..., validating the presented method and affirming its power in early implantation site studies as our SHG-derived data (decreased decidual nest volume) and THG-derived data (smaller conceptuses) of Smad cKO implantation sites suggest aberrant embryo development consistent with the reported pregnancy failures...

... segment of the nephron. Bmp7 appears to act through Smad 1,5,9(8), p38 and JNK MAP kinase. In the absence of Bmp7, nephrons undergo a hypertrophic process that involves p38. Following a global inactivation of Bmp7 , we also see evidence for Bmp7-driven growth of the nephron postnatally. Thus, we identify...

... interpret gradients of positional signals relative to their neighbours to position the primitive streak, establishing bilateral symmetry. Morphogen Chick embryo Polarity Primitive streak Gastrulation Cell communication SMAD Wellcome Trust http://dx.doi.org/10.13039/100010269 107055...

... is that the neonatal kidney has the capacity to control interstitial cell proliferation when the target number has been reached. In this study, we define the consequences of inactivating the TGFβ/Smad response in the mouse interstitial cell lineage. We find that pathway inactivation through loss of Smad4 leads...

... Seuntjens ABSTRACT The transcription factor Zeb2 controls fate specification and subsequent differentiation and maturation of multiple cell types in various embryonic tissues. It binds many protein partners, including activated Smad proteins and the NuRD co-repressor complex. How Zeb2 subdomains support...

... is proposed to be crucial for nuclear movement, is regulated by Nodal signaling and nuclear envelope proteins. The non-Smad JNK signaling pathway, which is downstream of Nodal signaling, regulates nuclear movement independently of the Smad pathway, and this nuclear movement is associated with Smad signal...

... the environment and proliferative germ cell accumulation. Neuronal DAF-7/TGFβ causes a DAF-1/TGFβR signaling cascade in the gonadal distal tip cell (DTC), the germline stem cell niche, where it negatively regulates a DAF-3 SMAD and DAF-5 Sno-Ski. LAG-2, a founding DSL ligand family member, is produced in the DTC...

... cell type-specific targets and functions: it acts with SMAD1 to promote mesoderm specification, while in the endoderm it interacts with SMAD2/3. SMAD T-BOX Embryonic stem cells Gastrulation Gene regulatory networks Human The three primary germ layers (ectoderm, mesoderm and endoderm...

... signaling is required for proper craniofacial morphogenesis. In this study, we found that augmented BMP-Smad signaling through BMPR1A in neural crest cells leads to an increase in p53-mediated apoptosis in developing nasal cartilage. We documented that 55% of ca-Bmpr1a:P0-Cre mice experience neonatal...

.... Neurogenesis Glia Smad Id1 Mouse Several signaling pathways regulate the production and differentiation of glial cells in the central nervous system of vertebrates. For the astrocyte lineage, the best characterized of these signaling pathways is CNTF/LIF, acting through the gp130 receptor...

... ) Competing interests The authors declare no competing financial interests. 30 12 2013 16 9 2014 © 2014. Published by The Company of Biologists Ltd 2014 TGFβ Foxh1 Smad Mesendoderm Morphogenesis Cell fate Oct4 Pou5f1 Xenopus tropicalis Signaling Genomics ChIP-seq RNA...

... In the embryonic CNS, development of myelin-forming oligodendrocytes is limited by bone morphogenetic proteins, which constitute one arm of the transforming growth factor-β (Tgfβ) family and signal canonically via Smads 1/5/8. Tgfβ ligands and Activins comprise the other arm and signal via Smads 2/3...

... Competing interests The authors declare no competing financial interests. 18 4 2013 26 9 2013 © 2013. Published by The Company of Biologists Ltd 2013 Cell fate Eye Gsk3β Mitf Optic vesicle patterning Optic cup Retinal development Smad Vsx2 During...

... autoregulatory pathway through the formation of an unusual BMP4-induced Smad complex containing Smad2 and Smad5. Quantitative expression analysis supports that ectopic Nodal expression in the Smad5 mutant amnion is induced by the Nodal autoregulatory loop and a slow positive-feedback loop. The latter involves...

... prevents the ectopic expression of lateral ectoderm markers, independently of its role in neural specification. We then investigate the temporal participation of BMP/Smad signaling and suggest a later involvement in neural plate border development, likely due to widespread FGF/MAPK activity in the gastrula...

..., a mediator of Hh signalling, and activated Smads (pSmads), mediators of bone morphogenic protein (BMP) signalling, in vivo. We found a strict negative correlation between nuclear accumulation of pSmad, and eng2a expression in myotomal cells and show that abrogation of pSmad accumulation results in activation...

... conserved among tetrapods and unexpectedly directed all the Emx2 expression in forebrain: caudal forebrain primordium at E8.5, dorsal telencephalon at E9.5-E10.5 and the cortical ventricular zone after E12.5. Otx, Tcf, Smad and two unknown transcription factor binding sites were essential to all...

... understanding pathways regulating nephron progenitor renewal. Surprisingly, we have found that the NZ is essentially unresponsive to SMAD-mediated transcriptional activation( Blank et al., 2008 ). This intriguing finding prompted us to investigate whether alternate signaling pathways, activated by BMP7...

...Kelsey N. Retting; Buer Song; Byeong S. Yoon; Karen M. Lyons Bone morphogenetic protein (BMP) signaling is required for endochondral bone formation. However, whether or not the effects of BMPs are mediated via canonical Smad pathways or through noncanonical pathways is unknown. In this study we...

... of e-cadherin , thereby perturbing cell movements during convergent extension, epiboly and node formation. Thus, although the role of Smad proteins in mediating Nodal signaling is well-documented, the functional characterization of Ttrap provides insight into a novel Smad partner that plays...