... checkpoint that defines myogenic commitment could improve hPSC differentiation to skeletal muscle. Analysis of several myogenic factors in human embryos and early hPSC differentiations found SIX1 + PAX3 + co-expression was most indictive of myogenesis. Using dCas9-KRAB hPSCs, we demonstrate that early...

... embryonic development. We showed recently that the SIX1 homeoprotein, an essential regulator of organogenesis, is also a repressor of adult cellular senescence. Alterations in the SIX/EYA pathway are linked to the human branchio-oto-renal (BOR) syndrome, a rare congenital disorder associated with defects...

... oculis-related homeobox Six1 - Six6 genes in vertebrates. Six1 , Six2 , Six4 and Six5 are expressed in the muscle lineage. Here, we tested the hypothesis that Six1 and Six4 could participate in the genesis of myogenic stem cells. We show that fewer PAX7+ cells occupy a satellite cell position between...

... taxa. Here, we demonstrate novel PSEDN roles in vivo in Drosophila hematopoiesis and in human erythropoiesis in vitro . Using Drosophila genetics, we show that PSEDN members function with GATA to block lamellocyte differentiation and maintain the prohemocyte pool. Overexpression of human SIX1...

... to migration and organized during migration to generate four hypaxial muscles. C-met Skeletal muscle Sternohyoideus Lateral line Limb Posterior hypaxial muscle Zebrafish Six1 Six4 Long-range cell migrations are vital to producing the vertebrate body plan. Migratory muscle precursors (MMPs...

... that EYA1 and SIX1 are crucial otic neuronal determination factors upstream of NEUROG1 and NEUROD1. Overexpression of both Eya1 and Six1 is sufficient to convert non-neuronal epithelial cells within the otocyst and cochlea as well as the 3T3 fibroblast cells into neurons. Strikingly, all the ectopic neurons...

...Xuguang Nie; Jianbo Sun; Ronald E. Gordon; Chen-Leng Cai; Pin-Xian Xu Dysfunction of the ureter often leads to urine flow impairment from the kidney to the bladder, causing dilation of the ureter and/or renal pelvis. Six1 is a crucial regulator of renal development: mutations in human SIX1 cause...

...Dan Zou; Derek Silvius; Bernd Fritzsch; Pin-Xian Xu Eya1 encodes a transcriptional co-activator and is expressed in cranial sensory placodes. It interacts with and functions upstream of the homeobox gene Six1 during otic placodal development. Here, we have examined their role in cranial sensory...

...Hidenori Ozaki; Kazuaki Nakamura; Jun-ichi Funahashi; Keiko Ikeda; Gen Yamada; Hisashi Tokano; Hiro-oki Okamura; Ken Kitamura; Shigeaki Muto; Hayato Kotaki; Katsuko Sudo; Reiko Horai; Yoichiro Iwakura; Kiyoshi Kawakami Six1 is a member of the Six family homeobox genes, which function as components...

... report that Six1 is required for mouse auditory system development. During inner ear development, Six1 expression was first detected in the ventral region of the otic pit and later is restricted to the middle and ventral otic vesicle within which, respectively, the vestibular and auditory epithelia form...

...Pin-Xian Xu; Weiming Zheng; Li Huang; Pascal Maire; Christine Laclef; Derek Silvius The murine Six gene family, homologous to Drosophila sine oculis ( so ) which encodes a homeodomain transcription factor, is composed of six members ( Six1-6 ). Among the six members, only the Six2 gene has been...

... hypoplasia, with severe reduction in the number of parafollicular cells and the size of the thyroid lobes and lack of fusion between the ultimobranchial bodies and the thyroid lobe. These data indicate that Eya1 also regulates mature thyroid gland formation. Furthermore, we show that Six1 expression...