..., we present a complete multi-sample preparation, imaging, processing and analysis workflow to determine the development of the vascular volume in zebrafish. Up to five live embryos were mounted and imaged simultaneously over several days using selective plane illumination microscopy (SPIM...

... tissue. We found that the bright-field contrast of unstained specimens in a selective plane illumination microscopy (SPIM) setup can be exploited for in vivo tomographic reconstructions of the three-dimensional anatomy of zebrafish, without causing phototoxicity. We report multimodal imaging of entire...

...Anna Kaufmann; Michaela Mickoleit; Michael Weber; Jan Huisken Light sheet microscopy techniques, such as selective plane illumination microscopy (SPIM), are ideally suited for time-lapse imaging of developmental processes lasting several hours to a few days. The success of this promising technology...

... migration rather than oriented cell division, and that ErbB2 cell-autonomously regulates this process. * Author for correspondence ( [email protected] ) Competing interests statement 20 9 2010 © 2010. Cardiac trabeculation Endocardium SPIM ErbB2 Zebrafish...

... of the valve. Zebrafish embryos were treated with 4 μM AG1478, an ErbB receptor inhibitor, or 1% DMSO from 56-72 hpf and imaged with SPIM ( A-C ) or stained with rhodamine-phalloidin and imaged with confocal microscopy( D , E ). Retrograde flow of blood cells per beat was quantified for animals treated with 1...