... chordates, genetic studies of Crabp function have revealed few essential functions. We have generated loss-of-function mutations in all four zebrafish Crabps and find essential roles for Crabp2 proteins in gonad development and sex determination. Transgenic RA reporters show strong RA responses in germ...

... variable gonadal sex reversal phenotype in the fetal period, characterised by appearance of ovarian tissue. To investigate this sex reversal further, we used single-cell RNA-seq to examine the transcriptomes of XY Znrf3 -deficient gonads during the mouse sex-determining period. Analyses of cell...

...Corey Bunce; Lindsey Barske; Gloria Zhang; Blanche Capel ABSTRACT During mammalian development, gonadal sex determination results from the commitment of bipotential supporting cells to Sertoli or granulosa cell fates. Typically, this decision is coordinated across the gonad to ensure commitment...

... of the supporting cell sex lineage. TGIF1 Ovarian cortex Juxtacortical medulla Gonadal development Sex determination Chicken embryo References Andrews , J. E. , Smith , C. A. and Sinclair , A. H. ( 1997 ). Sites of estrogen receptor and aromatase expression in the chicken embryo...

... maintains female identity in Drosophila germ cells . Nat. Commun.   9 , 4155 . 10.1038/s41467-018-06697-x Staab , S. , Heller , A. and Steinmann-Zwicky , M. ( 1996 ). Somatic sex-determining signals act on XX germ cells in Drosophila embryos . Development   122 , 4065 - 4071...

... was examined. These results show that the early differentiation of the cortex can be independent of the sex of the medulla and points to oestrogen as the key inducer of this process. To upregulate oestrogen in ZZ embryos after sex determination, we injected β-oestradiol in ovo at D7-7.5 (HH31...

... to the recruitment of the dosage compensation complex to the X chromosome in Drosophila . MSL CLAMP Sex determination Transcription factor C2H2 zinc finger CXC Russian Science Foundation http://dx.doi.org/10.13039/501100006769 17-74-20155 In Drosophila males, a multisubunit dosage...

... that changes unrelated to Mid underlie the evolution of gamete dimorphism. Chlamydomonas Gonium Pleodorina RWP-RK Volvox Sex determination National Institutes of Health 10.13039/100000002 GM 078376 Gamete size dimorphism (anisogamy or oogamy) is a nearly ubiquitous...

... underlying their female-specific generation. We demonstrate that programmed cell death (PCD) eliminates FS-Ilp7 motoneurons in males, and that this requires male-specific splicing of the sex-determination gene fruitless ( fru ) into the Fru MC isoform. However, in females, fru alleles that only generate Fru...

... challenging in the context of developing tissues where material is limited and cell types are mixed. To identify regulatory sites during sex determination, we subjected Sertoli cells from mouse fetal testes to DNaseI-seq and ChIP-seq for H3K27ac. DNaseI-seq identified putative regulatory sites around genes...

... was this mouse so special? The mouse in question was a chromosomal female (XX) made male by the presence of a transgene containing the Y chromosome gene Sry . This sex-reversal provided clear experimental proof that Sry was the elusive mammalian sex-determining gene. Twenty-five years on, we reflect on what...

...Liang Zhao; Terje Svingen; Ee Ting Ng; Peter Koopman Genes related to Dmrt1 , which encodes a DNA-binding DM domain transcription factor, act as triggers for primary sex determination in a broad range of metazoan species. However, this role is fulfilled in mammals by Sry , a newly evolved gene...

...Quan Wu; Kurumi Fukuda; Michael Weinstein; Jonathan M. Graff; Yumiko Saga The sex of primordial germ cells (PGCs) is determined in developing gonads on the basis of cues from somatic cells. In XY gonads, sex-determining region Y (SRY) triggers fibroblast growth factor 9 (FGF9) expression in somatic...

...Christian Larney; Timothy L. Bailey; Peter Koopman Mammalian sex determination hinges on the development of ovaries or testes, with testis fate being triggered by the expression of the transcription factor sex-determining region Y ( Sry ). Reduced or delayed Sry expression impairs testis...

..., 19700 Helix Drive, Ashburn, VA 20147, USA † Author for correspondence ( [email protected] ) Competing interests statement The authors declare no competing financial interests. 22 4 2011 © 2011. 2011 DamID Doublesex Evolution Sex determination Transcription...

... at times when HES family members are active and limits Hey and HES family activity during sex determination, segmentation and neurogenesis. We show that Dgrn interacts with the Notch signaling pathway by it antagonizing the activity of E(spl)-C proteins. dgrn null mutants are female sterile, producing...

...Kenichi Kashimada; Peter Koopman SRY, the mammalian Y-chromosomal testis-determining gene, induces male sex determination. Recent studies in mice reveal that the major role of SRY is to achieve sufficient expression of the related gene Sox9, in order to induce Sertoli cell differentiation, which...

...Shin Yoshimoto; Nozomi Ikeda; Yumi Izutsu; Tadayoshi Shiba; Nobuhiko Takamatsu; Michihiko Ito A Y-linked gene, DMY/dmrt1bY , in teleost fish medka and a Z-linked gene, DMRT1 , in chicken are both required for male sex determination. We recently isolated a W-linked gene, DM-W , as a paralogue...

... the tools for dissecting how neurogenesis and neuronal differentiation are modulated by the Drosophila sex-determination regulatory genes to produce nervous system sexual dimorphisms. Here we report studies aimed at illuminating the basis of the sexual dimorphic axonal projection patterns of foreleg...

... animals (1X:2A=0.5) develop as males. TRA-1, the worm ortholog of Drosophila Cubitus interruptus and mammalian Gli (Glioma-associated homolog) proteins, is the terminal transcription factor of the C. elegans sex-determination pathway, which specifies hermaphrodite fate by repressing male-specific genes...