...Catarina Sequeira; Lou Martha Wackerbarth; Andreia Pena; Mafalda Sá-Pereira; Cláudio A. Franco; Edgar R. Gomes ABSTRACT Skeletal muscle development is a complex process involving myoblast fusion to generate multinucleated fibers. Myonuclei first align in the center of the myotubes before migrating...

...Olga G. Jaime; Jessica Arias; Shreya Pavani; April D. Pyle; Michael R. Hicks ABSTRACT The earliest skeletal muscle progenitor cells (SMPCs) derived from human pluripotent stem cells (hPSCs) are often identified by factors expressed by a diverse number of progenitors. An early transcriptional...

...Yudai Ono; Saundra Schlesinger; Kanako Fukunaga; Shinsei Yambe; Tempei Sato; Takako Sasaki; Chisa Shukunami; Hiroshi Asahara; Masafumi Inui ABSTRACT Movement of the vertebrate body is supported by the connection of muscle, tendon and bone. Each skeletal muscle in the vertebrate body has a unique...

... and fibrillarin . Biochem. Biophys. Res. Commun. 228 , 94 - 99 . 10.1006/bbrc.1996.1621 Gokhin , D. S. and Fowler , V. M. ( 2013 ). A two-segment model for thin filament architecture in skeletal muscle . Nat. Rev. Mol. Cell Biol. 14 , 113 - 119 . 10.1038/nrm3510 Goldspink...

.... ( 2011 ). Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells . Nat. Commun. 2 , 499 . 10.1038/ncomms1508 Doyle , M. J. , Zhou , S. , Tanaka , K. K. , Pisconti , A. , Farina , N. H. , Sorrentino , B. P...

... myofibers and PAX7 + cells can also be produced in vitro from PSC ( Chal et al., 2015 ; Hicks et al., 2018 ). Such cultures offer an excellent proxy to study the development of the human myogenic lineage. Here, we report a detailed characterization of the development of human skeletal muscle in vitro...

...Megha Agarwal; Akashi Sharma; Pankaj Kumar; Amit Kumar; Anushree Bharadwaj; Masum Saini; Gabrielle Kardon; Sam J. Mathew ABSTRACT Myosin heavy chain-embryonic (MyHC-emb) is a skeletal muscle-specific contractile protein expressed during muscle development. Mutations in MYH3 , the gene encoding MyHC...

...Allison P. Kann; Robert S. Krauss ABSTRACT Skeletal muscle myofibers are large syncytial cells comprising hundreds of myonuclei, and in situ hybridization experiments have reported a range of transcript localization patterns within them. Although some transcripts are uniformly distributed...

... to migration and organized during migration to generate four hypaxial muscles. C-met Skeletal muscle Sternohyoideus Lateral line Limb Posterior hypaxial muscle Zebrafish Six1 Six4 Long-range cell migrations are vital to producing the vertebrate body plan. Migratory muscle precursors (MMPs...

... Skeletal muscle Transcription factors Epigenetics Cell signaling Differentiation National Institutes of Health 10.13039/100000002 1R01EY026202 Australian Research Council 10.13039/501100000923 FT110100573 Flinders Foundation Skeletal muscle owes its...

...-licence-1-1/ Summary: Analyses of conditional and genetic null mice reveal that HeyL and Hey1 function redundantly in MuSCs, and that HeyL requires Hes1 for effective DNA binding and biological activity. Notch Muscle stem cells Skeletal muscle Mouse Japan Society...

... skeletal muscle development, where it is a master regulator of the transition from embryonic to foetal myogenesis. Recently, Nfix was shown to be involved in adult muscle regeneration and in muscular dystrophies. Here, we have investigated the signalling that regulates Nfix expression, and show that JunB...

... Body skeletal muscles derive from the paraxial mesoderm, which forms in the posterior region of the embryo. Using microarrays, we characterize novel mouse presomitic mesoderm (PSM) markers and show that, unlike the abrupt transcriptome reorganization of the PSM, neural tube differentiation...

...Amir Mizbani; Edlira Luca; Elisabeth J. Rushing; Jan Krützfeldt MicroRNAs (miRNAs) are important regulators of skeletal muscle regeneration, but the underlying mechanisms are still incompletely understood. Here, comparative miRNA sequencing analysis of myogenic progenitor cells (MPs) and non...

... expression of JMJD3c followed by the forced expression of lineage-defining transcription factors enabled the hPSCs to activate tissue-specific genes directly. We have also shown that the introduction of JMJD3c facilitates the differentiation of hPSCs into functional hepatic cells and skeletal muscle cells...

... is implicated in myotube formation and, together with YAP/Taz/Tead1, is associated with reduced muscle fiber diameter of a subset of fast fibers. Axin2 Yap1 Wwtr1 Tead1 Skeletal muscle Muscle fibers Satellite cells The Wnt gene family encodes 19 secreted glycoproteins, which bind...

...: Satellite cell depletion prevents fiber hypertrophy in the plantaris and extensor digitorum longus muscles in mice, suggesting that satellite cells are obligatory for hypertrophic growth. Hypertrophy Plasticity Satellite cells Skeletal muscle Stem cells Mouse Norges Forskningsråd...

...Nicolas A. Dumont; Yu Xin Wang; Michael A. Rudnicki Muscle stem cells, termed satellite cells, are crucial for skeletal muscle growth and regeneration. In healthy adult muscle, satellite cells are quiescent but poised for activation. During muscle regeneration, activated satellite cells transiently...

... development Satellite cell differentiation Satellite cell quiescence Skeletal muscle Mouse Human Stem cells reside in specialized environments that regulate their behavior, called stem cell niches. Myogenic stem cells called satellite cells (mSCs) are involved in muscle growth and in regeneration...

... in vivo tagging of the nuclear envelope with biotin and the subsequent affinity purification of the labeled nuclei. In this study we successfully purified nuclei of cardiac and skeletal muscle from Xenopus using this strategy. We went on to demonstrate the utility of this approach by coupling the INTACT...