... that there is a small contribution of SHH -expressing cells to the posterior ulna, posterior carpals and digit 3. We establish that although the majority of the ulna develops in response to paracrine SHH signalling in both the chicken and mouse, there are differences in the contribution of SHH -expressing cells between...

... in ureter specification by generating a mesenchymal domain around the distal ureteric bud where SHH and BMP4 signaling can occur. We performed whole-mount RNA in situ hybridizations following a standard procedure with digoxigenin-labeled antisense riboprobes ( Wilkinson and Nieto, 1993 ). Stained...

... cell proliferation and suppressing nucleus pulposus cell death through augmenting FGFR1-SHH signaling. These findings reveal that Ccn2a plays a central role in IVD homeostasis and regeneration, which could be exploited for therapeutic intervention in degenerated human discs. * Author...

...Jingyue Xu; Han Liu; Yu Lan; Rulang Jiang ABSTRACT The tongue is a highly specialized muscular organ with diverse cellular origins, which provides an excellent model for understanding mechanisms controlling tissue-tissue interactions during organogenesis. Previous studies showed that SHH signaling...

.... We have recently shown that epithelial FGFR2 signaling regulates stratification and differentiation of the epithelial compartment by enhancing epithelial Shh expression, and mesenchymal SHH and BMP4 activity. Here, we show that FGFR1 and FGFR2 expression in the mesenchymal primordium impinges...

... negatively on epithelial expression of Shh and its mesenchymal effector gene Bmp4 . Activation of SHH or BMP4 signaling largely rescued the cellular defects of mutant ureters in explant cultures. Conversely, inhibition of SHH or BMP signaling in wild-type ureters recapitulated the mutant phenotype in a dose...

... target genes in the anterior limb bud mesenchyme. Analysis of differentially expressed genes shared between Smad4 - and Shh -deficient limb buds corroborates this function of SMAD4 and also reveals the repressive effect of SMAD4 on posterior genes that are upregulated in response to SHH signaling...

...Nora Mecklenburg; Izabela Kowalczyk; Franziska Witte; Jessica Görne; Alena Laier; Tamrat M. Mamo; Hannes Gonschior; Martin Lehmann; Matthias Richter; Anje Sporbert; Bettina Purfürst; Norbert Hübner; Annette Hammes ABSTRACT Pathogenic gene variants in humans that affect the sonic hedgehog (SHH...

...), that is required for the progression of tooth development. IK cell dynamics demonstrate the hallmarks of a signaling center: cell cycle exit, condensation and eventual silencing through apoptosis. IK initiation and maturation are defined by the juxtaposition of cells with high Wnt activity to Shh -expressing non...

... proliferation and patterning is identified involving protein-protein interactions. Dental epithelial stem cells Pitx2 Shh Signaling centers Sox2 Transcriptional regulation Stem cell niche Craniofacial/tooth development Irx1 Enamel knot University of Iowa 10.13039/100008893 EB025873...

..., we show that Sonic hedgehog (Shh) signalling in the embryonic chick wing bud specifies positional information required for the formation of adult flight feathers in a defined spatial and temporal sequence that reflects their different identities. We also reveal that Shh signalling is interpreted...

...Iain Williamson; Lauren Kane; Paul S. Devenney; Ilya M. Flyamer; Eve Anderson; Fiona Kilanowski; Robert E. Hill; Wendy A. Bickmore; Laura A. Lettice ABSTRACT Topologically associating domains (TADs) have been proposed to both guide and constrain enhancer activity. Shh is located within a TAD known...

...Tanya S. Corman; Solsire E. Bergendahl; Douglas J. Epstein ABSTRACT Sonic hedgehog (Shh) plays well characterized roles in brain and spinal cord development, but its functions in the hypothalamus have been more difficult to elucidate owing to the complex neuroanatomy of this brain area. Here, we...

... thymic epithelial cells (TECs) promotes positive selection and differentiation from CD4 + CD8 + to CD4 + CD8 − single-positive (SP4) cells in the fetal thymus and that Gli3 represses Shh . Constitutive deletion of Gli3 , and conditional deletion of Gli3 from TECs, reduced differentiation to SP4, whereas...

... differentiate into mammillary cells. A stable population of Fgf10 + progenitors is retained within the tuberal domain; a subset of these gives rise to the tuberal infundibulum – the precursor of the posterior pituitary. Pharmacological approaches reveal that Shh signalling promotes the growth...

... taste bud cells, but not progenitor proliferation, is interrupted in mice treated with a hedgehog (Hh) pathway inhibitor (HPI), and that gustatory nerves are a source of sonic hedgehog (Shh) for taste bud renewal. Additionally, epithelial taste precursor cells express Shh transiently, and provide...

... + progenitor population relies on the activity of the Shh, Wnt and Fgf signaling pathways. Wnt and Fgf function additively to generate dHb progenitors. Surprisingly, Wnt signaling also negatively regulates fgf8a , confining expression to a discrete dorsal diencephalic domain. Moreover, the Wnt and Fgf pathways...

.../ Summary: Sox17 haploinsufficiency in the gallbladder induces ectopic cystic duct-like epithelia and defective smooth muscle formation by reduced Shh signals. Gallbladder Biliary atresia Sox17 Shh Ministry of Education, Culture, Sports, Science, and Technology 10.13039/501100001700...

... and adult urothelial progenitors. Without Eed , the obligatory subunit of PRC2, embryonic urothelial progenitors demonstrate reduced proliferation with concomitant dysregulation of genes including Cdkn2a ( p16 ), Cdkn2b ( p15 ) and Shh. These mutants display premature differentiation of keratin 5-positive...

...Joseph Pickering; Matthew Towers In an influential model of pattern formation, a gradient of Sonic hedgehog (Shh) signalling in the chick wing bud specifies cells with three antero-posterior positional values, which give rise to three morphologically different digits by a self-organizing mechanism...