, allows further examination of this proposal, since spt-1 severely perturbs somitogenesis in the trunk, and the myotomes that eventually form are disorganized...