..., leading to a short ventricle and outflow tract abnormalities. Heart development Second heart field Morphogenesis Congenital heart disease FGF signaling National Institutes of Health R35 HL135778 New York State Department of Health http://dx.doi.org/10.13039/100004856...

... of Mef2c -AHF genetic lineage tracing (red) in control and Tbx20 EH1Mut embryos. Sections are co-stained with TPM1 (green). Data are mean±s.e.m. ** P ≤0.01 (Welch's t -test). aSHF, anterior second heart field; AT; atria; HT, heart tube; OFT, outflow tract; pSHF, posterior second heart field; RV, right...

... simultaneously with growth of the heart. Cardiac growth during early heart morphogenesis is driven by deployment of cells from the second heart field (SHF) into both poles of the heart. Laminin is a core component of the extracellular matrix and, although mutations in laminin subunits are linked with cardiac...

..., is expressed by second heart field (SHF) progenitors at time-points that are crucial in SHF biology. Dll4-mediated Notch signaling is required for maintaining an adequate pool of SHF progenitors, such that Dll4 knockout results in a reduction in proliferation and an increase in apoptosis. A reduced SHF...

... of the later-differentiating outflow tract (OFT). We show that improper specification and proliferation of second heart field (SHF) progenitors in zebrafish lazarus ( lzr ) mutants, which lack the transcription factor Pbx4, produces enlarged hearts owing to an increase in ventricular and smooth muscle cells...

...Ding Li; Allyson Angermeier; Jianbo Wang ABSTRACT The second heart field (SHF) harbors progenitors that are important for heart formation, but little is known about its morphogenesis. We show that SHF population in the mouse splanchnic mesoderm (SpM-SHF) undergoes polarized morphogenesis...

... , C. E. and Burns , C. G. ( 2013 ). Zebrafish second heart field development relies on progenitor specification in anterior lateral plate mesoderm and nkx2.5 function . Development   140 , 1353 - 1363 . 10.1242/dev.088351 Hami , D. , Grimes , A. C. , Tsai , H.-J...

... transcription factor is a central regulator of cardiac development and is expressed in both the first and second heart fields (FHF and SHF). We have previously revealed essential functions of nkx2.5 and nkx2.7 , two Nkx2-5 homologs expressed in zebrafish cardiomyocytes, in maintaining ventricular identity...

...Noelle Paffett-Lugassy; Natasha Novikov; Spencer Jeffrey; Maryline Abrial; Burcu Guner-Ataman; Srinivasan Sakthivel; Caroline E. Burns; C. Geoffrey Burns During mammalian embryogenesis, cardiac progenitor cells constituting the second heart field (SHF) give rise to the right ventricle and primitive...

.... Initially, cardiomyocytes derived from first heart field (FHF) progenitors assemble the linear heart tube. Thereafter, second heart field (SHF) progenitors differentiate into cardiomyocytes that are accreted to the poles of the heart tube over a well-defined developmental window. Although heart tube...

... CASZ1 is expressed in murine cardiomyocytes. Genetic fate mapping with an inducible Casz1 allele demonstrates that CASZ1-expressing cells give rise to cardiomyocytes in the first and second heart fields. We show through the generation of a cardiac conditional null mutation that Casz1 is essential...

.... Published by The Company of Biologists Ltd 2015 SUMMARY: The study of zebrafish embryos deficient for Gpc4 reveals that it is essential for efficient cardiomyocyte differentiation, providing insights into how mutations in glypicans can cause congenital heart defects. Second heart field Wnt...

...Wen Luo; Xia Zhao; Hengwei Jin; Lichan Tao; Jingai Zhu; Huijuan Wang; Brian A. Hemmings; Zhongzhou Yang Second heart field (SHF) progenitors exhibit continued proliferation and delayed differentiation, which are modulated by FGF4/8/10, BMP and canonical Wnt/β-catenin signaling. PTEN-Akt signaling...

... show that Arid3b is expressed in the myocardium of the tubular heart and in second heart field progenitors. Arid3b -deficient embryos show cardiac abnormalities, including a notable shortening of the poles, absence of myocardial differentiation and altered patterning of the atrioventricular canal...

.... Proliferation assays reveal that hand2 can impact cardiomyocyte production by promoting division of late-differentiating cardiac progenitors within the second heart field. Additionally, our data suggest that hand2 can influence cardiomyocyte production by altering the patterning of the anterior lateral plate...

...Burcu Guner-Ataman; Noelle Paffett-Lugassy; Meghan S. Adams; Kathleen R. Nevis; Leila Jahangiri; Pablo Obregon; Kazu Kikuchi; Kenneth D. Poss; Caroline E. Burns; C. Geoffrey Burns Second heart field (SHF) progenitors perform essential functions during mammalian cardiogenesis. We recently identified...

... located medially relative to atrial progenitors ( Schoenebeck et al., 2007 ). The portion of the heart field that gives rise to the initial heart tube has been called the first heart field, whereas the second heart field is added later. In mouse, these two populations of cardiac progenitors...

...Fabienne Lescroart; Robert G. Kelly; Jean-François Le Garrec; Jean-François Nicolas; Sigolène M. Meilhac; Margaret Buckingham Head muscle progenitors in pharyngeal mesoderm are present in close proximity to cells of the second heart field and show overlapping patterns of gene expression. However...

... that Hedgehog (Hh) signaling marked a subset of cardiac progenitors specific to the atrial septum and the pulmonary trunk in the mouse. Using genetic inducible fate mapping with Gli1 CreERT2 , we marked Hh-receiving progenitors in anterior and posterior second heart field splanchnic mesoderm between E8 and E10...

...Tessa Peterkin; Abigail Gibson; Roger Patient Over the past few years it has become clear that over half of the mammalian heart derives from outside the heart field as originally defined. Such a second heart field, however, has not been described in zebrafish, which could explain its smaller, two...