...Anish Dattani; Tao Huang; Corin Liddle; Austin Smith; Ge Guo ABSTRACT Propagation of human naïve pluripotent stem cells (nPSCs) relies on the inhibition of MEK/ERK signalling. However, MEK/ERK inhibition also promotes differentiation into trophectoderm (TE). Therefore, robust self-renewal requires...

... of nephron progenitors during renal development. Organogenesis Tissue-specific progenitors Pycr1/Pycr2 Self-renewal Differentiation Development Metabolism Receptor tyrosine kinase signaling Intracellular signaling cascades Academy of Finland http://dx.doi.org/10.13039/501100002341...

... to study lineage specification of placental tissues; however, the regulatory network controlling self-renewal remains elusive. Here, we present a single-cell atlas of human trophoblast development from zygote to mid-gestation together with single-cell profiling of hTSCs. We determine the transcriptional...

... ABSTRACT Despite four decades of effort, robust propagation of pluripotent stem cells from livestock animals remains challenging. The requirements for self-renewal are unclear and the relationship of cultured stem cells to pluripotent cells resident in the embryo uncertain. Here, we avoided using feeder...

... show that a self-renewing cell type – if defined as suggested by this framework – must reside at the top of any homeostatic renewing lineage hierarchy, and only there. This architecture arises as a natural consequence of homeostasis, and indeed is the only possible way that lineage architectures can...

... variability and improving the efficiency, reproducibility and reliability of embryonic stem cell culture. Differentiation Embryonic stem cells Pluripotency Self-renewal Mouse embryonic stem (ES) cells are cell lines derived from the pre-implantation epiblast of mouse embryos ( Boroviak et al...

..., are generated. Recent studies have significantly modified earlier views on the mechanisms of NSC self-renewal and neurogenesis in the adult brain. Here, we discuss the molecular control, heterogeneity, regional specification and cell division modes of V-SVZ NSCs, and draw comparisons with NSCs in the SGZ. We...

...Caroline Dillard; Karine Narbonne-Reveau; Sophie Foppolo; Elodie Lanet; Cédric Maurange ABSTRACT Whether common principles regulate the self-renewing potential of neural stem cells (NSCs) throughout the developing central nervous system is still unclear. In the Drosophila ventral nerve cord...

... for myogenic commitment and differentiation, timely repression of their activity is necessary for the self-renewal and maintenance of muscle stem cells (satellite cells). Here, we define Ascl2 as a novel inhibitor of MRFs. During mouse development, Ascl2 is transiently detected in a subpopulation of Pax7...

...Xing Ma; Yingying Han; Xiaoqing Song; Trieu Do; Zhihao Yang; Jianquan Ni; Ting Xie Stem cells in adult tissues are constantly exposed to genotoxic stress and also accumulate DNA damage with age. However, it remains largely unknown how DNA damage affects both stem cell self-renewal...

... that facilitates the membrane localization of GLP-1 /Notch. Self-renewal Differentiation STRIPAK Far complex Germ cells Endoplasmic reticulum (ER) is the site of synthesis and folding of all secreted and transmembrane proteins, including the ligands and receptors of some key signaling pathways...

...Jun Yu; Xiang Lan; Xia Chen; Chao Yu; Yiwen Xu; Yujuan Liu; Lingna Xu; Heng-Yu Fan; Chao Tong The homeostasis of self-renewal and differentiation in stem cells is controlled by intrinsic signals and their niche. We conducted a large-scale RNA interference (RNAi) screen in Drosophila testes...

... fate decision Stem cell Self-renewal Differentiation Whereas differential exposure to environmental cues can instruct asymmetric fates, varying mitotic spindle orientation can produce intrinsically asymmetric cell divisions by segregating fate determinants unequally in the daughter cells...

... re-enter the cell cycle to proliferate and subsequently exit the cell cycle to differentiate or self-renew. Recent studies have demonstrated that satellite cells are heterogeneous and that subpopulations of satellite stem cells are able to perform asymmetric divisions to generate myogenic progenitors...

...Yuwen Li; Jiao Liu; Wencheng Li; Aaron Brown; Melody Baddoo; Marilyn Li; Thomas Carroll; Leif Oxburgh; Yumei Feng; Zubaida Saifudeen Contrary to its classic role in restraining cell proliferation, we demonstrate here a divergent function of p53 in the maintenance of self-renewal of the nephron...

... goblet cell lineage that partially mimics Notch loss-of-function defects. Finally, we provide evidence that Bmi1 contributes to ISC self-renewal. Fig. 5. The Notch inhibition phenotype mimics that of Bmi1 deficiency with respect to the self-renewal and DNA repair capacity of ISCs...

...Chen-yan Ma; Mao-jin Yao; Qi-wei Zhai; Jian-wei Jiao; Xiao-bing Yuan; Mu-ming Poo The balance between self-renewal and differentiation of adult neural stem cells (aNSCs) is essential for the maintenance of the aNSC reservoir and the continuous supply of new neurons, but how this balance is fine...

...Sowmya Parameswaran; Xiaohuan Xia; Ganapati Hegde; Iqbal Ahmad In vertebrate retina, histogenesis occurs over an extended period. To sustain the temporal generation of diverse cell types, retinal progenitor cells (RPCs) must self-renew. However, self-renewal and regulation of RPCs remain poorly...

... and differentiation of LRCs in vitro and impaired satellite cell self-renewal after muscle injury. By contrast, loss of p21 cip1 only affected nonLRCs, in which myogenic commitment was inhibited. Our results provide evidence that restriction of self-renewal potential to LRCs is established early in life...

... cell self-renewal and differentiation; they divide asymmetrically to self-renew and generate the neurons and glia of the CNS. Here we report the identification of midlife crisis ( mdlc ; CG4973 ) as a gene required for the maintenance of neuronal differentiation and for neuroblast proliferation...