... have informed our understanding of typical spermatogenesis, the molecular and cellular mechanisms underlying the maintenance and decline of spermatogenesis are not yet understood. We used single-cell RNA sequencing to generate a developmental atlas of the aging zebrafish testis. All testes contained...

... for studying this phenomenon because of the stark differences between their bilateral larval and pentaradial adult body plans. Here, we use single-cell RNA sequencing to analyze the development of Heliocidaris erythrogramma ( He ), a sea urchin species with an accelerated, non-feeding mode of larval...

..., the implementation of single-cell RNA sequencing facilitated the discovery and characterization of a molecular mechanism responsible for the segregation of dorsal neural fates during development. * Author for correspondence ( [email protected] ) Handling Editor: Debra Silver Competing interests...

... labeling of cells, isolation via fluorescence-activated cell sorting, and analysis using high-throughput techniques. We calibrated the BirthSeq method for developmental organs across three vertebrate species (mouse, chick and gecko), and utilized it for single-cell RNA sequencing and novel spatially...

...Maria Yampolskaya; Michael J. Herriges; Laertis Ikonomou; Darrell N. Kotton; Pankaj Mehta ABSTRACT Advances in single-cell RNA sequencing provide an unprecedented window into cellular identity. The abundance of data requires new theoretical and computational frameworks to analyze the dynamics...

... fluorescence-activated cell sorting (FACS) of endothelial cells (ECs) from lungs of Cxcl12 DsRed reporter mice ( Ding and Morrison, 2013 ), we performed single-cell RNA sequencing on 26,652 cells across development. Surprisingly, we found Cxcl12 expression at low levels in the subsets of previously...

... is important for activating genes during development and for histone H3 lysine 14 acetylation (H3K14ac). Loss of HBO1 and H3K14ac impaired developmental sprouting angiogenesis and reduced pathological EC overgrowth in the retinal endothelium. Single-cell RNA sequencing of retinal ECs revealed an increased...

... bioinformatic strategy integrating TF expression levels and regulon activities from single cell RNA-sequencing data, we find evidence that TCF4 interacts with non-bHLH TFs and modulates their transcriptional activity in Satb2 + intercortical projection neurons. Notably, this network comprises regulators linked...

... http://www.biologists.com/user-licence-1-1/ Summary: Single-cell RNA-sequencing identifies a repertoire of genetically defined developmental states conserved by embryonic and postembryonic retinal neurogenesis. Developmental state Single-cell RNA sequencing Retinal progenitor cells...

...Kun Tan; Hye-Won Song; Miles F. Wilkinson ABSTRACT Pro-spermatogonia (SG) serve as the gateway to spermatogenesis. Using single-cell RNA sequencing (RNAseq), we studied the development of ProSG, their SG descendants and testicular somatic cells during the perinatal period in mice. We identified...