...% of these mice had no sperm in the left epididymis. Wnt4 -cKO mice had fertility defects and produced 52% fewer litters and 78% fewer pups than control mice. Wnt4- cKO testes demonstrated increased expression of estrogen receptor α and SOX9, upregulation of female gonadal genes, and a decrease in male gonadal...

... for the formation of a fully mature functional lung at birth. Sox9 + cells present at the tip of the primary embryonic lung endoderm are multipotent cells responsible for branch formation and elongation. We performed a genetic screen in murine primary cells and identified aurora kinase b (Aurkb) as an essential...

... bone. Despite their importance, the mechanism by which superstructures are patterned is unknown. Here, we identify components of the genetic program that control the patterning of Sox9 + / Scx + superstructure progenitors in mouse and show that this program includes both global and regional regulatory...

... of bones has been challenging. Here, we compare our previously reported mechanisms of patella development in the mouse with those of two anatomically different sesamoids, namely lateral fabella and digit sesamoids. We show that all three types of sesamoid bones originate from Sox9 + / Scx + progenitors...

...Neta Felsenthal; Sarah Rubin; Tomer Stern; Sharon Krief; Deepanwita Pal; Brian A. Pryce; Ronen Schweitzer; Elazar Zelzer ABSTRACT Tendon-bone attachment sites, called entheses, are essential for musculoskeletal function. They are formed embryonically by Sox9 + progenitors and continue to develop...

... pigmented epithelium (RPE). Here, we describe a novel regulatory relationship between the RPE transcription factors Pax6 and Sox9 that controls the timing of RPE differentiation and the adjacent choroid maturation. We used a novel machine learning algorithm tool to analyze high resolution imaging...

... Sox9, an important transcription factor of skeletogenesis. We further demonstrate that Erk5 directly phosphorylates and activates Smurf2 (a ubiquitin E3 ligase) at Thr 249 , which promotes the proteasomal degradation of Smad proteins and phosphorylates Smad1 at Ser 206 in the linker region known...

...Chia-Feng Liu; Marco Angelozzi; Abdul Haseeb; Véronique Lefebvre ABSTRACT SOX9 controls cell lineage fate and differentiation in major biological processes. It is known as a potent transcriptional activator of differentiation-specific genes, but its earliest targets and its contribution to priming...

... normally in the arches of sox9a mutants ( Fig. S6A ). However, expression of the Sox9 target genes col2a1a , matn1 and matn4 , and to a lesser extent acana , was specifically reduced in the nascent sox9a + upper facial cartilages affected in foxc1a ; foxc1b mutants ( Fig. 5 B). The exact...

... chondrocyte proliferation and differentiation/maturation, leading to chondrodysplasia. These defects were linked to suppression of SOX9, a master regulator of chondrogenesis, the expression of which is mediated by TEAD transcription factors. Our data indicate that a MOB1-dependent YAP1/TAZ-TEAD complex...

... of the embryonic thyroid gland in mice. Sox9, a key factor in branching organ development, distinguishes Nkx2-1 + cells in the thyroid bud from the progenitors that originally form the thyroid placode in anterior endoderm. As lobes develop the thyroid primordial tissue branches several generations. Sox9 and Fgfr2b...

... transcription factor SOX9 locus. SOX9 is an HMG-box transcription factor that plays an essential role in chondrocyte development by directing the expression of chondrocyte-specific genes. Two of these lncRNAs are upregulated during chondrogenic differentiation of mesenchymal stem cells (MSCs). Depletion of one...

... region) identities that produce the acini and higher order ducts, respectively. Little is known about the factors that regulate progenitor expansion and specialisation in the different parts of the gland. Here, we show that Sox9 is involved in establishing the identity of the distal compartment before...

... in either chondrogenesis or in subsequent bone formation. To determine the onset of the Prmt5 cKO phenotype, we analyzed digit formation at E11.5 by in situ hybridization for Sox9 , the earliest known marker of chondrocytes ( Akiyama et al., 2005 ; Bi et al., 1999 ; Lefebvre and Smits, 2005 ; Wright...

...Xinjun He; Shinsuke Ohba; Hironori Hojo; Andrew P. McMahon An analysis of Sox9 binding profiles in developing chondrocytes identified marked enrichment of an AP-1-like motif. Here, we have explored the functional interplay between Sox9 and AP-1 in mammalian chondrocyte development. Among AP-1...

... the cardiac cushions. The transcription factor SOX9 is highly expressed in the cardiac cushion mesenchyme, and is essential for heart valve development. Loss of Sox9 in mouse cardiac cushion mesenchyme alters cell proliferation, embryonic survival, and valve formation. Despite this important role, little...

... embedded within the quadriceps tendon. At the cellular level, we show that, similar to bone eminences, the patella is formed secondarily by a distinct pool of Sox9 - and Scx -positive progenitor cells. Finally, we show that TGFβ signaling is necessary for the specification of patella progenitors, whereas...

... differentiation and male secondary sex development. XX transgenic fetal gonads showed typical testicular size and vasculature. Key ovarian markers, including Wnt4 and Foxl2 , were repressed. Sertoli cells expressing the hallmark testis-determining gene Sox9 were formed, although they did not assemble into normal...

... capacity for limb chondrogenesis in cell culture . Dev. Biol.   60 , 69 - 82 . 10.1016/0012-1606(77)90110-5 Akiyama , H. , Chaboissier , M.-C. , Martin , J. F. , Schedl , A. and De Crombrugghe , B. ( 2002 ). The transcription factor Sox9 has essential roles in successive...

... in endochondral ossification. As a negative regulator, Sox9 is essentially downregulated in terminally differentiated hypertrophic chondrocytes. However, the underlying mechanism of Sox9 silencing is undefined. Here we show that the zinc finger protein Zbtb20 regulates the terminal differentiation of hypertrophic...