... interests statement The authors declare no competing financial interests. 1 2 2010 © 2010. 2010 Left-right asymmetry Midline Conjoined twins Heterotaxia Organizer Siamois Xenopus During development, embryos undergo an exquisitely complex biophysical program to acquire...

...@hgu.mrc.ac.uk ) 9 12 2008 © 2009. 2009 Cancer DNA methylation Kaiso Siamois TCF3 Chromatin The BTB/POZ transcriptional factor xKaiso is a bimodal DNA-binding protein that is reported to specifically bind methyl-CpGs, or a CTGCNA consensus DNA sequence ( Ruzov et al., 2004 ; Park...

... abnormalities associated with xKaiso-depleted Xenopus laevis embryos are independent of the putative CTGCNA-dependent DNA-binding function of xKaiso. Our analysis suggests that xKaiso does not play a role in the regulation of either xWnt11 or Siamois, key signalling molecules in the Wnt pathway during X. laevis...

... as a transcriptional activator. Mix . 1 also has a role in endoderm formation. It cooperates with the dorsal vegetal homeobox gene Siamois to activate the endodermal markers edd, Xlhbox8 and cerberus in animal caps. Conversely, vegetal overexpression of enRMix . 1 , an antimorphic Mix . 1 mutant, leads to a loss...

... siamois ( Xsia ) suggesting that Xtwn and Xsia comprise a new subclass of homeobox genes important in dorsal axis specification. We find that Xtwn is able to activate the Spemann organizerspecific gene goosecoid ( gsc ) via direct binding to a region of the gsc promoter previously shown to mediate Wnt...

... region of the fertilised egg during cortical rotation. While the molecular identity of the determinants is unknown, there are dorsal factors in the vegetal cortical cytoplasm (VCC). Here, we show that the VCC factors, when injected into animal cells activate the zygotic genes Siamois and Xnr3...

...., Noro, C. Y. and Wylie, C. (1994) Cell 79, 791-803). (2) A transcription factor with potent dorsalizing activity, Siamois, is expressed within the Nieuwkoop Center ( Lemaire, P., Garrett, N. and Gurdon, J. B. (1995) Cell 81, 85-94). We have used these two properties of the Nieuwkoop Center to evaluate...

...Gilles Carnac; Laurent Kodjabachian; J. B. Gurdon; Patrick Lemaire ABSTRACT Siamois , a Xenopus zygotic homeobox gene with strong dor-salising activity, is expressed in the dorsal-vegetal organiser known as the Nieuwkoop centre. We show that, in contrast to Spemann organiser genes such as goosecoid...