...Mingyuan Zhu; Isaiah W. Taylor; Philip N. Benfey ABSTRACT Understanding the development of tissues, organs and entire organisms through the lens of single-cell genomics has revolutionized developmental biology. Although single-cell transcriptomics has been pioneered in animal systems, from...

...; Nikolay Ninov ABSTRACT Regeneration-competent species possess the ability to reverse the progression of severe diseases by restoring the function of the damaged tissue. However, the cellular dynamics underlying this capability remain unexplored. Here, we have used single-cell transcriptomics to map de...

.... Approximately 2500 sorted cells were directly loaded in sheath fluid onto one lane of a Chromium 10X chip (10X Genomics) and libraries prepared using the normal workflow for Single Cell 3´ v3 libraries (10X Genomics). Libraries were sequenced on the NextSeq 500 platform (Illumina), and the raw format base call...

... to organoids, single-cell technology and clinical applications. * Authors for correspondence ( [email protected] ; [email protected] ) Competing interests The authors declare no competing or financial interests. © 2021. Published by The Company of Biologists Ltd 2021...

... the transcription factors TCF21 and WT1. Here, we show that this cell heterogeneity is conserved in human epicardium, regulated by BNC1 and associated with cell fate and function. Single cell RNA sequencing of epicardium derived from human pluripotent stem cells (hPSC-epi) revealed that distinct epicardial...

... populations per experiment. A new generation of tools that use molecular recording methods integrated with single cell profiling technologies may provide a solution. Here, we summarize recent breakthroughs in these technologies, outline experimental and computational challenges, and discuss biological...

...Samantha A. Morris; Allon Klein; Barbara Treutlein ABSTRACT Fueled by recent advances in single cell biology, we are moving away from qualitative and undersampled assessments of cell identity, toward building quantitative, high-resolution cell atlases. However, it remains challenging to precisely...

...Urs Mayr; Denise Serra; Prisca Liberali; Allon Klein; Barbara Treutlein ABSTRACT Complex 3D tissues arise during development following tightly organized events in space and time. In particular, gene regulatory networks and local interactions between single cells lead to emergent properties...

... in individual cells during this transition is unclear. Using a FRET-based biosensor, we monitored ERK signalling dynamics of single mouse ESCs during differentiation. ERK activity was highly heterogeneous, with considerable variability in ERK signalling between single cells within ESC colonies. Different...

... of spermatogenesis. However, a systematic understanding of this process is still limited. To resolve cellular and molecular heterogeneity in this process, we profiled single cell transcriptomes of undifferentiated germ cells from neonatal mouse testes and employed unbiased clustering and pseudotime ordering analysis...

... single-cell transcriptomics to characterize gene expression in different cell populations, and to study individual cell dynamics and lineage trajectories during development. Single-cell transcriptome analyses of 6414 cells from five individual specimens identified 11 initial clusters of specific renal...

...Giuliano G. Stirparo; Thorsten Boroviak; Ge Guo; Jennifer Nichols; Austin Smith; Paul Bertone ABSTRACT Single-cell profiling techniques create opportunities to delineate cell fate progression in mammalian development. Recent studies have provided transcriptome data from human pre-implantation...

...Mike Adam; Andrew S. Potter; S. Steven Potter Single-cell RNA-seq is a powerful technique. Nevertheless, there are important limitations, including the technical challenges of breaking down an organ or tissue into a single-cell suspension. Invariably, this has required enzymatic incubation at 37°C...

... the tantalizing prospect of cataloging cell type diversity and developmental dynamics in a quantitative and comprehensive manner. Here, we review how single-cell RNA sequencing has provided key insights into mammalian developmental and stem cell biology, emphasizing the analytical approaches that are specific...

... and adult stem cell biology were discussed, including their respective niches in ageing, disease and regeneration. Many presentations focused on emerging technologies, including single-cell analysis, in vitro organogenesis and direct reprogramming. Here, we summarize the data presented at this exciting...

... modeling predicted that a small fraction of lead cells that detect directional information is optimal for successful stream migration. Single-cell profiling then revealed a unique molecular signature that is consistent and stable over time in a subset of lead cells within the most advanced portion...

... financial interests. 25 9 2012 © 2013. Published by The Company of Biologists Ltd RNA visualization Cell state Differentiation Fluorescent in situ hybridization Single cell Single molecule The primary unit of biological control is the cell. Measurement...